YGL221C / NIF3

Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL221C / NIF3

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.13|P-value:5.07E-4 ID:SGTC_12|Compound:0849-0099|FD-Score:3.75|P-value:4.03E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:4.44|P-value:1.49E-6 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.13|P-value:4.69E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:6.57|P-value:2.37E-12 ID:SGTC_536|Compound:3852-0578|FD-Score:3.66|P-value:6.06E-5 ID:SGTC_607|Compound:4491-0986|FD-Score:6.24|P-value:2.54E-11 ID:SGTC_674|Compound:0498-0004|FD-Score:3.64|P-value:6.64E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.96|P-value:1.57E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.88|P-value:2.03E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.04|P-value:6.54E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:4.30|P-value:3.16E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:5.41|P-value:6.40E-9 ID:SGTC_919|Compound:tnp00227|FD-Score:7.18|P-value:2.08E-14 ID:SGTC_948|Compound:3448-0295|FD-Score:4.36|P-value:2.31E-6 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.29|P-value:2.78E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:-3.64|P-value:5.75E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.03|P-value:7.27E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.33|P-value:2.09E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.63|P-value:6.79E-5 ID:SGTC_1296|Compound:1068-0102|FD-Score:5.94|P-value:2.04E-10 ID:SGTC_1298|Compound:1079-1278|FD-Score:-4.39|P-value:1.71E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.09|P-value:5.90E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.83|P-value:1.86E-7 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.24|P-value:3.28E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.29|P-value:2.73E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.21|P-value:4.85E-6 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.28|P-value:2.84E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:-4.42|P-value:1.49E-6 ID:SGTC_1628|Compound:st004252|FD-Score:3.19|P-value:4.06E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-3.30|P-value:2.38E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.30|P-value:2.63E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.60|P-value:7.63E-5 ID:SGTC_1774|Compound:st048371|FD-Score:4.72|P-value:3.38E-7 ID:SGTC_1836|Compound:st055642|FD-Score:-3.28|P-value:2.63E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.70|P-value:5.12E-5 ID:SGTC_2057|Compound:5236571|FD-Score:3.00|P-value:8.10E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.59|P-value:8.11E-5 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-6.40|P-value:6.93E-12 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.57|P-value:7.59E-7 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.05|P-value:1.02E-5 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.35|P-value:1.93E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.00|P-value:7.32E-4 ID:SGTC_2815|Compound:7968870|FD-Score:2.97|P-value:9.04E-4 ID:SGTC_2853|Compound:9015042|FD-Score:3.12|P-value:5.19E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.01|P-value:7.79E-4 ID:SGTC_3073|Compound:9114972|FD-Score:2.98|P-value:8.84E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.19|P-value:4.12E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.01|P-value:7.91E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.42|P-value:1.49E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.13|P-value:5.07E-4 ID:SGTC_12|Compound:0849-0099|FD-Score:3.75|P-value:4.03E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:4.44|P-value:1.49E-6 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.13|P-value:4.69E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:6.57|P-value:2.37E-12 ID:SGTC_536|Compound:3852-0578|FD-Score:3.66|P-value:6.06E-5 ID:SGTC_607|Compound:4491-0986|FD-Score:6.24|P-value:2.54E-11 ID:SGTC_674|Compound:0498-0004|FD-Score:3.64|P-value:6.64E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.96|P-value:1.57E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.88|P-value:2.03E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.04|P-value:6.54E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:4.30|P-value:3.16E-6 ID:SGTC_911|Compound:3456-4043|FD-Score:5.41|P-value:6.40E-9 ID:SGTC_919|Compound:tnp00227|FD-Score:7.18|P-value:2.08E-14 ID:SGTC_948|Compound:3448-0295|FD-Score:4.36|P-value:2.31E-6 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.29|P-value:2.78E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:-3.64|P-value:5.75E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.03|P-value:7.27E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.33|P-value:2.09E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.63|P-value:6.79E-5 ID:SGTC_1296|Compound:1068-0102|FD-Score:5.94|P-value:2.04E-10 ID:SGTC_1298|Compound:1079-1278|FD-Score:-4.39|P-value:1.71E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.09|P-value:5.90E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.83|P-value:1.86E-7 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.24|P-value:3.28E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.29|P-value:2.73E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.21|P-value:4.85E-6 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.28|P-value:2.84E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:-4.42|P-value:1.49E-6 ID:SGTC_1628|Compound:st004252|FD-Score:3.19|P-value:4.06E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-3.30|P-value:2.38E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.30|P-value:2.63E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.60|P-value:7.63E-5 ID:SGTC_1774|Compound:st048371|FD-Score:4.72|P-value:3.38E-7 ID:SGTC_1836|Compound:st055642|FD-Score:-3.28|P-value:2.63E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.70|P-value:5.12E-5 ID:SGTC_2057|Compound:5236571|FD-Score:3.00|P-value:8.10E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.59|P-value:8.11E-5 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-6.40|P-value:6.93E-12 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.57|P-value:7.59E-7 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.05|P-value:1.02E-5 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.35|P-value:1.93E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.00|P-value:7.32E-4 ID:SGTC_2815|Compound:7968870|FD-Score:2.97|P-value:9.04E-4 ID:SGTC_2853|Compound:9015042|FD-Score:3.12|P-value:5.19E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.01|P-value:7.79E-4 ID:SGTC_3073|Compound:9114972|FD-Score:2.98|P-value:8.84E-4 ID:SGTC_3251|Compound:9135047|FD-Score:3.19|P-value:4.12E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.01|P-value:7.91E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.42|P-value:1.49E-4

Top fitness defect scores for YGL221C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_919 tnp00227 7.18 403.6 nM 2.08E-14 RNA pol III & RNase P/MRP 3 3.51E-13
2 SGTC_430 0958-0115 6.57 16.4 µM 2.37E-12 5 2.53E-11
3 SGTC_607 4491-0986 6.24 66.8 µM 2.54E-11 heme requiring 6 2.15E-10
4 SGTC_1296 1068-0102 5.94 11.9 µM 2.04E-10 1 1.41E-9
5 SGTC_911 3456-4043 5.41 236.0 µM 6.40E-9 mitochondrial processes 7 3.18E-8
6 SGTC_1390 3413-2614 4.83 145.0 µM 1.86E-7 fatty acid desaturase (OLE1) 11 6.70E-7
7 SGTC_1774 st048371 4.72 48.5 µM 3.38E-7 RNA processing & uracil transport 6 1.15E-6
8 SGTC_2536 dehydroabietamide 4.57 32.0 µM 7.59E-7 16 2.40E-6
9 SGTC_245 methotrexate 4.44 289.6 µM 1.49E-6 32 4.40E-6
10 SGTC_948 3448-0295 4.36 75.3 µM 2.31E-6 17 6.57E-6
11 SGTC_909 3448-9333 4.30 149.0 µM 3.16E-6 13 8.71E-6
12 SGTC_1479 0549-0158 4.21 134.0 µM 4.85E-6 18 1.29E-5
13 SGTC_2629 bisabolol 4.05 100.0 µM 1.02E-5 18 2.52E-5
14 SGTC_814 3342-0569 3.96 96.0 µM 1.57E-5 19 3.72E-5
15 SGTC_12 0849-0099 3.75 9.3 µM 4.03E-5 22 8.77E-5
16 SGTC_1916 st067606 3.70 49.2 µM 5.12E-5 50 1.09E-4
17 SGTC_536 3852-0578 3.66 10.6 µM 6.06E-5 37 1.27E-4
18 SGTC_674 0498-0004 3.64 451.0 µM 6.64E-5 heme requiring 29 1.38E-4
19 SGTC_1225 1216-0074 3.63 20.6 µM 6.79E-5 32 1.41E-4
20 SGTC_1681 tripelennamine 3.60 100.0 µM 7.63E-5 29 1.57E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.100 6.92E-9 YLR018C POM34 hom Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
0.094 4.70E-8 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.088 2.83E-7 YLL019C KNS1 hom Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
0.086 5.95E-7 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.082 2.00E-6 YPR155C NCA2 hom Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
0.079 4.33E-6 YBR174C_d YBR174C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
0.079 4.79E-6 YOR078W BUD21 hom Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.078 5.69E-6 YCR045C RRT12 hom Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores
0.077 8.45E-6 YDL046W NPC2 hom Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes
0.077 8.85E-6 YGL051W MST27 hom Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles
0.075 1.49E-5 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.075 1.52E-5 YER002W NOP16 hom Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
0.073 2.14E-5 YOL028C YAP7 hom Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
0.070 4.80E-5 YHL028W WSC4 hom ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
0.067 1.05E-4 YDR399W HPT1 hom Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome