YGL245W / GUS1

Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL245W / GUS1

Click on Significant Values for Screen Details ID:SGTC_167|Compound:3672-0051|FD-Score:-3.96|P-value:3.79E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:-3.23|P-value:6.10E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.62|P-value:1.64E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.19|P-value:7.83E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.34|P-value:4.74E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.27|P-value:5.97E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.76|P-value:9.52E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.18|P-value:8.10E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-3.50|P-value:2.33E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-4.81|P-value:7.86E-7 ID:SGTC_1919|Compound:st067113|FD-Score:-6.54|P-value:3.40E-11 ID:SGTC_2002|Compound:4023503|FD-Score:4.53|P-value:3.55E-6 ID:SGTC_2008|Compound:4012253|FD-Score:-7.26|P-value:2.21E-13 ID:SGTC_2010|Compound:4012422|FD-Score:-6.26|P-value:2.12E-10 ID:SGTC_2440|Compound:5628896|FD-Score:3.13|P-value:9.67E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.14|P-value:9.21E-4 ID:SGTC_2815|Compound:7968870|FD-Score:5.11|P-value:2.03E-7 ID:SGTC_2850|Compound:9015429|FD-Score:3.20|P-value:7.60E-4 ID:SGTC_2857|Compound:9024691|FD-Score:5.02|P-value:3.14E-7 ID:SGTC_2885|Compound:9057732|FD-Score:3.38|P-value:4.04E-4 ID:SGTC_3092|Compound:9117248|FD-Score:3.29|P-value:5.56E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.96|P-value:3.79E-5 ID:SGTC_196|Compound:k015-0023|FD-Score:-3.23|P-value:6.10E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.62|P-value:1.64E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.19|P-value:7.83E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.34|P-value:4.74E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.27|P-value:5.97E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.76|P-value:9.52E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.18|P-value:8.10E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-3.50|P-value:2.33E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-4.81|P-value:7.86E-7 ID:SGTC_1919|Compound:st067113|FD-Score:-6.54|P-value:3.40E-11 ID:SGTC_2002|Compound:4023503|FD-Score:4.53|P-value:3.55E-6 ID:SGTC_2008|Compound:4012253|FD-Score:-7.26|P-value:2.21E-13 ID:SGTC_2010|Compound:4012422|FD-Score:-6.26|P-value:2.12E-10 ID:SGTC_2440|Compound:5628896|FD-Score:3.13|P-value:9.67E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.14|P-value:9.21E-4 ID:SGTC_2815|Compound:7968870|FD-Score:5.11|P-value:2.03E-7 ID:SGTC_2850|Compound:9015429|FD-Score:3.20|P-value:7.60E-4 ID:SGTC_2857|Compound:9024691|FD-Score:5.02|P-value:3.14E-7 ID:SGTC_2885|Compound:9057732|FD-Score:3.38|P-value:4.04E-4 ID:SGTC_3092|Compound:9117248|FD-Score:3.29|P-value:5.56E-4

Top fitness defect scores for YGL245W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2815 7968870 5.11 71.4 µM 2.03E-7 3 1.65E-7 0.68
2 SGTC_2857 9024691 5.02 58.4 µM 3.14E-7 2 2.57E-7 1.03
3 SGTC_2002 4023503 4.53 131.0 µM 3.55E-6 mitochondrial processes 5 2.99E-6 0.16
4 SGTC_1278 0874-0585 3.76 172.0 µM 9.52E-5 7 8.34E-5 0.00
5 SGTC_359 0081-0058 3.62 15.9 µM 1.64E-4 60S ribosome export 17 1.45E-4 0.03
6 SGTC_2885 9057732 3.38 52.0 µM 4.04E-4 6 3.61E-4 0.04
7 SGTC_877 0771-0151 3.34 33.1 µM 4.74E-4 4 4.24E-4 0.07
8 SGTC_3092 9117248 3.29 49.5 µM 5.56E-4 cell wall signaling 3 4.98E-4 0.08
9 SGTC_1122 capsaicin 3.27 818.6 nM 5.97E-4 11 5.36E-4 0.08
10 SGTC_2850 9015429 3.20 58.4 µM 7.60E-4 16 6.84E-4 0.13
11 SGTC_752 1896-0090 3.19 130.0 µM 7.83E-4 TSC3-RPN4 12 7.05E-4 0.05
12 SGTC_1348 1493-0373 3.18 93.8 µM 8.10E-4 11 7.30E-4 0.19
13 SGTC_2813 7971799 3.14 71.4 µM 9.21E-4 20 8.31E-4 0.26
14 SGTC_2440 5628896 3.13 157.8 µM 9.67E-4 13 8.73E-4 0.28
15 SGTC_1975 st070967 3.10 30.8 µM 0.00106 24 9.54E-4 0.01
16 SGTC_162 k030-0015 3.09 37.8 µM 0.00110 iron homeostasis 9 9.99E-4 0.02
17 SGTC_3324 9138759 3.07 60.8 µM 0.00120 12 0.00108 0.01
18 SGTC_3131 9127911 3.01 49.5 µM 0.00143 16 0.00130 0.22
19 SGTC_1206 3978-0017 2.98 132.0 µM 0.00158 ERG2 20 0.00143 0.16
20 SGTC_191 3914-0123 2.88 66.5 µM 0.00216 60S ribosome export 15 0.00197 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 1.71E-19 YGR042W YGR042W hom Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
0.150 2.00E-18 YDR277C MTH1 hom Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
0.144 5.07E-17 YGR018C_p YGR018C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
0.144 5.70E-17 YOR314W_d YOR314W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.144 5.75E-17 YLR280C_d YLR280C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.135 4.04E-15 YPR013C_p CMR3_p hom Putative zinc finger protein; YPR013C is not an essential gene
0.135 4.62E-15 YGL145W TIP20 het Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
0.121 2.15E-12 YNL039W BDP1 het Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
0.114 3.72E-11 YEL040W UTR2 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
0.109 2.56E-10 YHR190W ERG9 het Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
0.106 8.55E-10 YDR258C HSP78 hom Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates
0.104 1.31E-9 YDL091C UBX3 hom Subunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10
0.100 5.76E-9 YGL157W ARI1 hom NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
0.100 6.30E-9 YDL055C PSA1 het GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
0.100 7.15E-9 YDL052C SLC1 hom 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes