HIPHOP chemogenomics database

YGL163C / RAD54

DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL163C / RAD54

Click on Significant Values for Screen Details

Top fitness defect scores for YGL163C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1707	st032323	11.30	6.3 µM	1.78E-23	DNA damage response	8	7.61E-30
2	SGTC_829	1635-0120	10.30	117.0 µM	7.19E-20	DNA damage response	5	3.05E-25
3	SGTC_2612	dictamnine	10.20	15.5 µM	2.40E-19	RNA pol III & RNase P/MRP	2	1.42E-24
4	SGTC_244	nsc-207895	9.78	1.3 µM	4.95E-18	DNA damage response	4	6.80E-23
5	SGTC_3247	9135149	8.59	49.5 µM	2.83E-14	DNA damage response	4	4.32E-18
6	SGTC_1718	roemerine	8.29	11.9 µM	2.13E-13	redox potentiating	7	5.70E-17
7	SGTC_2947	9072639	8.15	7.7 µM	5.26E-13	tubulin folding & SWR complex	8	1.82E-16
8	SGTC_1936	st074407	7.50	74.3 µM	3.04E-11	DNA damage response	4	3.27E-14
9	SGTC_1082	camptothecin	7.12	424.9 nM	2.68E-10	DNA damage response	15	5.32E-13
10	SGTC_3028	9087778	6.79	49.5 µM	1.72E-9		2	5.78E-12
11	SGTC_429	1112-0049	6.70	25.5 µM	2.73E-9	DNA damage response	10	1.04E-11
12	SGTC_1930	st074634	6.68	34.3 µM	3.11E-9	RNA pol III & RNase P/MRP	6	1.23E-11
13	SGTC_268	camptothecin	6.65	39.6 µM	3.47E-9	DNA damage response	22	1.42E-11
14	SGTC_1703	st026983	6.43	48.3 µM	1.10E-8		1	6.20E-11
15	SGTC_1079	idarubicin	6.42	2.4 µM	1.18E-8	DNA damage response	5	6.78E-11
16	SGTC_1532	4',5'-dihydroxyflavone	6.31	78.7 µM	2.04E-8	Golgi	8	1.38E-10
17	SGTC_267	4-Nitroquinoline-1-oxide (4-NQO)	6.15	44.4 nM	4.66E-8	DNA damage response	18	3.97E-10
18	SGTC_2671	danthron	5.82	100.0 µM	2.19E-7	DNA damage response	15	2.90E-9
19	SGTC_219	mitoxanthrone	5.65	164.4 µM	4.90E-7	DNA intercalators	20	8.14E-9
20	SGTC_558	k064-0027	5.61	21.3 µM	5.78E-7	DNA damage response	7	1.01E-8

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.589	1.50E-312	YDR076W	RAD55	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.561	2.55E-277	YDR004W	RAD57	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.522	2.34E-234	YDL059C	RAD59	hom	Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.415	4.13E-140	YBR098W	MMS4	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.409	2.88E-135	YDR386W	MUS81	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.392	1.84E-123	YGL175C	SAE2	hom	Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.363	3.83E-105	YBR099C_d	YBR099C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.329	1.17E-85	YJL092W	SRS2	hom	DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.326	7.90E-84	YOR191W	ULS1	hom	Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.307	5.39E-74	YER095W	RAD51	hom	Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.228	7.62E-41	YLR032W	RAD5	hom	DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.223	5.91E-39	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.219	1.03E-37	YJL047C	RTT101	hom	Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.202	3.34E-32	YDR369C	XRS2	hom	Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
0.193	1.20E-29	YGL087C	MMS2	hom	Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress