YGL228W / SHE10

Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL228W / SHE10

Click on Significant Values for Screen Details ID:SGTC_255|Compound:tunicamycin|FD-Score:3.23|P-value:5.22E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.18|P-value:6.32E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.07|P-value:9.30E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.31|P-value:3.98E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.30|P-value:4.10E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:3.62|P-value:1.19E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:3.39|P-value:2.89E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.23|P-value:5.23E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-4.79|P-value:6.20E-7 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.42|P-value:2.62E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.22|P-value:5.47E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.56|P-value:1.84E-6 ID:SGTC_1472|Compound:k841-0093|FD-Score:4.48|P-value:2.75E-6 ID:SGTC_1642|Compound:st009582|FD-Score:-3.77|P-value:6.80E-5 ID:SGTC_1670|Compound:st016595|FD-Score:3.27|P-value:4.58E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.34|P-value:3.54E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.13|P-value:7.63E-4 ID:SGTC_1805|Compound:st050013|FD-Score:-3.07|P-value:9.36E-4 ID:SGTC_1975|Compound:st070967|FD-Score:4.60|P-value:1.53E-6 ID:SGTC_1985|Compound:st071382|FD-Score:3.84|P-value:4.99E-5 ID:SGTC_2065|Compound:5221596|FD-Score:-3.19|P-value:6.06E-4 ID:SGTC_2070|Compound:5230947|FD-Score:-3.19|P-value:6.15E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-4.65|P-value:1.25E-6 ID:SGTC_2366|Compound:9070441|FD-Score:3.12|P-value:7.63E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.90|P-value:1.12E-9 ID:SGTC_2429|Compound:5902844|FD-Score:5.17|P-value:7.83E-8 ID:SGTC_2436|Compound:5633522|FD-Score:5.79|P-value:2.23E-9 ID:SGTC_2468|Compound:5987765|FD-Score:4.12|P-value:1.50E-5 ID:SGTC_2483|Compound:5729050|FD-Score:-3.39|P-value:2.99E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:-5.94|P-value:8.92E-10 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.17|P-value:6.42E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:6.34|P-value:6.48E-11 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.36|P-value:3.32E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.12|P-value:8.02E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-4.75|P-value:7.64E-7 ID:SGTC_2969|Compound:9091408|FD-Score:3.08|P-value:8.95E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.27|P-value:4.57E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.37|P-value:3.21E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.23|P-value:5.22E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.18|P-value:6.32E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.07|P-value:9.30E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.31|P-value:3.98E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.30|P-value:4.10E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:3.63|P-value:1.16E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:3.62|P-value:1.19E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:3.39|P-value:2.89E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.23|P-value:5.23E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-4.79|P-value:6.20E-7 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.42|P-value:2.62E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:-3.22|P-value:5.47E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.56|P-value:1.84E-6 ID:SGTC_1472|Compound:k841-0093|FD-Score:4.48|P-value:2.75E-6 ID:SGTC_1642|Compound:st009582|FD-Score:-3.77|P-value:6.80E-5 ID:SGTC_1670|Compound:st016595|FD-Score:3.27|P-value:4.58E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.34|P-value:3.54E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.13|P-value:7.63E-4 ID:SGTC_1805|Compound:st050013|FD-Score:-3.07|P-value:9.36E-4 ID:SGTC_1975|Compound:st070967|FD-Score:4.60|P-value:1.53E-6 ID:SGTC_1985|Compound:st071382|FD-Score:3.84|P-value:4.99E-5 ID:SGTC_2065|Compound:5221596|FD-Score:-3.19|P-value:6.06E-4 ID:SGTC_2070|Compound:5230947|FD-Score:-3.19|P-value:6.15E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-4.65|P-value:1.25E-6 ID:SGTC_2366|Compound:9070441|FD-Score:3.12|P-value:7.63E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.90|P-value:1.12E-9 ID:SGTC_2429|Compound:5902844|FD-Score:5.17|P-value:7.83E-8 ID:SGTC_2436|Compound:5633522|FD-Score:5.79|P-value:2.23E-9 ID:SGTC_2468|Compound:5987765|FD-Score:4.12|P-value:1.50E-5 ID:SGTC_2483|Compound:5729050|FD-Score:-3.39|P-value:2.99E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:-5.94|P-value:8.92E-10 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.17|P-value:6.42E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:6.34|P-value:6.48E-11 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.36|P-value:3.32E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.12|P-value:8.02E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-4.75|P-value:7.64E-7 ID:SGTC_2969|Compound:9091408|FD-Score:3.08|P-value:8.95E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.27|P-value:4.57E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.37|P-value:3.21E-4

Top fitness defect scores for YGL228W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2502 avocadyne acetate 6.34 18.6 µM 6.48E-11 6 1.15E-10
2 SGTC_2422 chlorphenesin carbamate 5.90 384.2 µM 1.12E-9 6 1.86E-9
3 SGTC_2436 5633522 5.79 8.1 µM 2.23E-9 5 3.62E-9
4 SGTC_2429 5902844 5.17 64.4 µM 7.83E-8 8 1.16E-7
5 SGTC_1975 st070967 4.60 30.8 µM 1.53E-6 21 2.09E-6
6 SGTC_1264 0783-0040 4.56 158.0 µM 1.84E-6 12 2.51E-6
7 SGTC_1472 k841-0093 4.48 193.0 µM 2.75E-6 9 3.70E-6
8 SGTC_2468 5987765 4.12 72.9 µM 1.50E-5 10 1.93E-5
9 SGTC_1985 st071382 3.84 65.5 µM 4.99E-5 22 6.23E-5
10 SGTC_672 1441-0018 3.63 53.7 µM 1.16E-4 23 1.42E-4
11 SGTC_919 tnp00227 3.62 403.6 nM 1.19E-4 RNA pol III & RNase P/MRP 50 1.46E-4
12 SGTC_1012 4112-3522 3.39 18.0 µM 2.89E-4 41 3.45E-4
13 SGTC_3113 9123498 3.37 49.5 µM 3.21E-4 30 3.82E-4
14 SGTC_2539 epi(13)torulosol 3.36 80.0 µM 3.32E-4 44 3.95E-4
15 SGTC_1732 st034308 3.34 51.8 µM 3.54E-4 49 4.20E-4
16 SGTC_441 propafenone 3.31 146.0 µM 3.98E-4 9 4.71E-4
17 SGTC_612 0302-0167 3.30 22.4 µM 4.10E-4 copper-dependent oxidative stress 64 4.85E-4
18 SGTC_3101 9120531 3.27 49.5 µM 4.57E-4 56 5.39E-4
19 SGTC_1670 st016595 3.27 4.0 µM 4.58E-4 27 5.41E-4
20 SGTC_255 tunicamycin 3.23 200.5 nM 5.22E-4 unfolded protein response 112 6.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.117 1.01E-11 YJR068W RFC2 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.098 1.32E-8 YCR095W-A_p YCR095W-A_p hom Putative protein of unknown function
0.092 8.16E-8 YOR378W_p YOR378W_p hom Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene
0.090 2.02E-7 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.082 1.77E-6 YER096W SHC1 hom Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
0.081 2.30E-6 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.077 7.45E-6 YNR057C BIO4 hom Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels
0.074 1.59E-5 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.073 2.53E-5 YGL208W SIP2 hom One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane
0.072 2.69E-5 YHL017W_p YHL017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
0.071 3.41E-5 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.071 3.82E-5 YHR178W STB5 hom Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro
0.071 3.79E-5 YLR210W CLB4 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation
0.069 5.79E-5 YNL085W MKT1 hom Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress
0.068 7.59E-5 YPL076W GPI2 het Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein