HIPHOP chemogenomics database

YGL200C / EMP24

Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL200C / EMP24

Click on Significant Values for Screen Details

Top fitness defect scores for YGL200C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_145	3737-0113	7.55	38.4 µM	1.59E-14	DNA intercalators	11	2.15E-14
2	SGTC_2666	tannic acid	7.54	100.0 µM	1.77E-14	iron homeostasis	5	2.39E-14
3	SGTC_3040	9091894	7.53	49.5 µM	1.87E-14	DNA intercalators	3	2.53E-14
4	SGTC_264	promethazine	6.13	12.5 mM	3.83E-10	DNA intercalators	18	4.51E-10
5	SGTC_17	0960-0276	6.03	49.8 µM	7.17E-10		4	8.38E-10
6	SGTC_2643	anethole	5.87	100.0 µM	1.90E-9		3	2.19E-9
7	SGTC_1290	0986-0249	5.28	10.2 µM	5.79E-8	DNA intercalators	7	6.40E-8
8	SGTC_2448	5185112	5.27	27.2 µM	6.17E-8		5	6.81E-8
9	SGTC_77	0828-0286	5.20	12.5 µM	8.93E-8	TSC3-RPN4	9	9.81E-8
10	SGTC_471	1-acyl-paf	5.02	4.0 µM	2.33E-7	DNA intercalators	13	2.53E-7
11	SGTC_144	4161-2749	5.02	112.4 µM	2.34E-7	DNA intercalators	10	2.54E-7
12	SGTC_3094	9117742	4.29	49.5 µM	8.48E-6		14	8.83E-6
13	SGTC_9	0092-0002	4.29	10.4 µM	8.72E-6		22	9.09E-6
14	SGTC_407	eburnamonine	4.20	100.0 µM	1.29E-5		9	1.34E-5
15	SGTC_470	aa-861	4.16	153.0 µM	1.55E-5	sphingolipid biosynthesis & PDR1	47	1.60E-5
16	SGTC_867	0518-0118	4.14	155.0 µM	1.68E-5	DNA intercalators	17	1.74E-5
17	SGTC_1415	3966-0071	4.13	78.1 µM	1.77E-5	DNA intercalators	9	1.83E-5
18	SGTC_330	1488-0319	4.13	80.1 µM	1.79E-5	RPP1 & pyrimidine depletion	8	1.85E-5
19	SGTC_1060	3448-7350	4.09	82.0 µM	2.07E-5	Golgi	32	2.14E-5
20	SGTC_85	0868-0259	3.77	160.1 µM	7.88E-5	60S ribosome export	12	8.01E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.413	3.29E-138	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.392	4.86E-123	YMR166C_p	YMR166C_p	hom	Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.366	9.02E-107	YOR133W	EFT1	hom	Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.364	1.78E-105	YDL223C	HBT1	hom	Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.360	3.22E-103	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.358	6.52E-102	YMR289W	ABZ2	hom	Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.332	9.00E-87	YPL157W	TGS1	hom	Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.321	3.96E-81	YDL058W	USO1	het	Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.306	1.41E-73	YGR065C	VHT1	het	High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.304	1.37E-72	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.297	2.34E-69	YHR172W	SPC97	het	Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.285	1.87E-63	YJL025W	RRN7	het	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.282	2.11E-62	YGR156W	PTI1	het	Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.257	1.71E-51	YDR458C	HEH2	hom	Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
0.253	3.16E-50	YMR208W	ERG12	het	Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate