YGL218W_d

Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL218W / YGL218W

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:5.86|P-value:2.29E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.04|P-value:7.46E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:4.13|P-value:1.95E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.79|P-value:4.75E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:7.35|P-value:1.19E-10 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.12|P-value:2.04E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:5.35|P-value:2.08E-6 ID:SGTC_341|Compound:1486-1430|FD-Score:4.02|P-value:2.83E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:5.17|P-value:4.46E-6 ID:SGTC_684|Compound:1140-0066|FD-Score:5.63|P-value:6.49E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:4.36|P-value:9.18E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.91|P-value:3.96E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.23|P-value:1.15E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:4.26|P-value:1.26E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:4.55|P-value:4.60E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.71|P-value:7.39E-4 ID:SGTC_1519|Compound:esterone|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.84|P-value:4.05E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.74|P-value:6.71E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.06|P-value:2.01E-4 ID:SGTC_1742|Compound:st038235|FD-Score:4.44|P-value:6.86E-5 ID:SGTC_1750|Compound:st081859|FD-Score:8.58|P-value:7.19E-14 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.18|P-value:3.33E-6 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:5.15|P-value:4.79E-6 ID:SGTC_1993|Compound:st073995|FD-Score:4.37|P-value:8.77E-5 ID:SGTC_2040|Compound:5100729|FD-Score:3.63|P-value:9.13E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.70|P-value:7.61E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.03|P-value:2.72E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.42|P-value:7.42E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.82|P-value:5.23E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.62|P-value:9.58E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.05|P-value:9.55E-8 ID:SGTC_2804|Compound:7954240|FD-Score:4.83|P-value:1.66E-5 ID:SGTC_3044|Compound:9093193|FD-Score:5.03|P-value:7.67E-6 ID:SGTC_3212|Compound:9128307|FD-Score:3.86|P-value:4.69E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:5.86|P-value:2.29E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.04|P-value:7.46E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:4.13|P-value:1.95E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.79|P-value:4.75E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:7.35|P-value:1.19E-10 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.12|P-value:2.04E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:5.35|P-value:2.08E-6 ID:SGTC_341|Compound:1486-1430|FD-Score:4.02|P-value:2.83E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:5.17|P-value:4.46E-6 ID:SGTC_684|Compound:1140-0066|FD-Score:5.63|P-value:6.49E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:4.36|P-value:9.18E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.91|P-value:3.96E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.23|P-value:1.15E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:4.26|P-value:1.26E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:4.55|P-value:4.60E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.71|P-value:7.39E-4 ID:SGTC_1519|Compound:esterone|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.84|P-value:4.05E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.74|P-value:6.71E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.06|P-value:2.01E-4 ID:SGTC_1742|Compound:st038235|FD-Score:4.44|P-value:6.86E-5 ID:SGTC_1750|Compound:st081859|FD-Score:8.58|P-value:7.19E-14 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.18|P-value:3.33E-6 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:5.15|P-value:4.79E-6 ID:SGTC_1993|Compound:st073995|FD-Score:4.37|P-value:8.77E-5 ID:SGTC_2040|Compound:5100729|FD-Score:3.63|P-value:9.13E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.70|P-value:7.61E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.03|P-value:2.72E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.42|P-value:7.42E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.82|P-value:5.23E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.62|P-value:9.58E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.05|P-value:9.55E-8 ID:SGTC_2804|Compound:7954240|FD-Score:4.83|P-value:1.66E-5 ID:SGTC_3044|Compound:9093193|FD-Score:5.03|P-value:7.67E-6 ID:SGTC_3212|Compound:9128307|FD-Score:3.86|P-value:4.69E-4

Top fitness defect scores for YGL218W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 8.58 2.4 µM 7.19E-14 endomembrane recycling 57 4.84E-18
2 SGTC_236 1509-0011 7.35 5.3 µM 1.19E-10 261 9.62E-14
3 SGTC_2688 kanamycin b 6.05 96.7 µM 9.55E-8 calcium & mitochondrial duress 158 7.17E-10
4 SGTC_10 0279-0212 5.86 42.6 µM 2.29E-7 TRP & mitochondrial translation 31 2.29E-9
5 SGTC_684 1140-0066 5.63 328.0 µM 6.49E-7 Golgi 4 9.20E-9
6 SGTC_314 1671-0008 5.35 2.7 µM 2.08E-6 13 4.35E-8
7 SGTC_680 0417-1641 5.17 114.0 µM 4.46E-6 RPP1 & pyrimidine depletion 3 1.20E-7
8 SGTC_1883 5-benzylidenerhodanine 5.15 20.0 µM 4.79E-6 TRP & mitochondrial translation 27 1.31E-7
9 SGTC_28 pentachloropyridine N-oxide 5.04 1.6 µM 7.46E-6 TSC3-RPN4 28 2.37E-7
10 SGTC_3044 9093193 5.03 49.5 µM 7.67E-6 9 2.46E-7
11 SGTC_2804 7954240 4.83 19.5 µM 1.66E-5 14 6.89E-7
12 SGTC_1164 k060-0068 4.55 93.7 µM 4.60E-5 13 2.66E-6
13 SGTC_1742 st038235 4.44 28.9 µM 6.86E-5 mitochondrial stress 98 4.52E-6
14 SGTC_2540 epiandrosterone 4.42 100.0 µM 7.42E-5 44 5.02E-6
15 SGTC_1993 st073995 4.37 70.8 µM 8.77E-5 15 6.26E-6
16 SGTC_750 4073-0104 4.36 132.0 µM 9.18E-5 8 6.65E-6
17 SGTC_1075 aripiprazole 4.26 15.2 µM 1.26E-4 20 1.02E-5
18 SGTC_1519 esterone 4.21 74.0 µM 1.50E-4 DNA intercalators 14 1.27E-5
19 SGTC_68 0986-0284 4.13 82.8 µM 1.95E-4 63 1.80E-5
20 SGTC_275 trichlorophene 4.12 5.7 µM 2.04E-4 mitochondrial stress 220 1.91E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.325 2.30E-83 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.319 7.33E-80 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.316 1.63E-78 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.315 4.21E-78 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.301 4.26E-71 YGR101W PCP1 hom Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
0.298 9.34E-70 YMR064W AEP1 hom Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase
0.297 2.43E-69 YDR268W MSW1 hom Mitochondrial tryptophanyl-tRNA synthetase
0.295 2.03E-68 YDR298C ATP5 hom Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.293 1.70E-67 YER028C MIG3 hom Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
0.291 2.89E-66 YPL183W-A RTC6 hom Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
0.290 6.48E-66 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.288 3.74E-65 YLR091W GEP5 hom Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.288 5.67E-65 YLL033W IRC19 hom Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
0.288 7.50E-65 YLR149C YLR149C hom Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
0.283 1.21E-62 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly