YGL250W / RMR1

Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL250W / RMR1

Click on Significant Values for Screen Details ID:SGTC_159|Compound:3970-0959|FD-Score:3.24|P-value:3.67E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.49|P-value:1.40E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:3.03|P-value:8.09E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.29|P-value:3.97E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:4.97|P-value:1.11E-7 ID:SGTC_268|Compound:camptothecin|FD-Score:3.53|P-value:1.19E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.19|P-value:4.49E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:-3.39|P-value:2.16E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.27|P-value:4.51E-6 ID:SGTC_422|Compound:cantharidin|FD-Score:2.98|P-value:9.35E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:3.25|P-value:3.60E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.75|P-value:4.87E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:3.14|P-value:5.30E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.42|P-value:1.84E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:10.50|P-value:4.25E-28 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.32|P-value:2.68E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.09|P-value:6.64E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.01|P-value:8.57E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.76|P-value:4.56E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.10|P-value:6.18E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:4.82|P-value:2.53E-7 ID:SGTC_1519|Compound:esterone|FD-Score:3.25|P-value:3.49E-4 ID:SGTC_1611|Compound:st001856|FD-Score:2.99|P-value:9.15E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.22|P-value:4.03E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-4.11|P-value:9.40E-6 ID:SGTC_1800|Compound:st050079|FD-Score:-3.71|P-value:5.65E-5 ID:SGTC_1817|Compound:st052116|FD-Score:-3.07|P-value:7.10E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.05|P-value:1.24E-5 ID:SGTC_1859|Compound:st055998|FD-Score:2.99|P-value:9.05E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.26|P-value:3.46E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-3.79|P-value:4.01E-5 ID:SGTC_2111|Compound:5486399|FD-Score:-3.90|P-value:2.53E-5 ID:SGTC_2121|Compound:5257820|FD-Score:4.23|P-value:5.27E-6 ID:SGTC_2133|Compound:5320837|FD-Score:-3.69|P-value:6.34E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.12|P-value:9.19E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.79|P-value:3.99E-5 ID:SGTC_2997|Compound:9067075|FD-Score:3.73|P-value:5.09E-5 ID:SGTC_3159|Compound:9100096|FD-Score:-4.31|P-value:3.66E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:3.24|P-value:3.67E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.49|P-value:1.40E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:3.03|P-value:8.09E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.29|P-value:3.97E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:4.97|P-value:1.11E-7 ID:SGTC_268|Compound:camptothecin|FD-Score:3.53|P-value:1.19E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.19|P-value:4.49E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:-3.39|P-value:2.16E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.27|P-value:4.51E-6 ID:SGTC_422|Compound:cantharidin|FD-Score:2.98|P-value:9.35E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:3.25|P-value:3.60E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.75|P-value:4.87E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:3.14|P-value:5.30E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.42|P-value:1.84E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:10.50|P-value:4.25E-28 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.32|P-value:2.68E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.09|P-value:6.64E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.01|P-value:8.57E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.76|P-value:4.56E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.10|P-value:6.18E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:4.82|P-value:2.53E-7 ID:SGTC_1519|Compound:esterone|FD-Score:3.25|P-value:3.49E-4 ID:SGTC_1611|Compound:st001856|FD-Score:2.99|P-value:9.15E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.22|P-value:4.03E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-4.11|P-value:9.40E-6 ID:SGTC_1800|Compound:st050079|FD-Score:-3.71|P-value:5.65E-5 ID:SGTC_1817|Compound:st052116|FD-Score:-3.07|P-value:7.10E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.05|P-value:1.24E-5 ID:SGTC_1859|Compound:st055998|FD-Score:2.99|P-value:9.05E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.26|P-value:3.46E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-3.79|P-value:4.01E-5 ID:SGTC_2111|Compound:5486399|FD-Score:-3.90|P-value:2.53E-5 ID:SGTC_2121|Compound:5257820|FD-Score:4.23|P-value:5.27E-6 ID:SGTC_2133|Compound:5320837|FD-Score:-3.69|P-value:6.34E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.12|P-value:9.19E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.79|P-value:3.99E-5 ID:SGTC_2997|Compound:9067075|FD-Score:3.73|P-value:5.09E-5 ID:SGTC_3159|Compound:9100096|FD-Score:-4.31|P-value:3.66E-6

Top fitness defect scores for YGL250W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_800 3448-7362 10.50 155.0 µM 4.25E-28 translation 4 4.53E-26
2 SGTC_214 4373-3496 4.97 70.0 µM 1.11E-7 29 3.28E-7
3 SGTC_1401 3807-4679 4.82 7.6 µM 2.53E-7 tubulin folding & SWR complex 9 7.05E-7
4 SGTC_212 3807-4682 4.29 38.8 µM 3.97E-6 tubulin folding & SWR complex 53 9.00E-6
5 SGTC_2121 5257820 4.23 149.6 µM 5.27E-6 7 1.17E-5
6 SGTC_2322 7413319 3.79 38.2 µM 3.99E-5 ERAD & cell cycle 162 7.63E-5
7 SGTC_2997 9067075 3.73 71.4 µM 5.09E-5 unfolded protein response 61 9.55E-5
8 SGTC_268 camptothecin 3.53 39.6 µM 1.19E-4 DNA damage response 93 2.09E-4
9 SGTC_168 3970-0862 3.49 530.5 µM 1.40E-4 158 2.44E-4
10 SGTC_660 4031-1742 3.42 102.0 µM 1.84E-4 redox potentiating 71 3.14E-4
11 SGTC_1104 tacrolimus 3.32 29.8 µM 2.68E-4 57 4.45E-4
12 SGTC_1982 st072467 3.26 71.9 µM 3.46E-4 66 5.64E-4
13 SGTC_1519 esterone 3.25 74.0 µM 3.49E-4 DNA intercalators 45 5.69E-4
14 SGTC_432 NS 1619 3.25 61.3 µM 3.60E-4 58 5.85E-4
15 SGTC_159 3970-0959 3.24 50.0 µM 3.67E-4 135 5.96E-4
16 SGTC_655 k060-0012 3.14 42.0 µM 5.30E-4 44 8.38E-4
17 SGTC_1391 3442-0225 3.10 151.0 µM 6.18E-4 41 9.66E-4
18 SGTC_209 4077-0173 3.03 175.6 µM 8.09E-4 25 0.00124
19 SGTC_1290 0986-0249 3.01 10.2 µM 8.57E-4 DNA intercalators 50 0.00131
20 SGTC_1859 st055998 2.99 20.0 µM 9.05E-4 60 0.00138

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.203 1.93E-32 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.194 1.05E-29 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.194 1.07E-29 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.180 6.24E-26 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.180 6.64E-26 YBR162C TOS1 hom Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.169 8.48E-23 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.161 7.54E-21 YLR287C-A RPS30A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
0.154 3.13E-19 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.153 3.79E-19 YER151C UBP3 hom Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
0.153 5.41E-19 YOR141C ARP8 hom Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity
0.144 4.79E-17 YJL069C UTP18 het Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.142 1.11E-16 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.142 1.44E-16 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.142 1.56E-16 YBR267W REI1 hom Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.140 4.14E-16 YGL236C MTO1 hom Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants