HIPHOP chemogenomics database

YGL236C / MTO1

Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL236C / MTO1

Click on Significant Values for Screen Details

Top fitness defect scores for YGL236C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2587	piperic acid	6.50	92.2 µM	4.63E-10		2	3.99E-11
2	SGTC_832	4083-0019	5.08	164.0 µM	8.94E-7	translation	36	1.93E-7
3	SGTC_625	k213-0058	4.93	121.0 µM	1.79E-6	translation	58	4.20E-7
4	SGTC_1092	toremifene	4.73	6.3 µM	4.30E-6		9	1.12E-6
5	SGTC_2385	9074692	4.59	200.0 µM	7.85E-6		29	2.20E-6
6	SGTC_1107	amlodipine	4.53	47.0 µM	1.04E-5		21	3.02E-6
7	SGTC_800	3448-7362	4.51	155.0 µM	1.10E-5	translation	116	3.23E-6
8	SGTC_2942	9052777	4.36	62.2 µM	2.06E-5		24	6.49E-6
9	SGTC_204	1543-0184	4.27	62.4 µM	3.00E-5	TRP & mitochondrial translation	25	9.90E-6
10	SGTC_3159	9100096	4.12	49.5 µM	5.29E-5		16	1.87E-5
11	SGTC_3340	9146634	4.10	11.1 µM	5.77E-5		9	2.06E-5
12	SGTC_771	0250-0039	3.94	27.3 µM	1.06E-4		14	4.07E-5
13	SGTC_2609	2,3,4-trihydroxy-4'-ethoxybenzophenone	3.86	70.0 µM	1.40E-4	redox potentiating	78	5.56E-5
14	SGTC_2429	5902844	3.80	64.4 µM	1.77E-4		30	7.23E-5
15	SGTC_660	4031-1742	3.80	102.0 µM	1.78E-4	redox potentiating	54	7.27E-5
16	SGTC_1835	st008666	3.70	49.2 µM	2.52E-4		30	1.07E-4
17	SGTC_1887	st057648	3.70	70.8 µM	2.53E-4		24	1.08E-4
18	SGTC_946	3434-1334	3.69	87.4 µM	2.59E-4		38	1.10E-4
19	SGTC_1607	st000305	3.64	50.6 µM	3.12E-4		19	1.36E-4
20	SGTC_202	3594-0513	3.64	34.8 µM	3.16E-4	PDR1	15	1.38E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.201	7.42E-32	YDR516C	EMI2	hom	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.169	8.25E-23	YIL039W	TED1	hom	Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.166	3.09E-22	YOL100W	PKH2	hom	Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.165	5.89E-22	YPL208W	RKM1	hom	SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.151	1.63E-18	YLR048W	RPS0B	hom	Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.145	3.55E-17	YGR214W	RPS0A	hom	Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
0.140	4.14E-16	YGL250W	RMR1	hom	Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.137	1.79E-15	YLR367W	RPS22B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.133	1.08E-14	YGL251C	HFM1	hom	Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.128	9.70E-14	YDR447C	RPS17B	hom	Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.126	2.90E-13	YGL168W	HUR1	hom	Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
0.122	1.42E-12	YPL131W	RPL5	het	Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
0.118	6.62E-12	YBR191W	RPL21A	hom	Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.115	2.50E-11	YBR162C	TOS1	hom	Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.111	9.62E-11	YDR281C	PHM6	hom	Protein of unknown function, expression is regulated by phosphate levels