HIPHOP chemogenomics database

YGL186C / TPN1

Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL186C / TPN1

Click on Significant Values for Screen Details

Top fitness defect scores for YGL186C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_718	1130-0073	14.50	349.0 µM	3.68E-20		1	1.08E-47
2	SGTC_2938	9059746	12.30	27.3 µM	3.77E-15	fatty acid desaturase (OLE1)	3	2.76E-35
3	SGTC_937	3122-0005	11.90	46.7 µM	3.54E-14		1	7.03E-33
4	SGTC_2330	7979373	10.70	200.0 µM	7.08E-12	60S ribosome export	2	3.41E-27
5	SGTC_1130	3454-2858	9.88	15.9 µM	2.65E-10	NEO1	16	2.60E-23
6	SGTC_197	4544-0043	9.42	41.6 µM	1.62E-9	endomembrane recycling	6	2.26E-21
7	SGTC_2821	7996764	9.16	71.4 µM	4.36E-9		1	2.60E-20
8	SGTC_2534	helenine	9.00	20.1 µM	7.85E-9	RSC complex & mRNA processing	2	1.11E-19
9	SGTC_526	4469-0568	8.93	170.0 µM	1.02E-8	azole & statin	7	2.13E-19
10	SGTC_3218	9131285	8.62	49.5 µM	3.09E-8	endomembrane recycling	6	3.23E-18
11	SGTC_2959	9079155	8.54	11.0 µM	4.20E-8		2	6.87E-18
12	SGTC_1969	st069479	8.14	40.1 µM	1.64E-7		1	1.97E-16
13	SGTC_3277	9138724	7.89	49.5 µM	3.76E-7	Golgi	5	1.50E-15
14	SGTC_625	k213-0058	7.84	121.0 µM	4.42E-7	translation	12	2.24E-15
15	SGTC_712	r015-0009	7.69	80.6 µM	7.22E-7		4	7.48E-15
16	SGTC_61	0978-8327	7.48	98.6 µM	1.39E-6	ergosterol biosynthesis	1	3.70E-14
17	SGTC_301	4119-0100	7.48	24.5 µM	1.39E-6	NEO1	24	3.70E-14
18	SGTC_3050	9093513	7.45	49.5 µM	1.53E-6		3	4.73E-14
19	SGTC_1466	k213-0079	7.41	57.5 µM	1.75E-6		2	6.56E-14
20	SGTC_2332	7982454	7.40	138.9 µM	1.77E-6		6	6.75E-14

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.165	6.18E-22	YDL118W_d	YDL118W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.134	5.89E-15	YMR202W	ERG2	hom	C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
0.127	1.64E-13	YDL119C_p	YDL119C_p	hom	Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
0.124	6.09E-13	YEL029C	BUD16	hom	Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
0.118	7.11E-12	YMR154C	RIM13	hom	Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.117	9.33E-12	YJL204C	RCY1	hom	F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.117	1.33E-11	YOR293W	RPS10A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.116	1.46E-11	YCR094W	CDC50	hom	Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
0.116	1.69E-11	YKL212W	SAC1	hom	Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
0.115	2.45E-11	YGR122W	YGR122W	hom	Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.115	2.94E-11	YGL054C	ERV14	hom	COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
0.114	4.25E-11	YLR025W	SNF7	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.113	5.22E-11	YOR070C	GYP1	hom	Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.111	1.09E-10	YMR063W	RIM9	hom	Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
0.105	9.53E-10	YMR077C	VPS20	hom	Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes