YGL251C / HFM1

Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL251C / HFM1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.01|P-value:1.26E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.64|P-value:4.54E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:3.57|P-value:5.71E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.63|P-value:4.52E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.42|P-value:9.05E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-3.47|P-value:7.71E-4 ID:SGTC_470|Compound:aa-861|FD-Score:5.86|P-value:4.51E-8 ID:SGTC_528|Compound:3492-0019|FD-Score:-4.39|P-value:3.09E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:4.24|P-value:5.55E-5 ID:SGTC_674|Compound:0498-0004|FD-Score:4.35|P-value:3.68E-5 ID:SGTC_739|Compound:1486-1183|FD-Score:3.45|P-value:8.33E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:4.61|P-value:1.30E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:4.11|P-value:9.07E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.67|P-value:4.06E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:3.78|P-value:2.86E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-4.52|P-value:1.83E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:-5.15|P-value:1.26E-6 ID:SGTC_955|Compound:1216-0110|FD-Score:5.69|P-value:1.07E-7 ID:SGTC_960|Compound:1222-0186|FD-Score:4.86|P-value:4.77E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:-3.98|P-value:1.40E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:-3.93|P-value:1.67E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.57|P-value:5.61E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:-4.10|P-value:9.22E-5 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.72|P-value:3.38E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.95|P-value:1.55E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.43|P-value:8.92E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.81|P-value:2.55E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-4.03|P-value:1.15E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-4.23|P-value:5.59E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-4.02|P-value:1.21E-4 ID:SGTC_1817|Compound:st052116|FD-Score:-3.68|P-value:3.85E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.34|P-value:3.73E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.96|P-value:1.52E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.44|P-value:8.33E-4 ID:SGTC_2004|Compound:4023728|FD-Score:-3.68|P-value:3.94E-4 ID:SGTC_2053|Compound:5265679|FD-Score:5.62|P-value:1.48E-7 ID:SGTC_2056|Compound:5235370|FD-Score:-3.49|P-value:7.16E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.78|P-value:2.81E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-4.08|P-value:9.81E-5 ID:SGTC_2332|Compound:7982454|FD-Score:3.58|P-value:5.53E-4 ID:SGTC_2457|Compound:5731572|FD-Score:4.92|P-value:3.64E-6 ID:SGTC_2461|Compound:5755302|FD-Score:3.68|P-value:3.97E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.66|P-value:4.20E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.40|P-value:9.48E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.84|P-value:2.30E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:5.42|P-value:3.93E-7 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.06|P-value:1.04E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:3.96|P-value:1.51E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:3.76|P-value:3.06E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:-3.48|P-value:7.31E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.48|P-value:7.48E-4 ID:SGTC_3003|Compound:9075612|FD-Score:-4.23|P-value:5.69E-5 ID:SGTC_3037|Compound:9091026|FD-Score:-3.53|P-value:6.36E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-3.57|P-value:5.55E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.01|P-value:1.26E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.64|P-value:4.54E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:3.57|P-value:5.71E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.63|P-value:4.52E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.42|P-value:9.05E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-3.47|P-value:7.71E-4 ID:SGTC_470|Compound:aa-861|FD-Score:5.86|P-value:4.51E-8 ID:SGTC_528|Compound:3492-0019|FD-Score:-4.39|P-value:3.09E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:4.24|P-value:5.55E-5 ID:SGTC_674|Compound:0498-0004|FD-Score:4.35|P-value:3.68E-5 ID:SGTC_739|Compound:1486-1183|FD-Score:3.45|P-value:8.33E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:4.61|P-value:1.30E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:4.11|P-value:9.07E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.67|P-value:4.06E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:3.78|P-value:2.86E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-4.52|P-value:1.83E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:-5.15|P-value:1.26E-6 ID:SGTC_955|Compound:1216-0110|FD-Score:5.69|P-value:1.07E-7 ID:SGTC_960|Compound:1222-0186|FD-Score:4.86|P-value:4.77E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:-3.98|P-value:1.40E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:-3.93|P-value:1.67E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.57|P-value:5.61E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:-4.10|P-value:9.22E-5 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.72|P-value:3.38E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.95|P-value:1.55E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.43|P-value:8.92E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.81|P-value:2.55E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-4.03|P-value:1.15E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-4.23|P-value:5.59E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-4.02|P-value:1.21E-4 ID:SGTC_1817|Compound:st052116|FD-Score:-3.68|P-value:3.85E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-4.34|P-value:3.73E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.96|P-value:1.52E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.44|P-value:8.33E-4 ID:SGTC_2004|Compound:4023728|FD-Score:-3.68|P-value:3.94E-4 ID:SGTC_2053|Compound:5265679|FD-Score:5.62|P-value:1.48E-7 ID:SGTC_2056|Compound:5235370|FD-Score:-3.49|P-value:7.16E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.78|P-value:2.81E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-4.08|P-value:9.81E-5 ID:SGTC_2332|Compound:7982454|FD-Score:3.58|P-value:5.53E-4 ID:SGTC_2457|Compound:5731572|FD-Score:4.92|P-value:3.64E-6 ID:SGTC_2461|Compound:5755302|FD-Score:3.68|P-value:3.97E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.66|P-value:4.20E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.40|P-value:9.48E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.84|P-value:2.30E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:5.42|P-value:3.93E-7 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.06|P-value:1.04E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:3.96|P-value:1.51E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:3.76|P-value:3.06E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:-3.48|P-value:7.31E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.48|P-value:7.48E-4 ID:SGTC_3003|Compound:9075612|FD-Score:-4.23|P-value:5.69E-5 ID:SGTC_3037|Compound:9091026|FD-Score:-3.53|P-value:6.36E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-3.57|P-value:5.55E-4

Top fitness defect scores for YGL251C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_470 aa-861 5.86 153.0 µM 4.51E-8 sphingolipid biosynthesis & PDR1 24 2.28E-9
2 SGTC_955 1216-0110 5.69 33.0 µM 1.07E-7 sphingolipid biosynthesis & PDR1 34 6.37E-9
3 SGTC_2053 5265679 5.62 138.0 µM 1.48E-7 2 9.42E-9
4 SGTC_2630 antimycin a 5.42 100.0 µM 3.93E-7 mitochondrial processes 16 3.03E-8
5 SGTC_2457 5731572 4.92 11.7 µM 3.64E-6 8 4.33E-7
6 SGTC_960 1222-0186 4.86 7.7 µM 4.77E-6 15 5.98E-7
7 SGTC_749 1000-0129 4.61 128.0 µM 1.30E-5 amide catabolism 7 1.98E-6
8 SGTC_674 0498-0004 4.35 451.0 µM 3.68E-5 heme requiring 16 6.85E-6
9 SGTC_625 k213-0058 4.24 121.0 µM 5.55E-5 translation 86 1.12E-5
10 SGTC_750 4073-0104 4.11 132.0 µM 9.07E-5 10 2.01E-5
11 SGTC_2641 levomentholum 3.96 100.0 µM 1.51E-4 8 3.68E-5
12 SGTC_1893 st059353 3.96 36.1 µM 1.52E-4 25 3.71E-5
13 SGTC_2629 bisabolol 3.84 100.0 µM 2.30E-4 23 6.09E-5
14 SGTC_900 1112-0045 3.78 141.0 µM 2.86E-4 NEO1 27 7.87E-5
15 SGTC_2648 atorvastatin 3.76 78.7 µM 3.06E-4 azole & statin 24 8.52E-5
16 SGTC_2461 5755302 3.68 26.1 µM 3.97E-4 46 1.16E-4
17 SGTC_832 4083-0019 3.67 164.0 µM 4.06E-4 translation 117 1.19E-4
18 SGTC_2489 cantharidin 3.66 73.4 µM 4.20E-4 cell wall signaling 57 1.24E-4
19 SGTC_62 0180-0423 3.64 3.2 µM 4.54E-4 60S ribosome export 28 1.36E-4
20 SGTC_2332 7982454 3.58 138.9 µM 5.53E-4 68 1.72E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.226 5.62E-40 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.204 5.38E-33 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.170 4.47E-23 YIL039W TED1 hom Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.164 1.69E-21 YBR162C TOS1 hom Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.157 4.92E-20 YOL100W PKH2 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.137 1.68E-15 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.133 1.08E-14 YGL236C MTO1 hom Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
0.132 1.62E-14 YDR281C PHM6 hom Protein of unknown function, expression is regulated by phosphate levels
0.120 2.98E-12 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.119 4.23E-12 YGR114C_d YGR114C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
0.117 9.13E-12 YIL103W DPH1 hom Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
0.115 2.83E-11 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.113 5.20E-11 YDR072C IPT1 hom Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
0.105 9.38E-10 YIL038C NOT3 hom Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
0.103 2.57E-9 YBR036C CSG2 hom Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress