YGL227W / VID30

Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL227W / VID30

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:4.09|P-value:2.13E-5 ID:SGTC_22|Compound:0964-0034|FD-Score:3.65|P-value:1.30E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.28|P-value:5.07E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.03|P-value:2.24E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:4.56|P-value:2.56E-6 ID:SGTC_129|Compound:0088-0017|FD-Score:5.05|P-value:2.10E-7 ID:SGTC_203|Compound:k066-0004|FD-Score:4.61|P-value:2.00E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:4.40|P-value:5.42E-6 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.37|P-value:3.60E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.90|P-value:4.84E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:3.67|P-value:1.20E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:3.38|P-value:3.58E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.80|P-value:7.22E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:3.40|P-value:3.34E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.55|P-value:1.92E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.59|P-value:2.18E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:-3.23|P-value:5.86E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.63|P-value:1.40E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:5.51|P-value:1.69E-8 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.69|P-value:1.13E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.48|P-value:2.54E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.58|P-value:1.66E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.72|P-value:1.00E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:-3.23|P-value:5.86E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-4.23|P-value:1.09E-5 ID:SGTC_1500|Compound:4488-1866|FD-Score:-3.09|P-value:9.54E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.81|P-value:6.82E-5 ID:SGTC_1750|Compound:st081859|FD-Score:5.52|P-value:1.63E-8 ID:SGTC_1755|Compound:st041960|FD-Score:3.78|P-value:7.88E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.26|P-value:5.61E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.14|P-value:8.43E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.50|P-value:2.28E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.29|P-value:4.97E-4 ID:SGTC_2136|Compound:5328288|FD-Score:3.88|P-value:5.23E-5 ID:SGTC_2156|Compound:5649887|FD-Score:4.54|P-value:2.71E-6 ID:SGTC_2166|Compound:5933061|FD-Score:3.18|P-value:7.29E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.37|P-value:3.71E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-3.19|P-value:6.79E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.44|P-value:2.81E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.98|P-value:3.39E-5 ID:SGTC_2961|Compound:9083290|FD-Score:-4.21|P-value:1.20E-5 ID:SGTC_2965|Compound:9082772|FD-Score:5.22|P-value:8.52E-8 ID:SGTC_3017|Compound:9080681|FD-Score:3.77|P-value:8.17E-5 ID:SGTC_3202|Compound:9113761|FD-Score:3.20|P-value:6.92E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.92|P-value:4.26E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-5.38|P-value:3.50E-8 ID:SGTC_8|Compound:0354-0001|FD-Score:4.09|P-value:2.13E-5 ID:SGTC_22|Compound:0964-0034|FD-Score:3.65|P-value:1.30E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.28|P-value:5.07E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.03|P-value:2.24E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:4.56|P-value:2.56E-6 ID:SGTC_129|Compound:0088-0017|FD-Score:5.05|P-value:2.10E-7 ID:SGTC_203|Compound:k066-0004|FD-Score:4.61|P-value:2.00E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:4.40|P-value:5.42E-6 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.37|P-value:3.60E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.90|P-value:4.84E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:3.67|P-value:1.20E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:3.38|P-value:3.58E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.80|P-value:7.22E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:3.40|P-value:3.34E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.55|P-value:1.92E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.59|P-value:2.18E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:-3.23|P-value:5.86E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.63|P-value:1.40E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:5.51|P-value:1.69E-8 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.69|P-value:1.13E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.48|P-value:2.54E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.58|P-value:1.66E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.72|P-value:1.00E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:-3.23|P-value:5.86E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-4.23|P-value:1.09E-5 ID:SGTC_1500|Compound:4488-1866|FD-Score:-3.09|P-value:9.54E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.81|P-value:6.82E-5 ID:SGTC_1750|Compound:st081859|FD-Score:5.52|P-value:1.63E-8 ID:SGTC_1755|Compound:st041960|FD-Score:3.78|P-value:7.88E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.26|P-value:5.61E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.14|P-value:8.43E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.50|P-value:2.28E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.29|P-value:4.97E-4 ID:SGTC_2136|Compound:5328288|FD-Score:3.88|P-value:5.23E-5 ID:SGTC_2156|Compound:5649887|FD-Score:4.54|P-value:2.71E-6 ID:SGTC_2166|Compound:5933061|FD-Score:3.18|P-value:7.29E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.37|P-value:3.71E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-3.19|P-value:6.79E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-3.44|P-value:2.81E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.98|P-value:3.39E-5 ID:SGTC_2961|Compound:9083290|FD-Score:-4.21|P-value:1.20E-5 ID:SGTC_2965|Compound:9082772|FD-Score:5.22|P-value:8.52E-8 ID:SGTC_3017|Compound:9080681|FD-Score:3.77|P-value:8.17E-5 ID:SGTC_3202|Compound:9113761|FD-Score:3.20|P-value:6.92E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.92|P-value:4.26E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-5.38|P-value:3.50E-8

Top fitness defect scores for YGL227W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 5.52 2.4 µM 1.63E-8 endomembrane recycling 120 1.69E-8
2 SGTC_1064 honokiol 5.51 6.8 µM 1.69E-8 6 1.74E-8
3 SGTC_2965 9082772 5.22 38.6 µM 8.52E-8 mitochondrial response to ROS 15 8.77E-8
4 SGTC_129 0088-0017 5.05 176.6 µM 2.10E-7 44 2.16E-7
5 SGTC_203 k066-0004 4.61 30.9 µM 2.00E-6 RSC & ERG11 9 2.04E-6
6 SGTC_682 0kpi-0099 4.59 17.0 µM 2.18E-6 51 2.22E-6
7 SGTC_87 0100-0052 4.56 35.5 µM 2.56E-6 69 2.61E-6
8 SGTC_2156 5649887 4.54 115.0 µM 2.71E-6 RPP1 & pyrimidine depletion 4 2.76E-6
9 SGTC_207 4185-0010 4.40 4.5 µM 5.42E-6 endomembrane recycling 73 5.51E-6
10 SGTC_8 0354-0001 4.09 70.0 µM 2.13E-5 73 2.15E-5
11 SGTC_2932 9016711 3.98 44.5 µM 3.39E-5 46 3.43E-5
12 SGTC_329 k048-0121 3.90 21.3 µM 4.84E-5 RSC & ERG11 13 4.90E-5
13 SGTC_2136 5328288 3.88 199.3 µM 5.23E-5 23 5.29E-5
14 SGTC_1519 esterone 3.81 74.0 µM 6.82E-5 DNA intercalators 18 6.89E-5
15 SGTC_564 3555-0129 3.80 330.0 µM 7.22E-5 5 7.30E-5
16 SGTC_1755 st041960 3.78 45.2 µM 7.88E-5 30 7.96E-5
17 SGTC_3017 9080681 3.77 49.5 µM 8.17E-5 17 8.25E-5
18 SGTC_1261 0712-0010 3.72 12.7 µM 1.00E-4 RNA processing & uracil transport 28 1.01E-4
19 SGTC_1137 ixabepilone 3.69 100.0 µM 1.13E-4 37 1.14E-4
20 SGTC_344 k064-0035 3.67 6.4 µM 1.20E-4 60S ribosome export 28 1.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.224 1.48E-39 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.220 4.53E-38 YPR153W YPR153W hom Putative protein of unknown function
0.195 3.86E-30 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.165 8.87E-22 YBR255W MTC4 hom Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
0.159 1.88E-20 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.156 8.19E-20 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.149 4.45E-18 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.147 1.08E-17 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.143 9.87E-17 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.141 2.99E-16 YPR060C ARO7 hom Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
0.134 7.26E-15 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.133 1.05E-14 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.121 2.23E-12 YAL028W FRT2 hom Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication
0.118 6.67E-12 YNL056W OCA2 hom Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
0.118 8.04E-12 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C