Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
Zygosity: Homozygous strain
fixedexpanded
![]() ![]() Click on Significant Values for Screen Details |
Top fitness defect scores for YGL127C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.163 | 2.78E-21 | YOR236W | DFR1 | het | Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism |
0.148 | 8.40E-18 | YHR041C | SRB2 | hom | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance |
0.145 | 3.06E-17 | YHR064C | SSZ1 | hom | Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP |
0.132 | 1.35E-14 | YDR443C | SSN2 | hom | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation |
0.132 | 2.08E-14 | YNL246W | VPS75 | hom | NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress |
0.127 | 1.34E-13 | YOR312C | RPL20B | hom | Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication |
0.126 | 2.63E-13 | YGR078C | PAC10 | hom | Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding |
0.125 | 4.08E-13 | YFL002C | SPB4 | het | Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients |
0.122 | 1.47E-12 | YHR205W | SCH9 | hom | AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan |
0.120 | 3.14E-12 | YKL176C | LST4 | hom | Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface |
0.119 | 5.30E-12 | YER165W | PAB1 | het | Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G |
0.113 | 4.97E-11 | YDR404C | RPB7 | het | RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation |
0.113 | 5.47E-11 | YKR097W | PCK1 | hom | Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol |
0.112 | 7.59E-11 | YML127W | RSC9 | het | Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway |
0.111 | 1.22E-10 | YJL081C | ARP4 | het | Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes |