YGL254W / FZF1

Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL254W / FZF1

Click on Significant Values for Screen Details ID:SGTC_813|Compound:k007-0158|FD-Score:-3.88|P-value:6.62E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.24|P-value:7.69E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.38|P-value:9.07E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.18|P-value:9.38E-4 ID:SGTC_1603|Compound:st002045|FD-Score:3.68|P-value:1.64E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.18|P-value:9.54E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.25|P-value:6.78E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.29|P-value:5.93E-4 ID:SGTC_1804|Compound:st050076|FD-Score:-3.15|P-value:9.41E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.50|P-value:3.16E-4 ID:SGTC_1944|Compound:st076371|FD-Score:5.28|P-value:1.22E-7 ID:SGTC_1950|Compound:st077106|FD-Score:-3.33|P-value:5.08E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.15|P-value:9.31E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:5.22|P-value:1.67E-7 ID:SGTC_1958|Compound:st076513|FD-Score:-3.83|P-value:7.91E-5 ID:SGTC_2056|Compound:5235370|FD-Score:-4.39|P-value:7.64E-6 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.23|P-value:7.10E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.48|P-value:3.41E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.74|P-value:1.81E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.25|P-value:7.66E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:-3.74|P-value:1.15E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:3.86|P-value:8.00E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-4.10|P-value:2.62E-5 ID:SGTC_2960|Compound:9079389|FD-Score:-3.18|P-value:8.50E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.10|P-value:3.10E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-7.50|P-value:7.87E-14 ID:SGTC_813|Compound:k007-0158|FD-Score:-3.88|P-value:6.62E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.24|P-value:7.69E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.38|P-value:9.07E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.18|P-value:9.38E-4 ID:SGTC_1603|Compound:st002045|FD-Score:3.68|P-value:1.64E-4 ID:SGTC_1605|Compound:st000082|FD-Score:3.18|P-value:9.54E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.25|P-value:6.78E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.29|P-value:5.93E-4 ID:SGTC_1804|Compound:st050076|FD-Score:-3.15|P-value:9.41E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.50|P-value:3.16E-4 ID:SGTC_1944|Compound:st076371|FD-Score:5.28|P-value:1.22E-7 ID:SGTC_1950|Compound:st077106|FD-Score:-3.33|P-value:5.08E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.15|P-value:9.31E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:5.22|P-value:1.67E-7 ID:SGTC_1958|Compound:st076513|FD-Score:-3.83|P-value:7.91E-5 ID:SGTC_2056|Compound:5235370|FD-Score:-4.39|P-value:7.64E-6 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.23|P-value:7.10E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.48|P-value:3.41E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.74|P-value:1.81E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.25|P-value:7.66E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:-3.74|P-value:1.15E-4 ID:SGTC_2521|Compound:angolensin|FD-Score:3.86|P-value:8.00E-5 ID:SGTC_2525|Compound:harmine|FD-Score:-4.10|P-value:2.62E-5 ID:SGTC_2960|Compound:9079389|FD-Score:-3.18|P-value:8.50E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.10|P-value:3.10E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-7.50|P-value:7.87E-14

Top fitness defect scores for YGL254W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1944 st076371 5.28 56.9 µM 1.22E-7 9 6.60E-8
2 SGTC_1955 wogonin 5.22 32.4 µM 1.67E-7 8 9.17E-8
3 SGTC_2513 baicalein 4.74 25.6 µM 1.81E-6 calcium & mitochondrial duress 13 1.09E-6
4 SGTC_996 3970-0799 4.38 1.8 µM 9.07E-6 68 5.82E-6
5 SGTC_2987 9018338 4.10 71.4 µM 3.10E-5 RPP1 & pyrimidine depletion 13 2.09E-5
6 SGTC_2521 angolensin 3.86 13.4 µM 8.00E-5 12 5.60E-5
7 SGTC_1603 st002045 3.68 67.8 µM 1.64E-4 TSC3-RPN4 20 1.19E-4
8 SGTC_1941 st076221 3.50 61.8 µM 3.16E-4 sphingolipid biosynthesis & PDR1 101 2.34E-4
9 SGTC_2453 5731132 3.48 22.2 µM 3.41E-4 33 2.53E-4
10 SGTC_2514 salinomycin 3.25 42.7 µM 7.66E-4 89 5.87E-4
11 SGTC_814 3342-0569 3.24 96.0 µM 7.69E-4 48 5.90E-4
12 SGTC_1125 tnp00248 3.18 1.3 µM 9.38E-4 81 7.26E-4
13 SGTC_1605 st000082 3.18 49.1 µM 9.54E-4 60S ribosome export 52 7.38E-4
14 SGTC_1451 4152-0210 3.02 104.0 µM 0.00159 68 0.00126
15 SGTC_2457 5731572 3.01 11.7 µM 0.00163 37 0.00129
16 SGTC_2135 5326629 3.00 150.4 µM 0.00169 RPP1 & pyrimidine depletion 77 0.00134
17 SGTC_2878 9043973 3.00 26.0 µM 0.00170 51 0.00135
18 SGTC_48 0382-0018 2.99 55.0 µM 0.00174 42 0.00138
19 SGTC_1361 1608-0041 2.97 5.6 µM 0.00186 RPP1 & pyrimidine depletion 54 0.00148
20 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 2.97 100.0 µM 0.00189 56 0.00150

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.238 2.61E-44 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.202 4.23E-32 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.180 7.13E-26 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.150 2.07E-18 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.143 7.77E-17 YGL242C_p YGL242C_p hom Putative protein of unknown function; deletion mutant is viable
0.142 1.50E-16 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.138 9.90E-16 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.131 2.21E-14 YBR085W AAC3 hom Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
0.129 7.03E-14 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.124 4.88E-13 YJL175W_d YJL175W_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor
0.121 2.40E-12 YPR194C OPT2 hom Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles
0.120 2.85E-12 YDR355C_d YDR355C_d het Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
0.120 3.37E-12 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.118 6.31E-12 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.116 1.96E-11 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix