YGL179C / TOS3

Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL179C / TOS3

Click on Significant Values for Screen Details ID:SGTC_82|Compound:0054-0107|FD-Score:2.86|P-value:7.52E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:4.41|P-value:4.93E-7 ID:SGTC_114|Compound:0364-0001|FD-Score:2.95|P-value:5.18E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:2.81|P-value:9.09E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.43|P-value:4.31E-7 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.69|P-value:2.03E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:-2.87|P-value:7.12E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.20|P-value:1.92E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.59|P-value:3.36E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.34|P-value:1.03E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.58|P-value:3.44E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.26|P-value:1.50E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-4.47|P-value:3.41E-7 ID:SGTC_1144|Compound:3486-0183|FD-Score:-2.80|P-value:9.31E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.76|P-value:1.51E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.49|P-value:5.29E-5 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.69|P-value:9.62E-8 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.09|P-value:3.00E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.04|P-value:3.70E-6 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.18|P-value:2.08E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.18|P-value:1.77E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:-2.81|P-value:9.04E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:2.98|P-value:4.65E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:2.79|P-value:9.83E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:5.84|P-value:4.33E-11 ID:SGTC_1606|Compound:st000094|FD-Score:4.06|P-value:3.29E-6 ID:SGTC_1617|Compound:st002906|FD-Score:3.37|P-value:8.98E-5 ID:SGTC_1829|Compound:berberine|FD-Score:3.25|P-value:1.55E-4 ID:SGTC_1835|Compound:st008666|FD-Score:4.29|P-value:9.31E-7 ID:SGTC_1896|Compound:st058441|FD-Score:3.05|P-value:3.56E-4 ID:SGTC_1917|Compound:st066507|FD-Score:2.87|P-value:7.07E-4 ID:SGTC_1942|Compound:st076330|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-3.11|P-value:2.77E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.66|P-value:2.38E-5 ID:SGTC_1966|Compound:st070145|FD-Score:4.40|P-value:5.15E-7 ID:SGTC_2085|Compound:5307070|FD-Score:2.81|P-value:9.04E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.02|P-value:3.99E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.10|P-value:2.90E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:2.84|P-value:8.03E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:2.94|P-value:5.46E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-2.83|P-value:8.36E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:3.82|P-value:1.12E-5 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.38|P-value:8.90E-5 ID:SGTC_2671|Compound:danthron|FD-Score:2.80|P-value:9.33E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:2.89|P-value:6.62E-4 ID:SGTC_2907|Compound:7721985|FD-Score:3.00|P-value:4.39E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.02|P-value:3.97E-4 ID:SGTC_2958|Compound:9077254|FD-Score:2.95|P-value:5.23E-4 ID:SGTC_3031|Compound:9088762|FD-Score:-2.98|P-value:4.63E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.29|P-value:1.27E-4 ID:SGTC_3223|Compound:9129970|FD-Score:-4.23|P-value:1.29E-6 ID:SGTC_3264|Compound:9137442|FD-Score:3.13|P-value:2.58E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.69|P-value:2.10E-5 ID:SGTC_82|Compound:0054-0107|FD-Score:2.86|P-value:7.52E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:4.41|P-value:4.93E-7 ID:SGTC_114|Compound:0364-0001|FD-Score:2.95|P-value:5.18E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:2.81|P-value:9.09E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.43|P-value:4.31E-7 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.69|P-value:2.03E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:-2.87|P-value:7.12E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.20|P-value:1.92E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.59|P-value:3.36E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.34|P-value:1.03E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.58|P-value:3.44E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.26|P-value:1.50E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-4.47|P-value:3.41E-7 ID:SGTC_1144|Compound:3486-0183|FD-Score:-2.80|P-value:9.31E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.76|P-value:1.51E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.49|P-value:5.29E-5 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.69|P-value:9.62E-8 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.09|P-value:3.00E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.04|P-value:3.70E-6 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.18|P-value:2.08E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.18|P-value:1.77E-6 ID:SGTC_1479|Compound:0549-0158|FD-Score:-2.81|P-value:9.04E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:2.98|P-value:4.65E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:2.79|P-value:9.83E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:5.84|P-value:4.33E-11 ID:SGTC_1606|Compound:st000094|FD-Score:4.06|P-value:3.29E-6 ID:SGTC_1617|Compound:st002906|FD-Score:3.37|P-value:8.98E-5 ID:SGTC_1829|Compound:berberine|FD-Score:3.25|P-value:1.55E-4 ID:SGTC_1835|Compound:st008666|FD-Score:4.29|P-value:9.31E-7 ID:SGTC_1896|Compound:st058441|FD-Score:3.05|P-value:3.56E-4 ID:SGTC_1917|Compound:st066507|FD-Score:2.87|P-value:7.07E-4 ID:SGTC_1942|Compound:st076330|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-3.11|P-value:2.77E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.66|P-value:2.38E-5 ID:SGTC_1966|Compound:st070145|FD-Score:4.40|P-value:5.15E-7 ID:SGTC_2085|Compound:5307070|FD-Score:2.81|P-value:9.04E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.02|P-value:3.99E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.10|P-value:2.90E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:2.84|P-value:8.03E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:2.94|P-value:5.46E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-2.83|P-value:8.36E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:3.82|P-value:1.12E-5 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.38|P-value:8.90E-5 ID:SGTC_2671|Compound:danthron|FD-Score:2.80|P-value:9.33E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:2.89|P-value:6.62E-4 ID:SGTC_2907|Compound:7721985|FD-Score:3.00|P-value:4.39E-4 ID:SGTC_2921|Compound:7974240|FD-Score:3.02|P-value:3.97E-4 ID:SGTC_2958|Compound:9077254|FD-Score:2.95|P-value:5.23E-4 ID:SGTC_3031|Compound:9088762|FD-Score:-2.98|P-value:4.63E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.29|P-value:1.27E-4 ID:SGTC_3223|Compound:9129970|FD-Score:-4.23|P-value:1.29E-6 ID:SGTC_3264|Compound:9137442|FD-Score:3.13|P-value:2.58E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.69|P-value:2.10E-5

Top fitness defect scores for YGL179C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1525 8-methoxypsoralen 5.84 92.5 µM 4.33E-11 DNA damage response 11 2.56E-9
2 SGTC_1283 0922-0125 4.69 16.4 µM 9.62E-8 10 1.37E-6
3 SGTC_260 nsc-65238 4.43 4.0 µM 4.31E-7 endomembrane recycling 9 4.68E-6
4 SGTC_113 0099-0221 4.41 2.5 µM 4.93E-7 15 5.23E-6
5 SGTC_1966 st070145 4.40 16.1 µM 5.15E-7 18 5.42E-6
6 SGTC_1835 st008666 4.29 49.2 µM 9.31E-7 19 8.79E-6
7 SGTC_1468 k284-1086 4.18 134.0 µM 1.77E-6 DNA intercalators 28 1.48E-5
8 SGTC_1606 st000094 4.06 49.2 µM 3.29E-6 60S ribosome export 14 2.47E-5
9 SGTC_1317 1273-0028 4.04 910.0 nM 3.70E-6 azole & statin 48 2.72E-5
10 SGTC_2641 levomentholum 3.82 100.0 µM 1.12E-5 9 6.73E-5
11 SGTC_1146 3579-1023 3.76 7.0 µM 1.51E-5 20 8.60E-5
12 SGTC_3273 9137859 3.69 49.5 µM 2.10E-5 8 1.13E-4
13 SGTC_627 1391-0720 3.59 175.0 µM 3.36E-5 21 1.66E-4
14 SGTC_1023 4482-3888 3.58 16.0 µM 3.44E-5 TSC3-RPN4 28 1.69E-4
15 SGTC_1282 0886-0010 3.49 32.4 µM 5.29E-5 endomembrane recycling 47 2.40E-4
16 SGTC_2660 2',4'-dihydroxychalcone 3.38 17.1 µM 8.90E-5 35 3.68E-4
17 SGTC_1617 st002906 3.37 78.0 µM 8.98E-5 42 3.71E-4
18 SGTC_783 0848-0141 3.34 92.7 µM 1.03E-4 RSC & ERG11 26 4.15E-4
19 SGTC_3217 9129673 3.29 49.5 µM 1.27E-4 69 4.94E-4
20 SGTC_1082 camptothecin 3.26 424.9 nM 1.50E-4 DNA damage response 72 5.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 5.38E-14 YBR215W HPC2 hom Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes
0.108 3.57E-10 YJL216C IMA5 hom Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose
0.094 4.52E-8 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.091 1.43E-7 YDR490C PKH1 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p
0.090 1.83E-7 YPR019W MCM4 het Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
0.088 3.34E-7 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.088 3.84E-7 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.085 8.55E-7 YFL042C_p YFL042C_p hom Putative protein of unknown function; YFL042C is not an essential gene
0.083 1.72E-6 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.078 5.35E-6 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.074 1.65E-5 YLR359W ADE13 het Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
0.072 2.90E-5 YNL305C BXI1 hom Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
0.071 3.51E-5 YLR407W_p YLR407W_p hom Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
0.070 5.24E-5 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.069 5.70E-5 YOR095C RKI1 het Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis