HIPHOP chemogenomics database

YGL095C / VPS45

Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL095C / VPS45

Click on Significant Values for Screen Details

Top fitness defect scores for YGL095C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1826	st055655	13.70	41.6 µM	2.72E-20	ERG2	4	6.69E-43
2	SGTC_2514	salinomycin	12.50	42.7 µM	3.36E-17		3	4.66E-36
3	SGTC_264	promethazine	12.10	12.5 mM	3.51E-16	DNA intercalators	6	8.36E-34
4	SGTC_1672	st016598	11.90	6.5 µM	8.49E-16	endomembrane recycling	5	5.90E-33
5	SGTC_2242	6630449	11.60	61.3 µM	4.07E-15	Golgi	5	1.88E-31
6	SGTC_1679	4130-1276	11.20	6.7 µM	2.95E-14	Golgi	6	1.50E-29
7	SGTC_2697	mebhydrolin	10.70	72.4 µM	3.56E-13	NEO1	14	3.69E-27
8	SGTC_2689	tacrolimus	10.60	100.0 µM	6.44E-13		3	1.36E-26
9	SGTC_1666	st014829	10.20	29.0 µM	4.61E-12	calcium & mitochondrial duress	3	1.05E-24
10	SGTC_2873	9040596	10.10	71.4 µM	7.38E-12		2	2.96E-24
11	SGTC_2526	patulin	9.89	37.6 µM	1.83E-11		9	2.19E-23
12	SGTC_1581	himbacine	9.83	57.9 µM	2.48E-11	Golgi	8	4.28E-23
13	SGTC_2981	9013952	9.71	39.0 µM	4.22E-11		3	1.38E-22
14	SGTC_1665	st014639	8.79	11.4 µM	2.11E-9	NEO1	1	7.59E-19
15	SGTC_2036	5160441	8.69	105.0 µM	3.11E-9	NEO1	23	1.78E-18
16	SGTC_52	0329-0193	8.48	875.0 nM	7.17E-9	TSC3-RPN4	3	1.12E-17
17	SGTC_70	0141-0289	8.03	43.3 µM	3.98E-8	mitochondrial stress	41	4.80E-16
18	SGTC_3225	9130819	8.02	49.5 µM	4.18E-8	amide catabolism	2	5.35E-16
19	SGTC_232	clozapine	7.90	119.0 µM	6.52E-8	NEO1	20	1.42E-15
20	SGTC_1306	1185-0270	7.89	9.0 µM	6.61E-8	Golgi	19	1.46E-15

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.360	3.19E-103	YDR323C	PEP7	hom	Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.350	3.38E-97	YOR036W	PEP12	hom	Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.302	1.00E-71	YDR495C	VPS3	hom	Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.299	3.99E-70	YKR001C	VPS1	hom	Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
0.296	1.46E-68	YGL054C	ERV14	hom	COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
0.291	2.53E-66	YLR148W	PEP3	hom	Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
0.268	2.86E-56	YDR455C_d	YDR455C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.256	4.30E-51	YOL018C	TLG2	hom	Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
0.253	5.32E-50	YLR025W	SNF7	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.252	1.59E-49	YDL192W	ARF1	hom	ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.244	1.31E-46	YJR102C	VPS25	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.244	1.73E-46	YJL124C	LSM1	hom	Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.244	1.85E-46	YJL004C	SYS1	hom	Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.240	5.53E-45	YPL002C	SNF8	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
0.233	1.58E-42	YDR456W	NHX1	hom	Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion