YGL143C / MRF1

Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL143C / MRF1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:5.65|P-value:7.43E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.46|P-value:7.82E-5 ID:SGTC_59|Compound:0330-0160|FD-Score:4.82|P-value:2.10E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.70|P-value:8.88E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:9.07|P-value:4.42E-15 ID:SGTC_87|Compound:0100-0052|FD-Score:3.67|P-value:9.64E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:4.49|P-value:6.90E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:-4.72|P-value:1.81E-5 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.76|P-value:7.27E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:8.59|P-value:1.05E-13 ID:SGTC_275|Compound:trichlorophene|FD-Score:10.20|P-value:8.64E-19 ID:SGTC_310|Compound:1273-0058|FD-Score:9.51|P-value:2.07E-16 ID:SGTC_420|Compound:mitomycin C|FD-Score:4.64|P-value:4.06E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:3.71|P-value:8.59E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.68|P-value:6.07E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:4.47|P-value:7.56E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:5.82|P-value:3.64E-7 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:4.37|P-value:1.04E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:4.44|P-value:8.17E-5 ID:SGTC_1231|Compound:0242-0792|FD-Score:6.00|P-value:1.57E-7 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.56|P-value:8.65E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.61|P-value:7.47E-4 ID:SGTC_1624|Compound:st003710|FD-Score:4.05|P-value:2.99E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-5.92|P-value:1.19E-7 ID:SGTC_1683|Compound:st010134|FD-Score:6.39|P-value:2.58E-8 ID:SGTC_1742|Compound:st038235|FD-Score:6.14|P-value:8.41E-8 ID:SGTC_1990|Compound:st072050|FD-Score:4.03|P-value:3.23E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.16|P-value:5.64E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.66|P-value:3.85E-5 ID:SGTC_2446|Compound:5162541|FD-Score:4.51|P-value:6.39E-5 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:4.88|P-value:1.70E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.55|P-value:8.95E-4 ID:SGTC_2805|Compound:7950783|FD-Score:4.18|P-value:1.97E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.71|P-value:8.59E-4 ID:SGTC_3243|Compound:9134147|FD-Score:4.06|P-value:2.89E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:5.65|P-value:7.43E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.46|P-value:7.82E-5 ID:SGTC_59|Compound:0330-0160|FD-Score:4.82|P-value:2.10E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.70|P-value:8.88E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:9.07|P-value:4.42E-15 ID:SGTC_87|Compound:0100-0052|FD-Score:3.67|P-value:9.64E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:4.49|P-value:6.90E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:-4.72|P-value:1.81E-5 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.76|P-value:7.27E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:8.59|P-value:1.05E-13 ID:SGTC_275|Compound:trichlorophene|FD-Score:10.20|P-value:8.64E-19 ID:SGTC_310|Compound:1273-0058|FD-Score:9.51|P-value:2.07E-16 ID:SGTC_420|Compound:mitomycin C|FD-Score:4.64|P-value:4.06E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:3.71|P-value:8.59E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.68|P-value:6.07E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:4.47|P-value:7.56E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:5.82|P-value:3.64E-7 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:4.37|P-value:1.04E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:4.44|P-value:8.17E-5 ID:SGTC_1231|Compound:0242-0792|FD-Score:6.00|P-value:1.57E-7 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.56|P-value:8.65E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.61|P-value:7.47E-4 ID:SGTC_1624|Compound:st003710|FD-Score:4.05|P-value:2.99E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-5.92|P-value:1.19E-7 ID:SGTC_1683|Compound:st010134|FD-Score:6.39|P-value:2.58E-8 ID:SGTC_1742|Compound:st038235|FD-Score:6.14|P-value:8.41E-8 ID:SGTC_1990|Compound:st072050|FD-Score:4.03|P-value:3.23E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.16|P-value:5.64E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.66|P-value:3.85E-5 ID:SGTC_2446|Compound:5162541|FD-Score:4.51|P-value:6.39E-5 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:4.88|P-value:1.70E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.55|P-value:8.95E-4 ID:SGTC_2805|Compound:7950783|FD-Score:4.18|P-value:1.97E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.71|P-value:8.59E-4 ID:SGTC_3243|Compound:9134147|FD-Score:4.06|P-value:2.89E-4

Top fitness defect scores for YGL143C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_275 trichlorophene 10.20 5.7 µM 8.64E-19 mitochondrial stress 42 5.94E-25
2 SGTC_310 1273-0058 9.51 1.1 µM 2.07E-16 mitochondrial stress 51 9.75E-22
3 SGTC_70 0141-0289 9.07 43.3 µM 4.42E-15 mitochondrial stress 24 6.08E-20
4 SGTC_236 1509-0011 8.59 5.3 µM 1.05E-13 234 4.38E-18
5 SGTC_1683 st010134 6.39 710.0 nM 2.58E-8 3 8.38E-11
6 SGTC_1742 st038235 6.14 28.9 µM 8.41E-8 mitochondrial stress 42 4.13E-10
7 SGTC_1231 0242-0792 6.00 125.0 µM 1.57E-7 1 9.60E-10
8 SGTC_1036 k216-0215 5.82 33.5 µM 3.64E-7 mitochondrial response to ROS 15 2.99E-9
9 SGTC_10 0279-0212 5.65 42.6 µM 7.43E-7 TRP & mitochondrial translation 36 7.81E-9
10 SGTC_2232 chlorophetanol 5.16 41.5 µM 5.64E-6 TRP & mitochondrial translation 71 1.20E-7
11 SGTC_2664 lyso-paf C-16 4.88 73.5 µM 1.70E-5 5 5.33E-7
12 SGTC_59 0330-0160 4.82 1.3 µM 2.10E-5 66 7.06E-7
13 SGTC_2250 alverine citrate 4.66 130.0 µM 3.85E-5 111 1.60E-6
14 SGTC_420 mitomycin C 4.64 81.9 mM 4.06E-5 11 1.72E-6
15 SGTC_2446 5162541 4.51 200.0 µM 6.39E-5 6 3.17E-6
16 SGTC_134 0269-0018 4.49 6.9 µM 6.90E-5 113 3.52E-6
17 SGTC_661 1304-0007 4.47 33.6 µM 7.56E-5 14 3.97E-6
18 SGTC_28 pentachloropyridine N-oxide 4.46 1.6 µM 7.82E-5 TSC3-RPN4 49 4.16E-6
19 SGTC_1127 asiatic acid 4.44 681.4 nM 8.17E-5 14 4.41E-6
20 SGTC_1099 5-nonyloxytryptamine 4.37 2.9 µM 1.04E-4 13 6.10E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.412 1.32E-137 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.402 5.73E-130 YLR260W LCB5 hom Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules
0.397 7.17E-127 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.391 7.12E-123 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.388 4.04E-121 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.380 2.53E-115 YBR251W MRPS5 hom Mitochondrial ribosomal protein of the small subunit
0.379 9.06E-115 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit
0.376 8.94E-113 YPR166C MRP2 hom Mitochondrial ribosomal protein of the small subunit
0.376 2.90E-112 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.375 2.79E-112 YNR037C RSM19 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
0.370 1.43E-109 YDR079W PET100 hom Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
0.369 7.66E-109 YLR149C YLR149C hom Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
0.368 5.02E-108 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.367 3.07E-107 YDR237W MRPL7 hom Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
0.365 2.03E-106 YNL005C MRP7 hom Mitochondrial ribosomal protein of the large subunit