YGL145W / TIP20

Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL145W / TIP20

Click on Significant Values for Screen Details ID:SGTC_144|Compound:4161-2749|FD-Score:3.87|P-value:2.30E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:4.90|P-value:1.19E-7 ID:SGTC_181|Compound:1447-1644|FD-Score:3.32|P-value:2.43E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.42|P-value:1.59E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.55|P-value:9.32E-5 ID:SGTC_590|Compound:k048-0076|FD-Score:3.57|P-value:8.60E-5 ID:SGTC_595|Compound:3909-8003|FD-Score:4.28|P-value:3.22E-6 ID:SGTC_616|Compound:4092-0450|FD-Score:3.25|P-value:3.24E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.11|P-value:4.03E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.51|P-value:7.96E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.08|P-value:4.48E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.29|P-value:1.97E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:5.60|P-value:1.70E-9 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.07|P-value:4.75E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.89|P-value:2.10E-5 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.11|P-value:5.40E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-2.88|P-value:9.58E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.90|P-value:8.77E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.03|P-value:5.44E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-4.75|P-value:1.79E-7 ID:SGTC_1916|Compound:st067606|FD-Score:-5.52|P-value:1.64E-9 ID:SGTC_1938|Compound:st074717|FD-Score:-4.16|P-value:3.99E-6 ID:SGTC_1946|Compound:st076057|FD-Score:-2.88|P-value:9.41E-4 ID:SGTC_2025|Compound:5147754|FD-Score:-3.38|P-value:1.39E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.29|P-value:2.01E-4 ID:SGTC_2239|Compound:6685329|FD-Score:-3.73|P-value:3.05E-5 ID:SGTC_2294|Compound:7971431|FD-Score:4.85|P-value:1.51E-7 ID:SGTC_2495|Compound:cafestol|FD-Score:3.58|P-value:8.38E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:2.96|P-value:9.30E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.38|P-value:1.86E-4 ID:SGTC_2820|Compound:7989630|FD-Score:-3.03|P-value:5.45E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.03|P-value:7.32E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.18|P-value:4.14E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.10|P-value:4.22E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.10|P-value:5.68E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-2.96|P-value:7.17E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:3.87|P-value:2.30E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:4.90|P-value:1.19E-7 ID:SGTC_181|Compound:1447-1644|FD-Score:3.32|P-value:2.43E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.42|P-value:1.59E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.55|P-value:9.32E-5 ID:SGTC_590|Compound:k048-0076|FD-Score:3.57|P-value:8.60E-5 ID:SGTC_595|Compound:3909-8003|FD-Score:4.28|P-value:3.22E-6 ID:SGTC_616|Compound:4092-0450|FD-Score:3.25|P-value:3.24E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.11|P-value:4.03E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.51|P-value:7.96E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.08|P-value:4.48E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.29|P-value:1.97E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:5.60|P-value:1.70E-9 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.07|P-value:4.75E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.89|P-value:2.10E-5 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.11|P-value:5.40E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-2.88|P-value:9.58E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.90|P-value:8.77E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.03|P-value:5.44E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-4.75|P-value:1.79E-7 ID:SGTC_1916|Compound:st067606|FD-Score:-5.52|P-value:1.64E-9 ID:SGTC_1938|Compound:st074717|FD-Score:-4.16|P-value:3.99E-6 ID:SGTC_1946|Compound:st076057|FD-Score:-2.88|P-value:9.41E-4 ID:SGTC_2025|Compound:5147754|FD-Score:-3.38|P-value:1.39E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.29|P-value:2.01E-4 ID:SGTC_2239|Compound:6685329|FD-Score:-3.73|P-value:3.05E-5 ID:SGTC_2294|Compound:7971431|FD-Score:4.85|P-value:1.51E-7 ID:SGTC_2495|Compound:cafestol|FD-Score:3.58|P-value:8.38E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:2.96|P-value:9.30E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.38|P-value:1.86E-4 ID:SGTC_2820|Compound:7989630|FD-Score:-3.03|P-value:5.45E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.03|P-value:7.32E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.18|P-value:4.14E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.10|P-value:4.22E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.10|P-value:5.68E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-2.96|P-value:7.17E-4

Top fitness defect scores for YGL145W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_995 3931-2021 5.60 34.6 µM 1.70E-9 RPP1 & pyrimidine depletion 1 1.10E-8 0.59
2 SGTC_145 3737-0113 4.90 38.4 µM 1.19E-7 DNA intercalators 30 4.87E-7 0.09
3 SGTC_2294 7971431 4.85 200.0 µM 1.51E-7 3 6.02E-7 0.67
4 SGTC_595 3909-8003 4.28 73.7 µM 3.22E-6 4 9.33E-6 0.72
5 SGTC_1134 3477-0009 3.89 42.5 µM 2.10E-5 13 5.02E-5 0.03
6 SGTC_144 4161-2749 3.87 112.4 µM 2.30E-5 DNA intercalators 19 5.43E-5 0.18
7 SGTC_2495 cafestol 3.58 54.5 µM 8.38E-5 Golgi 13 1.74E-4 0.03
8 SGTC_590 k048-0076 3.57 22.4 µM 8.60E-5 RSC & ERG11 14 1.78E-4 0.01
9 SGTC_589 1227-0070 3.55 34.1 µM 9.32E-5 15 1.91E-4 0.01
10 SGTC_508 roscovitine 3.42 141.0 µM 1.59E-4 2 3.08E-4 0.24
11 SGTC_2813 7971799 3.38 71.4 µM 1.86E-4 15 3.56E-4 0.01
12 SGTC_181 1447-1644 3.32 8.1 µM 2.43E-4 endomembrane recycling 16 4.53E-4 0.02
13 SGTC_616 4092-0450 3.25 108.0 µM 3.24E-4 8 5.86E-4 0.21
14 SGTC_2913 7966556 3.18 36.6 µM 4.14E-4 60S ribosome export 13 7.30E-4 0.00
15 SGTC_1206 3978-0017 3.11 132.0 µM 5.40E-4 ERG2 17 9.28E-4 0.00
16 SGTC_2944 9067346 3.10 25.6 µM 5.68E-4 21 9.72E-4 0.03
17 SGTC_2857 9024691 3.03 58.4 µM 7.32E-4 11 0.00122 0.10
18 SGTC_2607 solasodine 2.96 47.4 µM 9.30E-4 32 0.00152 0.01
19 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 2.93 100.0 µM 0.00104 12 0.00167 0.04
20 SGTC_2962 9082311 2.92 13.2 µM 0.00109 26 0.00175 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.209 2.49E-34 YLR280C_d YLR280C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.167 2.32E-22 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.159 1.85E-20 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.153 4.84E-19 YEL040W UTR2 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
0.143 7.77E-17 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.143 9.76E-17 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.137 2.00E-15 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.135 4.62E-15 YGL245W GUS1 het Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
0.134 5.91E-15 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.134 6.63E-15 YML054C CYB2 hom Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
0.129 6.67E-14 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.121 2.22E-12 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.119 3.78E-12 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
0.117 8.80E-12 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition
0.111 1.39E-10 YHR020W YHR020W het Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments