YGL103W / RPL28

Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL103W / RPL28

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.45|P-value:8.70E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.46|P-value:8.52E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.56|P-value:5.70E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.46|P-value:8.54E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.81|P-value:2.78E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.70|P-value:3.97E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:3.44|P-value:9.03E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.07|P-value:1.12E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:5.08|P-value:1.99E-6 ID:SGTC_1493|Compound:4348-0038|FD-Score:-3.87|P-value:2.03E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.62|P-value:1.36E-5 ID:SGTC_2018|Compound:5133068|FD-Score:3.75|P-value:3.41E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.53|P-value:6.79E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.88|P-value:1.92E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.92|P-value:3.94E-6 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:5.72|P-value:1.04E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.75|P-value:6.96E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.42|P-value:9.68E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.24|P-value:9.71E-7 ID:SGTC_2785|Compound:7756463|FD-Score:-3.98|P-value:1.38E-4 ID:SGTC_2800|Compound:7887935|FD-Score:4.28|P-value:5.21E-5 ID:SGTC_2981|Compound:9013952|FD-Score:3.74|P-value:3.49E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.59|P-value:1.57E-5 ID:SGTC_3000|Compound:9075062|FD-Score:7.37|P-value:1.03E-11 ID:SGTC_15|Compound:0266-0086|FD-Score:3.45|P-value:8.70E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.46|P-value:8.52E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.56|P-value:5.70E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.46|P-value:8.54E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.81|P-value:2.78E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.70|P-value:3.97E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:3.44|P-value:9.03E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.07|P-value:1.12E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:5.08|P-value:1.99E-6 ID:SGTC_1493|Compound:4348-0038|FD-Score:-3.87|P-value:2.03E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.62|P-value:1.36E-5 ID:SGTC_2018|Compound:5133068|FD-Score:3.75|P-value:3.41E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.53|P-value:6.79E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.88|P-value:1.92E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.92|P-value:3.94E-6 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:5.72|P-value:1.04E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.75|P-value:6.96E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.42|P-value:9.68E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.24|P-value:9.71E-7 ID:SGTC_2785|Compound:7756463|FD-Score:-3.98|P-value:1.38E-4 ID:SGTC_2800|Compound:7887935|FD-Score:4.28|P-value:5.21E-5 ID:SGTC_2981|Compound:9013952|FD-Score:3.74|P-value:3.49E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.59|P-value:1.57E-5 ID:SGTC_3000|Compound:9075062|FD-Score:7.37|P-value:1.03E-11

Top fitness defect scores for YGL103W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3000 9075062 7.37 71.4 µM 1.03E-11 2 8.27E-14 2.14
2 SGTC_2562 3',7'-dimethoxyflavone 5.72 64.9 µM 1.04E-7 3 5.40E-9 0.40
3 SGTC_2572 astragaloside iv 5.24 100.0 µM 9.71E-7 1 7.96E-8 0.73
4 SGTC_1373 2887-0003 5.08 58.5 µM 1.99E-6 Golgi 5 1.88E-7 0.65
5 SGTC_2560 caryophyllene 4.92 100.0 µM 3.94E-6 3 4.28E-7 0.52
6 SGTC_1512 0259-0544 4.62 12.2 µM 1.36E-5 3 1.90E-6 0.59
7 SGTC_2993 9054257 4.59 71.4 µM 1.57E-5 fatty acid desaturase (OLE1) 24 2.24E-6 0.01
8 SGTC_2800 7887935 4.28 81.8 µM 5.21E-5 RPP1 & pyrimidine depletion 8 9.47E-6 0.22
9 SGTC_1371 2421-0008 4.07 32.7 µM 1.12E-4 mitochondrial processes 22 2.37E-5 0.04
10 SGTC_843 0310-0051 3.81 143.0 µM 2.78E-4 3 7.04E-5 0.41
11 SGTC_2018 5133068 3.75 143.0 µM 3.41E-4 10 9.00E-5 0.16
12 SGTC_2981 9013952 3.74 39.0 µM 3.49E-4 12 9.26E-5 0.20
13 SGTC_923 1900-2434 3.70 73.3 µM 3.97E-4 RNA processing & uracil transport 13 1.08E-4 0.06
14 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 3.53 100.0 µM 6.79E-4 11 2.05E-4 0.02
15 SGTC_134 0269-0018 3.46 6.9 µM 8.52E-4 24 2.69E-4 0.02
16 SGTC_338 4055-0136 3.46 114.0 µM 8.54E-4 3 2.70E-4 0.01
17 SGTC_15 0266-0086 3.45 27.1 µM 8.70E-4 endomembrane recycling 15 2.76E-4 0.03
18 SGTC_924 2046-0044 3.44 21.3 µM 9.03E-4 11 2.89E-4 0.00
19 SGTC_2569 tetrandrine 3.42 100.0 µM 9.68E-4 9 3.14E-4 0.10
20 SGTC_999 4022-0066 3.27 22.7 µM 0.00152 Golgi 23 5.40E-4 1.82E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 8.50E-13 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.120 2.84E-12 YML003W_p YML003W_p hom Putative protein of unknown function
0.101 4.86E-9 YHR048W_p YHK8_p hom Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles
0.099 1.03E-8 YDL235C YPD1 het Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
0.091 1.10E-7 YKR050W TRK2 hom Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
0.090 1.56E-7 YDL169C UGX2 hom Protein of unknown function, transcript accumulates in response to any combination of stress conditions
0.090 1.84E-7 YDR541C_p YDR541C_p hom Putative dihydrokaempferol 4-reductase
0.085 8.61E-7 YGR048W UFD1 het Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
0.082 1.94E-6 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.082 2.03E-6 YOR113W AZF1 hom Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
0.081 2.67E-6 YGL105W ARC1 hom Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress
0.081 2.73E-6 YOL116W MSN1 hom Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.079 4.12E-6 YPL240C HSP82 hom Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
0.078 5.62E-6 YJL062W-A COA3 hom Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly
0.078 6.12E-6 YKR081C RPF2 het Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles