YGL175C / SAE2

Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL175C / SAE2

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:5.23|P-value:3.49E-8 ID:SGTC_11|Compound:0438-0307|FD-Score:3.55|P-value:1.19E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:3.09|P-value:6.80E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.83|P-value:5.34E-5 ID:SGTC_82|Compound:0054-0107|FD-Score:-6.59|P-value:1.08E-11 ID:SGTC_100|Compound:0086-0128|FD-Score:3.44|P-value:1.83E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.80|P-value:4.23E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:5.93|P-value:5.09E-10 ID:SGTC_184|Compound:1494-0393|FD-Score:7.12|P-value:1.16E-13 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.58|P-value:1.16E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.66|P-value:7.63E-7 ID:SGTC_244|Compound:nsc-207895|FD-Score:6.83|P-value:1.08E-12 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.16|P-value:8.48E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.09|P-value:6.68E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:8.44|P-value:2.05E-18 ID:SGTC_361|Compound:000s-0510|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.32|P-value:3.96E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.67|P-value:7.02E-7 ID:SGTC_385|Compound:1494-0393|FD-Score:3.94|P-value:2.28E-5 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.29|P-value:3.27E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.30|P-value:3.09E-4 ID:SGTC_493|Compound:zm226600|FD-Score:-5.37|P-value:2.50E-8 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-5.37|P-value:2.50E-8 ID:SGTC_558|Compound:k064-0027|FD-Score:6.54|P-value:8.20E-12 ID:SGTC_579|Compound:4491-1009|FD-Score:3.65|P-value:8.02E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:9.29|P-value:6.94E-22 ID:SGTC_993|Compound:3909-8757|FD-Score:3.33|P-value:2.80E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.05|P-value:1.40E-5 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.21|P-value:4.41E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:5.21|P-value:3.91E-8 ID:SGTC_1082|Compound:camptothecin|FD-Score:9.13|P-value:3.26E-21 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.53|P-value:1.80E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.23|P-value:6.28E-6 ID:SGTC_1373|Compound:2887-0003|FD-Score:2.99|P-value:9.53E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:-3.19|P-value:6.42E-4 ID:SGTC_1522|Compound:st012842|FD-Score:5.89|P-value:6.62E-10 ID:SGTC_1526|Compound:st051518|FD-Score:-3.47|P-value:2.32E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.76|P-value:4.94E-5 ID:SGTC_1615|Compound:st002656|FD-Score:4.09|P-value:1.19E-5 ID:SGTC_1632|Compound:st005650|FD-Score:-3.40|P-value:3.00E-4 ID:SGTC_1707|Compound:st032323|FD-Score:15.20|P-value:7.62E-55 ID:SGTC_1718|Compound:roemerine|FD-Score:4.88|P-value:2.44E-7 ID:SGTC_1759|Compound:st043059|FD-Score:3.34|P-value:2.72E-4 ID:SGTC_1863|Compound:st057641|FD-Score:3.42|P-value:1.99E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.07|P-value:7.33E-4 ID:SGTC_1898|Compound:st060199|FD-Score:4.22|P-value:6.53E-6 ID:SGTC_1913|Compound:st060213|FD-Score:3.01|P-value:9.10E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.42|P-value:2.56E-6 ID:SGTC_1930|Compound:st074634|FD-Score:4.19|P-value:7.38E-6 ID:SGTC_1999|Compound:st074831|FD-Score:-3.14|P-value:7.79E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.24|P-value:3.95E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.29|P-value:4.76E-6 ID:SGTC_2189|Compound:6047310|FD-Score:3.10|P-value:6.49E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.74|P-value:5.52E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.48|P-value:1.84E-6 ID:SGTC_2302|Compound:7771753|FD-Score:4.63|P-value:8.63E-7 ID:SGTC_2312|Compound:7706453|FD-Score:-3.27|P-value:4.83E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-5.95|P-value:7.68E-10 ID:SGTC_2412|Compound:st077135|FD-Score:3.03|P-value:8.45E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:2.99|P-value:9.79E-4 ID:SGTC_2463|Compound:5473429|FD-Score:5.67|P-value:2.57E-9 ID:SGTC_2464|Compound:5717415|FD-Score:3.15|P-value:5.42E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.08|P-value:7.12E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.09|P-value:1.17E-5 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.60|P-value:5.41E-12 ID:SGTC_2671|Compound:danthron|FD-Score:5.43|P-value:1.11E-8 ID:SGTC_2701|Compound:st077767|FD-Score:-4.70|P-value:9.43E-7 ID:SGTC_2814|Compound:7977482|FD-Score:3.31|P-value:3.00E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.86|P-value:3.35E-5 ID:SGTC_2827|Compound:7993847|FD-Score:3.00|P-value:9.34E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.07|P-value:9.65E-4 ID:SGTC_2932|Compound:9016711|FD-Score:4.67|P-value:7.22E-7 ID:SGTC_2935|Compound:9038016|FD-Score:3.04|P-value:8.22E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-3.79|P-value:6.44E-5 ID:SGTC_3114|Compound:9122766|FD-Score:3.26|P-value:3.63E-4 ID:SGTC_3247|Compound:9135149|FD-Score:8.55|P-value:7.39E-19 ID:SGTC_9|Compound:0092-0002|FD-Score:5.23|P-value:3.49E-8 ID:SGTC_11|Compound:0438-0307|FD-Score:3.55|P-value:1.19E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:3.09|P-value:6.80E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.83|P-value:5.34E-5 ID:SGTC_82|Compound:0054-0107|FD-Score:-6.59|P-value:1.08E-11 ID:SGTC_100|Compound:0086-0128|FD-Score:3.44|P-value:1.83E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.80|P-value:4.23E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:5.93|P-value:5.09E-10 ID:SGTC_184|Compound:1494-0393|FD-Score:7.12|P-value:1.16E-13 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.58|P-value:1.16E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.66|P-value:7.63E-7 ID:SGTC_244|Compound:nsc-207895|FD-Score:6.83|P-value:1.08E-12 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.16|P-value:8.48E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.09|P-value:6.68E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:8.44|P-value:2.05E-18 ID:SGTC_361|Compound:000s-0510|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.32|P-value:3.96E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:4.67|P-value:7.02E-7 ID:SGTC_385|Compound:1494-0393|FD-Score:3.94|P-value:2.28E-5 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.29|P-value:3.27E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.30|P-value:3.09E-4 ID:SGTC_493|Compound:zm226600|FD-Score:-5.37|P-value:2.50E-8 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-5.37|P-value:2.50E-8 ID:SGTC_558|Compound:k064-0027|FD-Score:6.54|P-value:8.20E-12 ID:SGTC_579|Compound:4491-1009|FD-Score:3.65|P-value:8.02E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:9.29|P-value:6.94E-22 ID:SGTC_993|Compound:3909-8757|FD-Score:3.33|P-value:2.80E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.05|P-value:1.40E-5 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.21|P-value:4.41E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:5.21|P-value:3.91E-8 ID:SGTC_1082|Compound:camptothecin|FD-Score:9.13|P-value:3.26E-21 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.53|P-value:1.80E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.23|P-value:6.28E-6 ID:SGTC_1373|Compound:2887-0003|FD-Score:2.99|P-value:9.53E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:-3.19|P-value:6.42E-4 ID:SGTC_1522|Compound:st012842|FD-Score:5.89|P-value:6.62E-10 ID:SGTC_1526|Compound:st051518|FD-Score:-3.47|P-value:2.32E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.76|P-value:4.94E-5 ID:SGTC_1615|Compound:st002656|FD-Score:4.09|P-value:1.19E-5 ID:SGTC_1632|Compound:st005650|FD-Score:-3.40|P-value:3.00E-4 ID:SGTC_1707|Compound:st032323|FD-Score:15.20|P-value:7.62E-55 ID:SGTC_1718|Compound:roemerine|FD-Score:4.88|P-value:2.44E-7 ID:SGTC_1759|Compound:st043059|FD-Score:3.34|P-value:2.72E-4 ID:SGTC_1863|Compound:st057641|FD-Score:3.42|P-value:1.99E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.07|P-value:7.33E-4 ID:SGTC_1898|Compound:st060199|FD-Score:4.22|P-value:6.53E-6 ID:SGTC_1913|Compound:st060213|FD-Score:3.01|P-value:9.10E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.42|P-value:2.56E-6 ID:SGTC_1930|Compound:st074634|FD-Score:4.19|P-value:7.38E-6 ID:SGTC_1999|Compound:st074831|FD-Score:-3.14|P-value:7.79E-4 ID:SGTC_2032|Compound:5185112|FD-Score:3.24|P-value:3.95E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.29|P-value:4.76E-6 ID:SGTC_2189|Compound:6047310|FD-Score:3.10|P-value:6.49E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.74|P-value:5.52E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.48|P-value:1.84E-6 ID:SGTC_2302|Compound:7771753|FD-Score:4.63|P-value:8.63E-7 ID:SGTC_2312|Compound:7706453|FD-Score:-3.27|P-value:4.83E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-5.95|P-value:7.68E-10 ID:SGTC_2412|Compound:st077135|FD-Score:3.03|P-value:8.45E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:2.99|P-value:9.79E-4 ID:SGTC_2463|Compound:5473429|FD-Score:5.67|P-value:2.57E-9 ID:SGTC_2464|Compound:5717415|FD-Score:3.15|P-value:5.42E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.08|P-value:7.12E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.09|P-value:1.17E-5 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.60|P-value:5.41E-12 ID:SGTC_2671|Compound:danthron|FD-Score:5.43|P-value:1.11E-8 ID:SGTC_2701|Compound:st077767|FD-Score:-4.70|P-value:9.43E-7 ID:SGTC_2814|Compound:7977482|FD-Score:3.31|P-value:3.00E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.86|P-value:3.35E-5 ID:SGTC_2827|Compound:7993847|FD-Score:3.00|P-value:9.34E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.07|P-value:9.65E-4 ID:SGTC_2932|Compound:9016711|FD-Score:4.67|P-value:7.22E-7 ID:SGTC_2935|Compound:9038016|FD-Score:3.04|P-value:8.22E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-3.79|P-value:6.44E-5 ID:SGTC_3114|Compound:9122766|FD-Score:3.26|P-value:3.63E-4 ID:SGTC_3247|Compound:9135149|FD-Score:8.55|P-value:7.39E-19

Top fitness defect scores for YGL175C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1707 st032323 15.20 6.3 µM 7.62E-55 DNA damage response 3 3.04E-52
2 SGTC_829 1635-0120 9.29 117.0 µM 6.94E-22 DNA damage response 7 7.82E-21
3 SGTC_1082 camptothecin 9.13 424.9 nM 3.26E-21 DNA damage response 6 3.41E-20
4 SGTC_3247 9135149 8.55 49.5 µM 7.39E-19 DNA damage response 5 5.97E-18
5 SGTC_268 camptothecin 8.44 39.6 µM 2.05E-18 DNA damage response 12 1.58E-17
6 SGTC_184 1494-0393 7.12 24.7 µM 1.16E-13 13 5.26E-13
7 SGTC_244 nsc-207895 6.83 1.3 µM 1.08E-12 DNA damage response 7 4.40E-12
8 SGTC_2638 epoxomicin 6.60 18.0 µM 5.41E-12 4 2.03E-11
9 SGTC_558 k064-0027 6.54 21.3 µM 8.20E-12 DNA damage response 5 3.02E-11
10 SGTC_174 3553-0389 5.93 74.3 µM 5.09E-10 5 1.53E-9
11 SGTC_1522 st012842 5.89 45.6 µM 6.62E-10 RNA pol III & RNase P/MRP 7 1.96E-9
12 SGTC_2463 5473429 5.67 200.0 µM 2.57E-9 ERG2 4 7.13E-9
13 SGTC_2671 danthron 5.43 100.0 µM 1.11E-8 DNA damage response 18 2.87E-8
14 SGTC_9 0092-0002 5.23 10.4 µM 3.49E-8 10 8.49E-8
15 SGTC_1079 idarubicin 5.21 2.4 µM 3.91E-8 DNA damage response 11 9.46E-8
16 SGTC_1718 roemerine 4.88 11.9 µM 2.44E-7 redox potentiating 24 5.39E-7
17 SGTC_381 3461-2219 4.67 42.7 µM 7.02E-7 copper-dependent oxidative stress 11 1.47E-6
18 SGTC_2932 9016711 4.67 44.5 µM 7.22E-7 29 1.51E-6
19 SGTC_238 5hr heat shock (37°C) + nsc-638432 4.66 8.9 µM 7.63E-7 heat shock/prefoldin 22 1.59E-6
20 SGTC_2302 7771753 4.63 200.0 µM 8.63E-7 25 1.79E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.536 8.01E-249 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.468 3.32E-182 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.423 7.24E-146 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.416 1.32E-140 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.400 6.90E-129 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.392 1.84E-123 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.381 2.24E-116 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.364 2.06E-105 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.329 1.21E-85 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.268 2.86E-56 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.264 1.40E-54 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.246 2.04E-47 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.243 2.49E-46 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.204 7.34E-33 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.179 1.64E-25 YNL273W TOF1 hom Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase