YGL135W / RPL1B

Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL135W / RPL1B

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.61|P-value:9.63E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:3.43|P-value:2.07E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:5.54|P-value:3.46E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.48|P-value:6.19E-5 ID:SGTC_84|Compound:0443-0269|FD-Score:3.63|P-value:9.01E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:4.31|P-value:3.90E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:3.77|P-value:4.88E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:3.98|P-value:1.89E-5 ID:SGTC_446|Compound:go6976|FD-Score:-2.93|P-value:5.70E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.38|P-value:2.51E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:3.21|P-value:4.88E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:-4.81|P-value:7.16E-8 ID:SGTC_1136|Compound:pergolide|FD-Score:4.92|P-value:1.45E-7 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.42|P-value:2.10E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-2.93|P-value:5.60E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.15|P-value:6.01E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.53|P-value:1.33E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:4.04|P-value:1.42E-5 ID:SGTC_1635|Compound:st007035|FD-Score:4.41|P-value:2.27E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-7.40|P-value:5.15E-16 ID:SGTC_1779|Compound:st039427|FD-Score:3.09|P-value:7.44E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.04|P-value:3.81E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-2.89|P-value:6.61E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.31|P-value:1.27E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.92|P-value:8.23E-6 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.07|P-value:7.99E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.24|P-value:4.30E-4 ID:SGTC_1902|Compound:st060212|FD-Score:3.12|P-value:6.75E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-2.98|P-value:4.73E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.55|P-value:4.58E-5 ID:SGTC_2038|Compound:4100038|FD-Score:-2.81|P-value:9.01E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:4.86|P-value:2.04E-7 ID:SGTC_2173|Compound:5763948|FD-Score:3.23|P-value:4.47E-4 ID:SGTC_2177|Compound:5811945|FD-Score:-3.42|P-value:8.09E-5 ID:SGTC_2181|Compound:6316240|FD-Score:3.53|P-value:1.37E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.46|P-value:1.78E-4 ID:SGTC_2337|Compound:9009472|FD-Score:-3.27|P-value:1.52E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.20|P-value:5.01E-4 ID:SGTC_2485|Compound:5633444|FD-Score:3.25|P-value:4.19E-4 ID:SGTC_2511|Compound:berberine|FD-Score:3.59|P-value:1.07E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.67|P-value:2.68E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-2.83|P-value:8.16E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-2.99|P-value:4.59E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.40|P-value:2.29E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:4.22|P-value:6.10E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.61|P-value:3.47E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-3.25|P-value:1.60E-4 ID:SGTC_2837|Compound:9008476|FD-Score:-3.06|P-value:3.48E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.36|P-value:2.24E-12 ID:SGTC_3217|Compound:9129673|FD-Score:3.49|P-value:1.58E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.61|P-value:9.63E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:3.43|P-value:2.07E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:5.54|P-value:3.46E-9 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.48|P-value:6.19E-5 ID:SGTC_84|Compound:0443-0269|FD-Score:3.63|P-value:9.01E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:4.31|P-value:3.90E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:3.77|P-value:4.88E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:3.98|P-value:1.89E-5 ID:SGTC_446|Compound:go6976|FD-Score:-2.93|P-value:5.70E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.38|P-value:2.51E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:3.21|P-value:4.88E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:-4.81|P-value:7.16E-8 ID:SGTC_1136|Compound:pergolide|FD-Score:4.92|P-value:1.45E-7 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.42|P-value:2.10E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-2.93|P-value:5.60E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.15|P-value:6.01E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.53|P-value:1.33E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:4.04|P-value:1.42E-5 ID:SGTC_1635|Compound:st007035|FD-Score:4.41|P-value:2.27E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-7.40|P-value:5.15E-16 ID:SGTC_1779|Compound:st039427|FD-Score:3.09|P-value:7.44E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.04|P-value:3.81E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-2.89|P-value:6.61E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.31|P-value:1.27E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.92|P-value:8.23E-6 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.07|P-value:7.99E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.24|P-value:4.30E-4 ID:SGTC_1902|Compound:st060212|FD-Score:3.12|P-value:6.75E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-2.98|P-value:4.73E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.55|P-value:4.58E-5 ID:SGTC_2038|Compound:4100038|FD-Score:-2.81|P-value:9.01E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:4.86|P-value:2.04E-7 ID:SGTC_2173|Compound:5763948|FD-Score:3.23|P-value:4.47E-4 ID:SGTC_2177|Compound:5811945|FD-Score:-3.42|P-value:8.09E-5 ID:SGTC_2181|Compound:6316240|FD-Score:3.53|P-value:1.37E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.46|P-value:1.78E-4 ID:SGTC_2337|Compound:9009472|FD-Score:-3.27|P-value:1.52E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.20|P-value:5.01E-4 ID:SGTC_2485|Compound:5633444|FD-Score:3.25|P-value:4.19E-4 ID:SGTC_2511|Compound:berberine|FD-Score:3.59|P-value:1.07E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.67|P-value:2.68E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-2.83|P-value:8.16E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-2.99|P-value:4.59E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.40|P-value:2.29E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:4.22|P-value:6.10E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.61|P-value:3.47E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-3.25|P-value:1.60E-4 ID:SGTC_2837|Compound:9008476|FD-Score:-3.06|P-value:3.48E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.36|P-value:2.24E-12 ID:SGTC_3217|Compound:9129673|FD-Score:3.49|P-value:1.58E-4

Top fitness defect scores for YGL135W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_68 0986-0284 5.54 82.8 µM 3.46E-9 22 1.48E-8
2 SGTC_1136 pergolide 4.92 1.1 µM 1.45E-7 16 4.25E-7
3 SGTC_2170 morpholine 4.86 200.0 µM 2.04E-7 3 5.79E-7
4 SGTC_1635 st007035 4.41 42.4 µM 2.27E-6 190 5.10E-6
5 SGTC_118 0083-0097 4.31 135.3 µM 3.90E-6 translation 102 8.33E-6
6 SGTC_2775 cyproheptadine 4.22 69.6 µM 6.10E-6 13 1.25E-5
7 SGTC_1575 cyproterone acetate 4.04 48.0 µM 1.42E-5 16 2.68E-5
8 SGTC_369 0957-0393 3.98 4.5 µM 1.89E-5 cell wall signaling 24 3.48E-5
9 SGTC_236 1509-0011 3.77 5.3 µM 4.88E-5 395 8.23E-5
10 SGTC_84 0443-0269 3.63 128.8 µM 9.01E-5 31 1.44E-4
11 SGTC_58 0957-0393 3.61 9.0 µM 9.63E-5 cell wall 416 1.53E-4
12 SGTC_2511 berberine 3.59 10.5 µM 1.07E-4 60S ribosome export 29 1.68E-4
13 SGTC_1519 esterone 3.53 74.0 µM 1.33E-4 DNA intercalators 30 2.05E-4
14 SGTC_2181 6316240 3.53 54.3 µM 1.37E-4 33 2.10E-4
15 SGTC_3217 9129673 3.49 49.5 µM 1.58E-4 60 2.40E-4
16 SGTC_2232 chlorophetanol 3.46 41.5 µM 1.78E-4 TRP & mitochondrial translation 158 2.67E-4
17 SGTC_66 0109-0187 3.43 42.4 µM 2.07E-4 57 3.07E-4
18 SGTC_1223 0764-0573 3.42 147.0 µM 2.10E-4 41 3.11E-4
19 SGTC_2768 sulfasalazine 3.40 50.2 µM 2.29E-4 34 3.37E-4
20 SGTC_845 0330-0138 3.38 4.5 µM 2.51E-4 35 3.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.224 3.03E-39 YJL003W COX16 hom Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase
0.210 6.73E-35 YNL284C MRPL10 hom Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
0.208 5.34E-34 YPR116W RRG8 hom Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport
0.207 1.05E-33 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.206 1.89E-33 YDR237W MRPL7 hom Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
0.198 4.62E-31 YDL044C MTF2 hom Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
0.197 1.21E-30 YCR047C BUD23 hom Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
0.197 1.29E-30 YER077C_p YER077C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.193 1.39E-29 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.193 1.53E-29 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.193 1.67E-29 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.193 2.07E-29 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.190 1.36E-28 YGL107C RMD9 hom Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes
0.189 2.31E-28 YER122C GLO3 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p
0.188 4.28E-28 YNR037C RSM19 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein