YGL151W / NUT1

Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL151W / NUT1

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:5.54|P-value:4.50E-9 ID:SGTC_38|Compound:0929-0051|FD-Score:-4.31|P-value:3.54E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:5.58|P-value:3.52E-9 ID:SGTC_42|Compound:0133-0086|FD-Score:-5.14|P-value:4.28E-8 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.27|P-value:3.59E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.02|P-value:8.88E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:3.27|P-value:3.57E-4 ID:SGTC_389|Compound:tpck|FD-Score:3.10|P-value:6.62E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:7.84|P-value:2.02E-16 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.18|P-value:7.32E-6 ID:SGTC_591|Compound:4299-0174|FD-Score:3.13|P-value:6.05E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:4.10|P-value:1.10E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:4.65|P-value:7.26E-7 ID:SGTC_619|Compound:1652-1634|FD-Score:-3.12|P-value:5.62E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.71|P-value:5.37E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:3.07|P-value:7.48E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-4.21|P-value:5.64E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:-3.15|P-value:5.09E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.62|P-value:8.77E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:-3.06|P-value:6.92E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.80|P-value:4.11E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.32|P-value:2.93E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-2.99|P-value:8.99E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:6.62|P-value:3.26E-12 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.85|P-value:3.31E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.53|P-value:1.29E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.13|P-value:5.89E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.07|P-value:6.70E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.78|P-value:4.41E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.05|P-value:7.96E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:-3.96|P-value:1.81E-5 ID:SGTC_1712|Compound:st032288|FD-Score:10.60|P-value:1.20E-28 ID:SGTC_1959|Compound:st077091|FD-Score:4.68|P-value:6.00E-7 ID:SGTC_2021|Compound:4019055|FD-Score:5.06|P-value:7.88E-8 ID:SGTC_2024|Compound:5144227|FD-Score:-3.14|P-value:5.15E-4 ID:SGTC_2054|Compound:5233173|FD-Score:3.45|P-value:1.78E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.87|P-value:3.10E-5 ID:SGTC_2214|Compound:7179297|FD-Score:3.03|P-value:8.50E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.69|P-value:6.68E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.24|P-value:3.60E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.73|P-value:5.49E-5 ID:SGTC_2917|Compound:7979103|FD-Score:3.61|P-value:9.13E-5 ID:SGTC_2925|Compound:9010536|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.49|P-value:6.14E-9 ID:SGTC_2950|Compound:9072895|FD-Score:-4.17|P-value:6.89E-6 ID:SGTC_2989|Compound:9073649|FD-Score:3.84|P-value:3.42E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.83|P-value:3.15E-5 ID:SGTC_3160|Compound:9100216|FD-Score:3.04|P-value:8.35E-4 ID:SGTC_3165|Compound:9101544|FD-Score:7.88|P-value:1.46E-16 ID:SGTC_37|Compound:0566-0070|FD-Score:5.54|P-value:4.50E-9 ID:SGTC_38|Compound:0929-0051|FD-Score:-4.31|P-value:3.54E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:5.58|P-value:3.52E-9 ID:SGTC_42|Compound:0133-0086|FD-Score:-5.14|P-value:4.28E-8 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.27|P-value:3.59E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.02|P-value:8.88E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:3.27|P-value:3.57E-4 ID:SGTC_389|Compound:tpck|FD-Score:3.10|P-value:6.62E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:7.84|P-value:2.02E-16 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.18|P-value:7.32E-6 ID:SGTC_591|Compound:4299-0174|FD-Score:3.13|P-value:6.05E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:4.10|P-value:1.10E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:4.65|P-value:7.26E-7 ID:SGTC_619|Compound:1652-1634|FD-Score:-3.12|P-value:5.62E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.71|P-value:5.37E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:3.07|P-value:7.48E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-4.21|P-value:5.64E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:-3.15|P-value:5.09E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.62|P-value:8.77E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:-3.06|P-value:6.92E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.80|P-value:4.11E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.32|P-value:2.93E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-2.99|P-value:8.99E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:6.62|P-value:3.26E-12 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.85|P-value:3.31E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.53|P-value:1.29E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.13|P-value:5.89E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.07|P-value:6.70E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.78|P-value:4.41E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.05|P-value:7.96E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:-3.96|P-value:1.81E-5 ID:SGTC_1712|Compound:st032288|FD-Score:10.60|P-value:1.20E-28 ID:SGTC_1959|Compound:st077091|FD-Score:4.68|P-value:6.00E-7 ID:SGTC_2021|Compound:4019055|FD-Score:5.06|P-value:7.88E-8 ID:SGTC_2024|Compound:5144227|FD-Score:-3.14|P-value:5.15E-4 ID:SGTC_2054|Compound:5233173|FD-Score:3.45|P-value:1.78E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.87|P-value:3.10E-5 ID:SGTC_2214|Compound:7179297|FD-Score:3.03|P-value:8.50E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.69|P-value:6.68E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.24|P-value:3.60E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.73|P-value:5.49E-5 ID:SGTC_2917|Compound:7979103|FD-Score:3.61|P-value:9.13E-5 ID:SGTC_2925|Compound:9010536|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.49|P-value:6.14E-9 ID:SGTC_2950|Compound:9072895|FD-Score:-4.17|P-value:6.89E-6 ID:SGTC_2989|Compound:9073649|FD-Score:3.84|P-value:3.42E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.83|P-value:3.15E-5 ID:SGTC_3160|Compound:9100216|FD-Score:3.04|P-value:8.35E-4 ID:SGTC_3165|Compound:9101544|FD-Score:7.88|P-value:1.46E-16

Top fitness defect scores for YGL151W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1712 st032288 10.60 53.4 µM 1.20E-28 calcium & mitochondrial duress 20 1.04E-26
2 SGTC_3165 9101544 7.88 49.5 µM 1.46E-16 1 1.66E-15
3 SGTC_422 cantharidin 7.84 155.0 µM 2.02E-16 cell wall signaling 12 2.25E-15
4 SGTC_963 1269-2853 6.62 151.0 µM 3.26E-12 2 1.81E-11
5 SGTC_40 0327-0103 5.58 19.8 µM 3.52E-9 7 1.19E-8
6 SGTC_37 0566-0070 5.54 1.6 µM 4.50E-9 8 1.49E-8
7 SGTC_2947 9072639 5.49 7.7 µM 6.14E-9 tubulin folding & SWR complex 12 1.99E-8
8 SGTC_2021 4019055 5.06 173.0 µM 7.88E-8 endomembrane recycling 61 2.13E-7
9 SGTC_1959 st077091 4.68 55.8 µM 6.00E-7 RPP1 & pyrimidine depletion 28 1.41E-6
10 SGTC_609 0986-0026 4.65 86.3 µM 7.26E-7 calcium & mitochondrial duress 18 1.68E-6
11 SGTC_423 5-fluorouracil 4.18 457.0 µM 7.32E-6 exosome 17 1.44E-5
12 SGTC_600 1495-0418 4.10 41.6 µM 1.10E-5 RPP1 & pyrimidine depletion 7 2.10E-5
13 SGTC_2156 5649887 3.87 115.0 µM 3.10E-5 RPP1 & pyrimidine depletion 11 5.54E-5
14 SGTC_1019 4262-0212 3.85 3.4 µM 3.31E-5 ergosterol depletion effects on membrane 65 5.89E-5
15 SGTC_2989 9073649 3.84 71.4 µM 3.42E-5 44 6.06E-5
16 SGTC_903 1134-0042 3.80 114.0 µM 4.11E-5 23 7.20E-5
17 SGTC_1225 1216-0074 3.78 20.6 µM 4.41E-5 28 7.69E-5
18 SGTC_2896 9052656 3.73 71.4 µM 5.49E-5 endomembrane recycling 135 9.44E-5
19 SGTC_2507 purpurin 3.69 79.7 µM 6.68E-5 copper-dependent oxidative stress 50 1.13E-4
20 SGTC_829 1635-0120 3.62 117.0 µM 8.77E-5 DNA damage response 54 1.46E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.293 2.41E-67 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.231 5.65E-42 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.192 3.02E-29 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.181 3.11E-26 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.162 3.03E-21 YGR012W YGR012W hom Putative cysteine synthase, localized to the mitochondrial outer membrane
0.162 3.68E-21 YDR357C_p CNL1_p hom Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.162 4.46E-21 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.162 4.64E-21 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.161 5.28E-21 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.158 3.17E-20 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.158 3.34E-20 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.156 8.34E-20 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.149 4.56E-18 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
0.148 5.29E-18 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.148 7.62E-18 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication