YGL171W / ROK1

RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL171W / ROK1

Click on Significant Values for Screen Details ID:SGTC_124|Compound:0136-0217|FD-Score:3.96|P-value:4.09E-5 ID:SGTC_170|Compound:4197-0004|FD-Score:3.13|P-value:9.20E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.65|P-value:1.90E-6 ID:SGTC_571|Compound:k831-0106|FD-Score:3.51|P-value:2.38E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.39|P-value:4.15E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:-3.27|P-value:6.30E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.65|P-value:1.42E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.45|P-value:3.02E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.42|P-value:3.33E-4 ID:SGTC_1630|Compound:st005209|FD-Score:4.30|P-value:9.70E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-5.55|P-value:2.07E-8 ID:SGTC_1689|Compound:st020265|FD-Score:-3.40|P-value:3.95E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.74|P-value:9.97E-5 ID:SGTC_1917|Compound:st066507|FD-Score:3.13|P-value:9.08E-4 ID:SGTC_1931|Compound:st074438|FD-Score:-3.61|P-value:1.86E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.91|P-value:5.62E-5 ID:SGTC_1967|Compound:st070177|FD-Score:3.49|P-value:2.56E-4 ID:SGTC_1995|Compound:st072814|FD-Score:-3.34|P-value:5.00E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.43|P-value:3.29E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.86|P-value:6.35E-5 ID:SGTC_2092|Compound:5303694|FD-Score:3.50|P-value:2.49E-4 ID:SGTC_2102|Compound:5377816|FD-Score:-3.35|P-value:4.86E-4 ID:SGTC_2159|Compound:5658611|FD-Score:6.33|P-value:1.69E-10 ID:SGTC_2260|Compound:7964312|FD-Score:3.14|P-value:8.92E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.78|P-value:8.50E-5 ID:SGTC_2313|Compound:7726885|FD-Score:-4.12|P-value:2.36E-5 ID:SGTC_2315|Compound:7733312|FD-Score:4.97|P-value:4.03E-7 ID:SGTC_2329|Compound:6138490|FD-Score:3.78|P-value:8.73E-5 ID:SGTC_2399|Compound:5884133|FD-Score:-3.92|P-value:5.49E-5 ID:SGTC_2404|Compound:5161160|FD-Score:3.14|P-value:9.07E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.67|P-value:1.31E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-3.73|P-value:1.17E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-4.26|P-value:1.29E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.79|P-value:9.91E-7 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.88|P-value:6.57E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-6.01|P-value:1.42E-9 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.14|P-value:9.04E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.12|P-value:9.64E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.05|P-value:2.83E-5 ID:SGTC_2828|Compound:7998861|FD-Score:3.35|P-value:4.34E-4 ID:SGTC_2850|Compound:9015429|FD-Score:8.19|P-value:2.27E-16 ID:SGTC_2963|Compound:9082507|FD-Score:5.18|P-value:1.35E-7 ID:SGTC_2981|Compound:9013952|FD-Score:3.85|P-value:6.61E-5 ID:SGTC_3073|Compound:9114972|FD-Score:3.80|P-value:7.94E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-3.66|P-value:1.55E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.88|P-value:5.68E-5 ID:SGTC_3129|Compound:9127257|FD-Score:3.44|P-value:3.07E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.58|P-value:1.86E-4 ID:SGTC_3182|Compound:9108849|FD-Score:3.15|P-value:8.59E-4 ID:SGTC_3217|Compound:9129673|FD-Score:5.30|P-value:7.22E-8 ID:SGTC_3246|Compound:9135460|FD-Score:-3.53|P-value:2.51E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-4.78|P-value:1.20E-6 ID:SGTC_3315|Compound:9136395|FD-Score:5.78|P-value:4.67E-9 ID:SGTC_124|Compound:0136-0217|FD-Score:3.96|P-value:4.09E-5 ID:SGTC_170|Compound:4197-0004|FD-Score:3.13|P-value:9.20E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.65|P-value:1.90E-6 ID:SGTC_571|Compound:k831-0106|FD-Score:3.51|P-value:2.38E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.39|P-value:4.15E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:-3.27|P-value:6.30E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.65|P-value:1.42E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.45|P-value:3.02E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.42|P-value:3.33E-4 ID:SGTC_1630|Compound:st005209|FD-Score:4.30|P-value:9.70E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-5.55|P-value:2.07E-8 ID:SGTC_1689|Compound:st020265|FD-Score:-3.40|P-value:3.95E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.74|P-value:9.97E-5 ID:SGTC_1917|Compound:st066507|FD-Score:3.13|P-value:9.08E-4 ID:SGTC_1931|Compound:st074438|FD-Score:-3.61|P-value:1.86E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.91|P-value:5.62E-5 ID:SGTC_1967|Compound:st070177|FD-Score:3.49|P-value:2.56E-4 ID:SGTC_1995|Compound:st072814|FD-Score:-3.34|P-value:5.00E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.43|P-value:3.29E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.86|P-value:6.35E-5 ID:SGTC_2092|Compound:5303694|FD-Score:3.50|P-value:2.49E-4 ID:SGTC_2102|Compound:5377816|FD-Score:-3.35|P-value:4.86E-4 ID:SGTC_2159|Compound:5658611|FD-Score:6.33|P-value:1.69E-10 ID:SGTC_2260|Compound:7964312|FD-Score:3.14|P-value:8.92E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.78|P-value:8.50E-5 ID:SGTC_2313|Compound:7726885|FD-Score:-4.12|P-value:2.36E-5 ID:SGTC_2315|Compound:7733312|FD-Score:4.97|P-value:4.03E-7 ID:SGTC_2329|Compound:6138490|FD-Score:3.78|P-value:8.73E-5 ID:SGTC_2399|Compound:5884133|FD-Score:-3.92|P-value:5.49E-5 ID:SGTC_2404|Compound:5161160|FD-Score:3.14|P-value:9.07E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.67|P-value:1.31E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-3.73|P-value:1.17E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-4.26|P-value:1.29E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.79|P-value:9.91E-7 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.88|P-value:6.57E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-6.01|P-value:1.42E-9 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.14|P-value:9.04E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.12|P-value:9.64E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.05|P-value:2.83E-5 ID:SGTC_2828|Compound:7998861|FD-Score:3.35|P-value:4.34E-4 ID:SGTC_2850|Compound:9015429|FD-Score:8.19|P-value:2.27E-16 ID:SGTC_2963|Compound:9082507|FD-Score:5.18|P-value:1.35E-7 ID:SGTC_2981|Compound:9013952|FD-Score:3.85|P-value:6.61E-5 ID:SGTC_3073|Compound:9114972|FD-Score:3.80|P-value:7.94E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-3.66|P-value:1.55E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.88|P-value:5.68E-5 ID:SGTC_3129|Compound:9127257|FD-Score:3.44|P-value:3.07E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.58|P-value:1.86E-4 ID:SGTC_3182|Compound:9108849|FD-Score:3.15|P-value:8.59E-4 ID:SGTC_3217|Compound:9129673|FD-Score:5.30|P-value:7.22E-8 ID:SGTC_3246|Compound:9135460|FD-Score:-3.53|P-value:2.51E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-4.78|P-value:1.20E-6 ID:SGTC_3315|Compound:9136395|FD-Score:5.78|P-value:4.67E-9

Top fitness defect scores for YGL171W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2850 9015429 8.19 58.4 µM 2.27E-16 1 1.33E-16 3.31
2 SGTC_2159 5658611 6.33 73.8 µM 1.69E-10 1 1.25E-10 1.17
3 SGTC_3315 9136395 5.78 68.5 µM 4.67E-9 3 3.65E-9 1.31
4 SGTC_3217 9129673 5.30 49.5 µM 7.22E-8 3 5.90E-8 0.18
5 SGTC_2963 9082507 5.18 9.0 µM 1.35E-7 3 1.12E-7 0.14
6 SGTC_2315 7733312 4.97 200.0 µM 4.03E-7 5 3.39E-7 0.70
7 SGTC_2604 4'-hydroxychalcone 4.79 11.3 µM 9.91E-7 7 8.45E-7 0.24
8 SGTC_184 1494-0393 4.65 24.7 µM 1.90E-6 21 1.64E-6 0.08
9 SGTC_1630 st005209 4.30 31.3 µM 9.70E-6 3 8.58E-6 0.63
10 SGTC_2804 7954240 4.05 19.5 µM 2.83E-5 10 2.54E-5 0.11
11 SGTC_124 0136-0217 3.96 7.1 µM 4.09E-5 mitochondrial stress 25 3.70E-5 0.06
12 SGTC_3126 9127922 3.88 49.5 µM 5.68E-5 fatty acid desaturase (OLE1) 32 5.16E-5 0.08
13 SGTC_2064 5220758 3.86 200.0 µM 6.35E-5 8 5.78E-5 0.04
14 SGTC_2981 9013952 3.85 39.0 µM 6.61E-5 9 6.03E-5 3.32E-4
15 SGTC_3073 9114972 3.80 49.5 µM 7.94E-5 60S ribosome export 5 7.25E-5 0.07
16 SGTC_2264 7948757 3.78 110.8 µM 8.50E-5 4 7.77E-5 0.62
17 SGTC_2329 6138490 3.78 200.0 µM 8.73E-5 13 7.99E-5 0.00
18 SGTC_1697 st025554 3.74 27.7 µM 9.97E-5 3 9.14E-5 0.38
19 SGTC_2510 benzyl isothiocyanate 3.67 8.3 µM 1.31E-4 27 1.21E-4 0.03
20 SGTC_1250 0485-0349 3.65 40.2 µM 1.42E-4 15 1.31E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.21E-10 YPR157W_p TDA6_p hom Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele
0.095 3.96E-8 YNL151C RPC31 het RNA polymerase III subunit C31
0.088 2.91E-7 YFL042C_p YFL042C_p hom Putative protein of unknown function; YFL042C is not an essential gene
0.083 1.31E-6 YDR062W LCB2 het Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.081 2.72E-6 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.080 3.50E-6 YBL074C AAR2 het Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
0.078 6.88E-6 YAR014C BUD14 hom Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
0.077 7.64E-6 YPR113W PIS1 het Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
0.077 8.47E-6 YGL210W YPT32 hom Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
0.076 9.20E-6 YMR065W KAR5 hom Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone
0.074 1.76E-5 YCR101C_p YCR101C_p hom Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
0.074 1.82E-5 YDR043C NRG1 hom Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication
0.073 2.25E-5 YOR032C HMS1 hom Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
0.073 2.50E-5 YHR195W NVJ1 hom Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
0.071 3.44E-5 YBL008W-A_p YBL008W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR