YGL211W / NCS6

Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL211W / NCS6

Click on Significant Values for Screen Details ID:SGTC_91|Compound:0417-1751|FD-Score:3.24|P-value:8.37E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.85|P-value:1.02E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.90|P-value:8.33E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.80|P-value:1.24E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.52|P-value:3.18E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:-4.03|P-value:4.95E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:-4.64|P-value:3.81E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.64|P-value:2.22E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.02|P-value:5.31E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:3.38|P-value:5.16E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.62|P-value:2.19E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.56|P-value:2.76E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.83|P-value:1.42E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:3.88|P-value:8.11E-5 ID:SGTC_1005|Compound:4092-0690|FD-Score:-3.35|P-value:6.06E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:-5.26|P-value:1.86E-7 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.52|P-value:6.46E-6 ID:SGTC_1070|Compound:indatraline|FD-Score:3.44|P-value:4.19E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.67|P-value:1.80E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.50|P-value:3.35E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:3.80|P-value:1.14E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.08|P-value:3.69E-5 ID:SGTC_1652|Compound:st011932|FD-Score:-3.84|P-value:1.05E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-3.72|P-value:1.67E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.82|P-value:1.65E-6 ID:SGTC_1750|Compound:st081859|FD-Score:-3.73|P-value:1.60E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:15.70|P-value:1.22E-52 ID:SGTC_2029|Compound:5175081|FD-Score:4.48|P-value:6.80E-6 ID:SGTC_2046|Compound:4110841|FD-Score:-3.33|P-value:6.51E-4 ID:SGTC_2168|Compound:5727979|FD-Score:3.61|P-value:2.28E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.74|P-value:1.40E-4 ID:SGTC_2243|Compound:6634756|FD-Score:5.29|P-value:1.47E-7 ID:SGTC_2247|Compound:cerulenin|FD-Score:4.21|P-value:2.23E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.67|P-value:2.00E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.66|P-value:2.05E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.90|P-value:8.26E-5 ID:SGTC_2344|Compound:7990960|FD-Score:5.25|P-value:1.81E-7 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.42|P-value:4.50E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-3.69|P-value:1.88E-4 ID:SGTC_2470|Compound:5664817|FD-Score:4.92|P-value:8.99E-7 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:6.49|P-value:1.51E-10 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.86|P-value:8.91E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:9.84|P-value:7.41E-22 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.79|P-value:2.15E-14 ID:SGTC_2730|Compound:imipramine|FD-Score:3.33|P-value:6.03E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.86|P-value:9.07E-5 ID:SGTC_2788|Compound:5282734|FD-Score:4.22|P-value:2.11E-5 ID:SGTC_2820|Compound:7989630|FD-Score:-3.92|P-value:7.84E-5 ID:SGTC_2821|Compound:7996764|FD-Score:4.61|P-value:3.83E-6 ID:SGTC_2834|Compound:9001755|FD-Score:4.09|P-value:3.60E-5 ID:SGTC_2870|Compound:9038528|FD-Score:4.89|P-value:1.06E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-4.76|P-value:2.15E-6 ID:SGTC_2917|Compound:7979103|FD-Score:7.21|P-value:1.39E-12 ID:SGTC_2921|Compound:7974240|FD-Score:4.62|P-value:3.68E-6 ID:SGTC_2923|Compound:7992654|FD-Score:5.80|P-value:9.07E-9 ID:SGTC_2926|Compound:9010726|FD-Score:5.06|P-value:4.52E-7 ID:SGTC_3018|Compound:9082602|FD-Score:-5.99|P-value:3.54E-9 ID:SGTC_3037|Compound:9091026|FD-Score:-5.02|P-value:6.28E-7 ID:SGTC_3167|Compound:9103034|FD-Score:3.23|P-value:8.46E-4 ID:SGTC_3186|Compound:9110464|FD-Score:-3.38|P-value:5.63E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.53|P-value:3.30E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.89|P-value:8.81E-5 ID:SGTC_3274|Compound:9138298|FD-Score:3.88|P-value:8.20E-5 ID:SGTC_3330|Compound:9141424|FD-Score:3.97|P-value:5.82E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:3.24|P-value:8.37E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.85|P-value:1.02E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.90|P-value:8.33E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.80|P-value:1.24E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.52|P-value:3.18E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:-4.03|P-value:4.95E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:-4.64|P-value:3.81E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.64|P-value:2.22E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.02|P-value:5.31E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:3.38|P-value:5.16E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.62|P-value:2.19E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:3.56|P-value:2.76E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.83|P-value:1.42E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:3.88|P-value:8.11E-5 ID:SGTC_1005|Compound:4092-0690|FD-Score:-3.35|P-value:6.06E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:-5.26|P-value:1.86E-7 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.52|P-value:6.46E-6 ID:SGTC_1070|Compound:indatraline|FD-Score:3.44|P-value:4.19E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.67|P-value:1.80E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.50|P-value:3.35E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:3.80|P-value:1.14E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:4.08|P-value:3.69E-5 ID:SGTC_1652|Compound:st011932|FD-Score:-3.84|P-value:1.05E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-3.72|P-value:1.67E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.82|P-value:1.65E-6 ID:SGTC_1750|Compound:st081859|FD-Score:-3.73|P-value:1.60E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:15.70|P-value:1.22E-52 ID:SGTC_2029|Compound:5175081|FD-Score:4.48|P-value:6.80E-6 ID:SGTC_2046|Compound:4110841|FD-Score:-3.33|P-value:6.51E-4 ID:SGTC_2168|Compound:5727979|FD-Score:3.61|P-value:2.28E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.74|P-value:1.40E-4 ID:SGTC_2243|Compound:6634756|FD-Score:5.29|P-value:1.47E-7 ID:SGTC_2247|Compound:cerulenin|FD-Score:4.21|P-value:2.23E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.67|P-value:2.00E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.66|P-value:2.05E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.90|P-value:8.26E-5 ID:SGTC_2344|Compound:7990960|FD-Score:5.25|P-value:1.81E-7 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.42|P-value:4.50E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-3.69|P-value:1.88E-4 ID:SGTC_2470|Compound:5664817|FD-Score:4.92|P-value:8.99E-7 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:6.49|P-value:1.51E-10 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.86|P-value:8.91E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:9.84|P-value:7.41E-22 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.79|P-value:2.15E-14 ID:SGTC_2730|Compound:imipramine|FD-Score:3.33|P-value:6.03E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.86|P-value:9.07E-5 ID:SGTC_2788|Compound:5282734|FD-Score:4.22|P-value:2.11E-5 ID:SGTC_2820|Compound:7989630|FD-Score:-3.92|P-value:7.84E-5 ID:SGTC_2821|Compound:7996764|FD-Score:4.61|P-value:3.83E-6 ID:SGTC_2834|Compound:9001755|FD-Score:4.09|P-value:3.60E-5 ID:SGTC_2870|Compound:9038528|FD-Score:4.89|P-value:1.06E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-4.76|P-value:2.15E-6 ID:SGTC_2917|Compound:7979103|FD-Score:7.21|P-value:1.39E-12 ID:SGTC_2921|Compound:7974240|FD-Score:4.62|P-value:3.68E-6 ID:SGTC_2923|Compound:7992654|FD-Score:5.80|P-value:9.07E-9 ID:SGTC_2926|Compound:9010726|FD-Score:5.06|P-value:4.52E-7 ID:SGTC_3018|Compound:9082602|FD-Score:-5.99|P-value:3.54E-9 ID:SGTC_3037|Compound:9091026|FD-Score:-5.02|P-value:6.28E-7 ID:SGTC_3167|Compound:9103034|FD-Score:3.23|P-value:8.46E-4 ID:SGTC_3186|Compound:9110464|FD-Score:-3.38|P-value:5.63E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.53|P-value:3.30E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.89|P-value:8.81E-5 ID:SGTC_3274|Compound:9138298|FD-Score:3.88|P-value:8.20E-5 ID:SGTC_3330|Compound:9141424|FD-Score:3.97|P-value:5.82E-5

Top fitness defect scores for YGL211W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 15.70 4.0 nM 1.22E-52 12 5.77E-56
2 SGTC_2679 rapamycin 9.84 1.0 nM 7.41E-22 28 3.77E-23
3 SGTC_2688 kanamycin b 7.79 96.7 µM 2.15E-14 calcium & mitochondrial duress 73 3.34E-15
4 SGTC_2917 7979103 7.21 53.8 µM 1.39E-12 2 2.83E-13
5 SGTC_2565 isoliquiritigenin 6.49 100.0 µM 1.51E-10 8 4.17E-11
6 SGTC_2923 7992654 5.80 58.1 µM 9.07E-9 Golgi 6 3.25E-9
7 SGTC_2243 6634756 5.29 200.0 µM 1.47E-7 5 6.28E-8
8 SGTC_2344 7990960 5.25 77.0 µM 1.81E-7 11 7.81E-8
9 SGTC_2926 9010726 5.06 65.2 µM 4.52E-7 15 2.07E-7
10 SGTC_2470 5664817 4.92 21.7 µM 8.99E-7 5 4.30E-7
11 SGTC_2870 9038528 4.89 13.0 µM 1.06E-6 9 5.14E-7
12 SGTC_800 3448-7362 4.83 155.0 µM 1.42E-6 translation 98 6.97E-7
13 SGTC_2921 7974240 4.62 10.7 µM 3.68E-6 33 1.92E-6
14 SGTC_2821 7996764 4.61 71.4 µM 3.83E-6 21 2.01E-6
15 SGTC_2029 5175081 4.48 200.0 µM 6.80E-6 7 3.69E-6
16 SGTC_2788 5282734 4.22 71.4 µM 2.11E-5 34 1.22E-5
17 SGTC_2247 cerulenin 4.21 830.0 nM 2.23E-5 13 1.30E-5
18 SGTC_2834 9001755 4.09 58.4 µM 3.60E-5 31 2.16E-5
19 SGTC_1564 D-Arginine 4.08 115.0 µM 3.69E-5 24 2.22E-5
20 SGTC_3330 9141424 3.97 71.4 µM 5.82E-5 amide catabolism 16 3.60E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.349 1.59E-96 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.320 1.68E-80 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.319 3.35E-80 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.311 5.56E-76 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.310 1.88E-75 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.307 2.10E-74 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.290 3.35E-66 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.281 8.21E-62 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.272 3.52E-58 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.269 1.69E-56 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.260 6.96E-53 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.258 4.69E-52 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.255 6.01E-51 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.240 4.67E-45 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.239 6.82E-45 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors