YGL233W / SEC15

Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL233W / SEC15

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:7.82|P-value:1.15E-19 ID:SGTC_207|Compound:4185-0010|FD-Score:3.40|P-value:4.54E-5 ID:SGTC_252|Compound:miconazole|FD-Score:5.51|P-value:1.17E-10 ID:SGTC_256|Compound:clotrimazole|FD-Score:2.77|P-value:7.19E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.19|P-value:1.15E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:5.15|P-value:1.49E-9 ID:SGTC_367|Compound:0335-0881|FD-Score:3.17|P-value:1.29E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.52|P-value:2.73E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:3.34|P-value:5.99E-5 ID:SGTC_493|Compound:zm226600|FD-Score:3.59|P-value:1.79E-5 ID:SGTC_538|Compound:2922-0838|FD-Score:3.03|P-value:2.36E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.01|P-value:2.63E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:-2.79|P-value:6.86E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-2.94|P-value:3.80E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.10|P-value:1.76E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.09|P-value:1.82E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:2.85|P-value:5.13E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:8.60|P-value:2.31E-23 ID:SGTC_1204|Compound:4049-0315|FD-Score:-4.13|P-value:1.11E-6 ID:SGTC_1213|Compound:1315-0166|FD-Score:2.96|P-value:3.26E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.55|P-value:2.18E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.04|P-value:2.29E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-6.07|P-value:1.63E-12 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.68|P-value:1.21E-5 ID:SGTC_1379|Compound:2144-0755|FD-Score:2.74|P-value:8.05E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.32|P-value:6.41E-5 ID:SGTC_1531|Compound:st059297|FD-Score:2.76|P-value:7.37E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.08|P-value:1.91E-4 ID:SGTC_1657|Compound:st012939|FD-Score:3.22|P-value:1.04E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:2.77|P-value:6.94E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-2.84|P-value:5.63E-4 ID:SGTC_1960|Compound:st011366|FD-Score:-2.78|P-value:7.18E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-3.43|P-value:4.22E-5 ID:SGTC_2008|Compound:4012253|FD-Score:4.10|P-value:1.21E-6 ID:SGTC_2010|Compound:4012422|FD-Score:-3.43|P-value:4.24E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-2.81|P-value:6.47E-4 ID:SGTC_2036|Compound:5160441|FD-Score:2.82|P-value:5.79E-4 ID:SGTC_2127|Compound:5253358|FD-Score:-2.71|P-value:9.55E-4 ID:SGTC_2180|Compound:6301957|FD-Score:2.79|P-value:6.64E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.60|P-value:5.76E-11 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.82|P-value:5.33E-6 ID:SGTC_2273|Compound:7815444|FD-Score:2.79|P-value:6.45E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.17|P-value:1.38E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.65|P-value:1.33E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-2.71|P-value:9.51E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.51|P-value:2.59E-5 ID:SGTC_2438|Compound:5259590|FD-Score:2.76|P-value:7.27E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.25|P-value:8.89E-5 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:2.87|P-value:4.76E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:5.37|P-value:3.16E-10 ID:SGTC_2839|Compound:9003951|FD-Score:3.49|P-value:2.88E-5 ID:SGTC_3014|Compound:9081533|FD-Score:2.71|P-value:9.06E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.25|P-value:9.14E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.09|P-value:1.88E-4 ID:SGTC_3332|Compound:9141069|FD-Score:2.79|P-value:6.60E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.31|P-value:6.99E-5 ID:SGTC_57|Compound:miconazole|FD-Score:7.82|P-value:1.15E-19 ID:SGTC_207|Compound:4185-0010|FD-Score:3.40|P-value:4.54E-5 ID:SGTC_252|Compound:miconazole|FD-Score:5.51|P-value:1.17E-10 ID:SGTC_256|Compound:clotrimazole|FD-Score:2.77|P-value:7.19E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.19|P-value:1.15E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:5.15|P-value:1.49E-9 ID:SGTC_367|Compound:0335-0881|FD-Score:3.17|P-value:1.29E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.52|P-value:2.73E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:3.34|P-value:5.99E-5 ID:SGTC_493|Compound:zm226600|FD-Score:3.59|P-value:1.79E-5 ID:SGTC_538|Compound:2922-0838|FD-Score:3.03|P-value:2.36E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.01|P-value:2.63E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:-2.79|P-value:6.86E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-2.94|P-value:3.80E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.10|P-value:1.76E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.09|P-value:1.82E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:2.85|P-value:5.13E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:8.60|P-value:2.31E-23 ID:SGTC_1204|Compound:4049-0315|FD-Score:-4.13|P-value:1.11E-6 ID:SGTC_1213|Compound:1315-0166|FD-Score:2.96|P-value:3.26E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.55|P-value:2.18E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.04|P-value:2.29E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-6.07|P-value:1.63E-12 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.68|P-value:1.21E-5 ID:SGTC_1379|Compound:2144-0755|FD-Score:2.74|P-value:8.05E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.32|P-value:6.41E-5 ID:SGTC_1531|Compound:st059297|FD-Score:2.76|P-value:7.37E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.08|P-value:1.91E-4 ID:SGTC_1657|Compound:st012939|FD-Score:3.22|P-value:1.04E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:2.77|P-value:6.94E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-2.84|P-value:5.63E-4 ID:SGTC_1960|Compound:st011366|FD-Score:-2.78|P-value:7.18E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-3.43|P-value:4.22E-5 ID:SGTC_2008|Compound:4012253|FD-Score:4.10|P-value:1.21E-6 ID:SGTC_2010|Compound:4012422|FD-Score:-3.43|P-value:4.24E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-2.81|P-value:6.47E-4 ID:SGTC_2036|Compound:5160441|FD-Score:2.82|P-value:5.79E-4 ID:SGTC_2127|Compound:5253358|FD-Score:-2.71|P-value:9.55E-4 ID:SGTC_2180|Compound:6301957|FD-Score:2.79|P-value:6.64E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.60|P-value:5.76E-11 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.82|P-value:5.33E-6 ID:SGTC_2273|Compound:7815444|FD-Score:2.79|P-value:6.45E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.17|P-value:1.38E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.65|P-value:1.33E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-2.71|P-value:9.51E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.51|P-value:2.59E-5 ID:SGTC_2438|Compound:5259590|FD-Score:2.76|P-value:7.27E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.25|P-value:8.89E-5 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:2.87|P-value:4.76E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:5.37|P-value:3.16E-10 ID:SGTC_2839|Compound:9003951|FD-Score:3.49|P-value:2.88E-5 ID:SGTC_3014|Compound:9081533|FD-Score:2.71|P-value:9.06E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.25|P-value:9.14E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.09|P-value:1.88E-4 ID:SGTC_3332|Compound:9141069|FD-Score:2.79|P-value:6.60E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.31|P-value:6.99E-5

Top fitness defect scores for YGL233W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1072 oxiconazole 8.60 14.9 nM 2.31E-23 ergosterol depletion effects on membrane 5 4.02E-18 0.59
2 SGTC_57 miconazole 7.82 162.5 nM 1.15E-19 ergosterol depletion effects on membrane 10 2.57E-15 0.13
3 SGTC_2249 haloperidol 5.60 116.4 µM 5.76E-11 ergosterol depletion effects on membrane 20 1.06E-8 0.10
4 SGTC_252 miconazole 5.51 1.3 nM 1.17E-10 ergosterol depletion effects on membrane 9 1.82E-8 0.06
5 SGTC_2778 tioconazole 5.37 65.1 nM 3.16E-10 ergosterol depletion effects on membrane 15 3.88E-8 0.16
6 SGTC_312 4092-0653 5.19 6.8 µM 1.15E-9 ergosterol depletion effects on membrane 18 1.04E-7 0.02
7 SGTC_320 4092-0846 5.15 5.3 µM 1.49E-9 ergosterol depletion effects on membrane 14 1.27E-7 0.58
8 SGTC_2008 4012253 4.10 172.0 µM 1.21E-6 12 2.11E-5 0.04
9 SGTC_2250 alverine citrate 3.82 130.0 µM 5.33E-6 36 6.56E-5 0.15
10 SGTC_2413 inermin 3.65 26.1 µM 1.33E-5 22 1.32E-4 0.08
11 SGTC_493 zm226600 3.59 134.0 µM 1.79E-5 mitochondrial processes 8 1.66E-4 0.16
12 SGTC_1245 0418-0076 3.55 17.2 µM 2.18E-5 ERG2 12 1.93E-4 0.00
13 SGTC_2436 5633522 3.51 8.1 µM 2.59E-5 16 2.21E-4 0.01
14 SGTC_2839 9003951 3.49 26.0 µM 2.88E-5 6 2.40E-4 0.08
15 SGTC_207 4185-0010 3.40 4.5 µM 4.54E-5 endomembrane recycling 24 3.39E-4 0.10
16 SGTC_454 lyso-paf C-16 3.34 6.7 µM 5.99E-5 9 4.20E-4 0.07
17 SGTC_1396 3511-0007 3.32 98.6 µM 6.41E-5 mitochondrial processes 7 4.42E-4 0.09
18 SGTC_3344 9148862 3.31 3.9 µM 6.99E-5 7 4.73E-4 0.08
19 SGTC_2523 spermine 3.25 47.7 µM 8.89E-5 calcium & mitochondrial duress 4 5.69E-4 0.13
20 SGTC_3152 9099253 3.25 49.5 µM 9.14E-5 NEO1-PIK1 38 5.81E-4 0.18

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.428 6.86E-150 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.427 1.66E-148 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.405 1.04E-132 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.357 1.28E-101 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.355 3.34E-100 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.352 2.61E-98 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.348 4.78E-96 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.331 7.74E-87 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.300 9.81E-71 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.299 2.19E-70 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.283 5.69E-63 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.269 9.92E-57 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.265 7.78E-55 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.251 4.11E-49 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.243 2.32E-46 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress