YGL244W / RTF1

Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL244W / RTF1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.30|P-value:5.12E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:8.38|P-value:7.42E-11 ID:SGTC_103|Compound:0844-0104|FD-Score:-7.85|P-value:8.80E-10 ID:SGTC_158|Compound:k038-0087|FD-Score:5.44|P-value:1.60E-5 ID:SGTC_161|Compound:4065-0411|FD-Score:5.76|P-value:5.33E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.58|P-value:2.30E-4 ID:SGTC_270|Compound:tamoxifen|FD-Score:4.78|P-value:1.29E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:4.11|P-value:8.49E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:4.14|P-value:7.71E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.52|P-value:2.60E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.08|P-value:9.03E-4 ID:SGTC_398|Compound:bronopol|FD-Score:4.84|P-value:1.08E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:12.80|P-value:4.22E-23 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.97|P-value:6.88E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.13|P-value:4.42E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:4.11|P-value:8.46E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.24|P-value:6.01E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:7.13|P-value:2.44E-8 ID:SGTC_562|Compound:3772-2733|FD-Score:-4.25|P-value:5.57E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-6.10|P-value:1.44E-6 ID:SGTC_601|Compound:0986-0029|FD-Score:4.71|P-value:1.60E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:4.49|P-value:2.96E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.64|P-value:1.85E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:6.84|P-value:8.30E-8 ID:SGTC_767|Compound:0929-0023|FD-Score:5.09|P-value:5.02E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:6.83|P-value:8.64E-8 ID:SGTC_796|Compound:1234-0020|FD-Score:5.48|P-value:1.39E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:-4.66|P-value:1.77E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.26|P-value:5.63E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:4.46|P-value:3.22E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:4.91|P-value:8.72E-5 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:-4.03|P-value:9.95E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:4.85|P-value:1.05E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-6.43|P-value:4.08E-7 ID:SGTC_1167|Compound:k781-0610|FD-Score:5.03|P-value:6.02E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.85|P-value:1.05E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:5.91|P-value:3.10E-6 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.49|P-value:2.96E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:4.34|P-value:4.52E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:-4.60|P-value:2.10E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:6.99|P-value:4.55E-8 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.53|P-value:2.68E-4 ID:SGTC_1717|Compound:st033143|FD-Score:4.33|P-value:4.72E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.67|P-value:1.77E-4 ID:SGTC_1754|Compound:st042065|FD-Score:7.25|P-value:1.46E-8 ID:SGTC_1759|Compound:st043059|FD-Score:6.64|P-value:1.89E-7 ID:SGTC_1780|Compound:st038448|FD-Score:-4.47|P-value:3.03E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.34|P-value:4.53E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.55|P-value:2.56E-4 ID:SGTC_1801|Compound:st050089|FD-Score:4.50|P-value:2.91E-4 ID:SGTC_1803|Compound:st050068|FD-Score:5.92|P-value:2.99E-6 ID:SGTC_1909|Compound:st060837|FD-Score:8.03|P-value:3.97E-10 ID:SGTC_1917|Compound:st066507|FD-Score:4.07|P-value:9.43E-4 ID:SGTC_1985|Compound:st071382|FD-Score:-4.63|P-value:1.94E-4 ID:SGTC_1991|Compound:st073000|FD-Score:8.08|P-value:3.17E-10 ID:SGTC_2006|Compound:4011487|FD-Score:-4.33|P-value:4.44E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-8.38|P-value:6.61E-11 ID:SGTC_2077|Compound:5212015|FD-Score:5.76|P-value:5.31E-6 ID:SGTC_2082|Compound:5219693|FD-Score:6.41|P-value:4.66E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:8.16|P-value:2.21E-10 ID:SGTC_2258|Compound:7960471|FD-Score:-4.30|P-value:4.84E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-4.22|P-value:6.04E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-4.99|P-value:6.47E-5 ID:SGTC_2332|Compound:7982454|FD-Score:4.20|P-value:6.56E-4 ID:SGTC_2351|Compound:9026858|FD-Score:4.17|P-value:7.18E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.02|P-value:6.23E-5 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:6.71|P-value:1.42E-7 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:-5.47|P-value:1.37E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:6.40|P-value:4.84E-7 ID:SGTC_2767|Compound:sibutramine|FD-Score:4.41|P-value:3.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-8.56|P-value:2.73E-11 ID:SGTC_3175|Compound:9106517|FD-Score:-4.55|P-value:2.39E-4 ID:SGTC_3181|Compound:9108277|FD-Score:-4.10|P-value:8.37E-4 ID:SGTC_3351|Compound:9150312|FD-Score:4.12|P-value:8.16E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.30|P-value:5.12E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:8.38|P-value:7.42E-11 ID:SGTC_103|Compound:0844-0104|FD-Score:-7.85|P-value:8.80E-10 ID:SGTC_158|Compound:k038-0087|FD-Score:5.44|P-value:1.60E-5 ID:SGTC_161|Compound:4065-0411|FD-Score:5.76|P-value:5.33E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.58|P-value:2.30E-4 ID:SGTC_270|Compound:tamoxifen|FD-Score:4.78|P-value:1.29E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:4.11|P-value:8.49E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:4.14|P-value:7.71E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.52|P-value:2.60E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.08|P-value:9.03E-4 ID:SGTC_398|Compound:bronopol|FD-Score:4.84|P-value:1.08E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:12.80|P-value:4.22E-23 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.97|P-value:6.88E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.13|P-value:4.42E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:4.11|P-value:8.46E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.24|P-value:6.01E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:7.13|P-value:2.44E-8 ID:SGTC_562|Compound:3772-2733|FD-Score:-4.25|P-value:5.57E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-6.10|P-value:1.44E-6 ID:SGTC_601|Compound:0986-0029|FD-Score:4.71|P-value:1.60E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:4.49|P-value:2.96E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.64|P-value:1.85E-4 ID:SGTC_727|Compound:3032-0951|FD-Score:6.84|P-value:8.30E-8 ID:SGTC_767|Compound:0929-0023|FD-Score:5.09|P-value:5.02E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:6.83|P-value:8.64E-8 ID:SGTC_796|Compound:1234-0020|FD-Score:5.48|P-value:1.39E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:-4.66|P-value:1.77E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.26|P-value:5.63E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:4.46|P-value:3.22E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:4.91|P-value:8.72E-5 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:-4.03|P-value:9.95E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:4.85|P-value:1.05E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-6.43|P-value:4.08E-7 ID:SGTC_1167|Compound:k781-0610|FD-Score:5.03|P-value:6.02E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.85|P-value:1.05E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:5.91|P-value:3.10E-6 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.49|P-value:2.96E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:4.34|P-value:4.52E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:-4.60|P-value:2.10E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:6.99|P-value:4.55E-8 ID:SGTC_1459|Compound:k035-0031|FD-Score:4.53|P-value:2.68E-4 ID:SGTC_1717|Compound:st033143|FD-Score:4.33|P-value:4.72E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.67|P-value:1.77E-4 ID:SGTC_1754|Compound:st042065|FD-Score:7.25|P-value:1.46E-8 ID:SGTC_1759|Compound:st043059|FD-Score:6.64|P-value:1.89E-7 ID:SGTC_1780|Compound:st038448|FD-Score:-4.47|P-value:3.03E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.34|P-value:4.53E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.55|P-value:2.56E-4 ID:SGTC_1801|Compound:st050089|FD-Score:4.50|P-value:2.91E-4 ID:SGTC_1803|Compound:st050068|FD-Score:5.92|P-value:2.99E-6 ID:SGTC_1909|Compound:st060837|FD-Score:8.03|P-value:3.97E-10 ID:SGTC_1917|Compound:st066507|FD-Score:4.07|P-value:9.43E-4 ID:SGTC_1985|Compound:st071382|FD-Score:-4.63|P-value:1.94E-4 ID:SGTC_1991|Compound:st073000|FD-Score:8.08|P-value:3.17E-10 ID:SGTC_2006|Compound:4011487|FD-Score:-4.33|P-value:4.44E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-8.38|P-value:6.61E-11 ID:SGTC_2077|Compound:5212015|FD-Score:5.76|P-value:5.31E-6 ID:SGTC_2082|Compound:5219693|FD-Score:6.41|P-value:4.66E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:8.16|P-value:2.21E-10 ID:SGTC_2258|Compound:7960471|FD-Score:-4.30|P-value:4.84E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-4.22|P-value:6.04E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-4.99|P-value:6.47E-5 ID:SGTC_2332|Compound:7982454|FD-Score:4.20|P-value:6.56E-4 ID:SGTC_2351|Compound:9026858|FD-Score:4.17|P-value:7.18E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.02|P-value:6.23E-5 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:6.71|P-value:1.42E-7 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:-5.47|P-value:1.37E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:6.40|P-value:4.84E-7 ID:SGTC_2767|Compound:sibutramine|FD-Score:4.41|P-value:3.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-8.56|P-value:2.73E-11 ID:SGTC_3175|Compound:9106517|FD-Score:-4.55|P-value:2.39E-4 ID:SGTC_3181|Compound:9108277|FD-Score:-4.10|P-value:8.37E-4 ID:SGTC_3351|Compound:9150312|FD-Score:4.12|P-value:8.16E-4

Top fitness defect scores for YGL244W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_439 amiodarone 12.80 34.4 µM 4.22E-23 NEO1-PIK1 9 4.60E-38
2 SGTC_86 0417-1630 8.38 471.9 µM 7.42E-11 ubiquinone biosynthesis & proteasome 55 2.75E-17
3 SGTC_2250 alverine citrate 8.16 130.0 µM 2.21E-10 19 1.74E-16
4 SGTC_1991 st073000 8.08 8.9 µM 3.17E-10 NEO1 15 3.21E-16
5 SGTC_1909 st060837 8.03 51.5 µM 3.97E-10 11 4.69E-16
6 SGTC_1754 st042065 7.25 16.1 µM 1.46E-8 NEO1-PIK1 66 2.06E-13
7 SGTC_556 4058-0063 7.13 51.5 µM 2.44E-8 3 4.90E-13
8 SGTC_1360 1598-0022 6.99 39.8 µM 4.55E-8 ERG2 4 1.40E-12
9 SGTC_727 3032-0951 6.84 202.0 µM 8.30E-8 4 3.86E-12
10 SGTC_794 k038-0031 6.83 316.0 µM 8.64E-8 ubiquinone biosynthesis & proteasome 43 4.14E-12
11 SGTC_2658 polymyxin b sulfate 6.71 77.0 µM 1.42E-7 NEO1 14 9.53E-12
12 SGTC_1759 st043059 6.64 70.0 µM 1.89E-7 amide catabolism 2 1.55E-11
13 SGTC_2082 5219693 6.41 150.0 µM 4.66E-7 RNA processing & uracil transport 4 7.07E-11
14 SGTC_2756 carbadox 6.40 75.1 µM 4.84E-7 14 7.53E-11
15 SGTC_1803 st050068 5.92 52.4 µM 2.99E-6 NEO1-PIK1 86 1.61E-9
16 SGTC_1252 0493-0875 5.91 34.8 µM 3.10E-6 5 1.70E-9
17 SGTC_2077 5212015 5.76 200.0 µM 5.31E-6 26 4.20E-9
18 SGTC_161 4065-0411 5.76 162.2 µM 5.33E-6 29 4.24E-9
19 SGTC_796 1234-0020 5.48 70.7 µM 1.39E-5 SWF1 & branched chain AA biosynthesis 8 2.10E-8
20 SGTC_158 k038-0087 5.44 26.4 µM 1.60E-5 ubiquinone biosynthesis & proteasome 81 2.67E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 3.35E-39 YLR418C CDC73 hom Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
0.199 2.55E-31 YDL074C BRE1 hom E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control
0.190 1.16E-28 YOL145C CTR9 hom Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
0.184 6.39E-27 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.182 1.90E-26 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.173 4.94E-24 YLR015W BRE2 hom Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
0.164 1.11E-21 YJL169W_d YJL169W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
0.158 3.72E-20 YBR021W FUR4 hom Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
0.154 2.84E-19 YDR469W SDC1 hom Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
0.151 1.75E-18 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.147 1.29E-17 YPL005W AEP3 hom Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA
0.147 1.40E-17 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.146 2.31E-17 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.145 2.63E-17 YLR085C ARP6 hom Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.143 1.09E-16 YEL003W GIM4 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it