YGL125W / MET13

Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL125W / MET13

Click on Significant Values for Screen Details ID:SGTC_170|Compound:4197-0004|FD-Score:3.58|P-value:2.34E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:3.27|P-value:7.22E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.52|P-value:2.94E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:-3.62|P-value:1.71E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.58|P-value:3.73E-6 ID:SGTC_270|Compound:tamoxifen|FD-Score:-4.42|P-value:6.31E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.23|P-value:8.03E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.41|P-value:3.73E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:-3.34|P-value:4.62E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:3.55|P-value:2.61E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:4.06|P-value:3.63E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:-3.32|P-value:5.01E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.27|P-value:5.95E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:-5.13|P-value:2.06E-7 ID:SGTC_1271|Compound:0832-2808|FD-Score:-6.07|P-value:1.05E-9 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.41|P-value:4.33E-4 ID:SGTC_1518|Compound:st038567|FD-Score:3.41|P-value:4.42E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.81|P-value:9.91E-5 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.96|P-value:4.44E-5 ID:SGTC_1793|Compound:st048256|FD-Score:-3.82|P-value:7.86E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.20|P-value:8.98E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.89|P-value:5.85E-5 ID:SGTC_2281|Compound:7943571|FD-Score:3.20|P-value:8.97E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.35|P-value:5.43E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.80|P-value:8.65E-5 ID:SGTC_2762|Compound:leflunomide|FD-Score:4.24|P-value:1.70E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-6.60|P-value:3.71E-11 ID:SGTC_3183|Compound:9108214|FD-Score:-3.21|P-value:7.35E-4 ID:SGTC_3336|Compound:9143619|FD-Score:-3.66|P-value:1.45E-4 ID:SGTC_3354|Compound:9151612|FD-Score:-3.71|P-value:1.18E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:3.58|P-value:2.34E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:3.27|P-value:7.22E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.52|P-value:2.94E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:-3.62|P-value:1.71E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.58|P-value:3.73E-6 ID:SGTC_270|Compound:tamoxifen|FD-Score:-4.42|P-value:6.31E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.23|P-value:8.03E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.41|P-value:3.73E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:-3.34|P-value:4.62E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:3.55|P-value:2.61E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:4.06|P-value:3.63E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:-3.32|P-value:5.01E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.27|P-value:5.95E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:-5.13|P-value:2.06E-7 ID:SGTC_1271|Compound:0832-2808|FD-Score:-6.07|P-value:1.05E-9 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.41|P-value:4.33E-4 ID:SGTC_1518|Compound:st038567|FD-Score:3.41|P-value:4.42E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.81|P-value:9.91E-5 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.96|P-value:4.44E-5 ID:SGTC_1793|Compound:st048256|FD-Score:-3.82|P-value:7.86E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.20|P-value:8.98E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.89|P-value:5.85E-5 ID:SGTC_2281|Compound:7943571|FD-Score:3.20|P-value:8.97E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.35|P-value:5.43E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-3.80|P-value:8.65E-5 ID:SGTC_2762|Compound:leflunomide|FD-Score:4.24|P-value:1.70E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-6.60|P-value:3.71E-11 ID:SGTC_3183|Compound:9108214|FD-Score:-3.21|P-value:7.35E-4 ID:SGTC_3336|Compound:9143619|FD-Score:-3.66|P-value:1.45E-4 ID:SGTC_3354|Compound:9151612|FD-Score:-3.71|P-value:1.18E-4

Top fitness defect scores for YGL125W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_260 nsc-65238 4.58 4.0 µM 3.73E-6 endomembrane recycling 7 2.30E-6
2 SGTC_2762 leflunomide 4.24 42.1 µM 1.70E-5 20 1.11E-5
3 SGTC_1018 4259-0013 4.06 28.1 µM 3.63E-5 12 2.45E-5
4 SGTC_1563 conessine 3.81 56.1 µM 9.91E-5 19 6.95E-5
5 SGTC_170 4197-0004 3.58 226.0 µM 2.34E-4 27 1.69E-4
6 SGTC_893 0958-0369 3.55 48.9 µM 2.61E-4 37 1.90E-4
7 SGTC_219 mitoxanthrone 3.52 164.4 µM 2.94E-4 DNA intercalators 62 2.15E-4
8 SGTC_1286 0929-0070 3.41 131.0 µM 4.33E-4 37 3.21E-4
9 SGTC_1518 st038567 3.41 90.8 µM 4.42E-4 28 3.28E-4
10 SGTC_2503 beta-caryophyllene alcohol 3.35 24.7 µM 5.43E-4 22 4.06E-4
11 SGTC_171 k064-0041 3.27 9.7 µM 7.22E-4 heme requiring 42 5.46E-4
12 SGTC_407 eburnamonine 3.23 100.0 µM 8.03E-4 40 6.11E-4
13 SGTC_2281 7943571 3.20 200.0 µM 8.97E-4 28 6.85E-4
14 SGTC_1919 st067113 3.20 52.3 µM 8.98E-4 TSC3-RPN4 64 6.85E-4
15 SGTC_1152 3909-8331 3.15 15.9 µM 0.00106 34 8.17E-4
16 SGTC_2777 climbazole 3.10 1.5 µM 0.00124 iron homeostasis 92 9.57E-4
17 SGTC_1782 st039423 3.05 80.6 µM 0.00146 70 0.00114
18 SGTC_2945 9067681 3.02 61.8 µM 0.00160 43 0.00125
19 SGTC_82 0054-0107 3.01 293.8 µM 0.00169 30 0.00132
20 SGTC_1046 2001-0062 2.97 216.0 µM 0.00188 53 0.00147

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 2.46E-16 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.129 7.45E-14 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.110 1.54E-10 YPR140W TAZ1 hom Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome
0.109 2.63E-10 YKL033W TTI1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
0.096 2.53E-8 YMR026C PEX12 hom C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
0.094 4.53E-8 YPR110C RPC40 het RNA polymerase subunit AC40, common to RNA polymerase I and III
0.090 1.70E-7 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.083 1.52E-6 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.081 2.51E-6 YDR465C RMT2 hom Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
0.080 3.14E-6 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.080 3.48E-6 YDR244W PEX5 hom Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
0.079 4.28E-6 YLR084C RAX2 hom N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
0.079 4.90E-6 YJR067C YAE1 het Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.076 1.11E-5 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.076 1.15E-5 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data