YGR003W / CUL3

Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR003W / CUL3

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.05|P-value:4.69E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-2.90|P-value:7.62E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:3.43|P-value:9.82E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.20|P-value:2.56E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.48|P-value:7.80E-5 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-2.89|P-value:7.88E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:-3.32|P-value:1.42E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.14|P-value:3.20E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.68|P-value:3.18E-5 ID:SGTC_603|Compound:3794-0023|FD-Score:2.89|P-value:8.45E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.66|P-value:3.16E-5 ID:SGTC_614|Compound:0462-0418|FD-Score:-5.73|P-value:2.03E-10 ID:SGTC_616|Compound:4092-0450|FD-Score:-3.34|P-value:1.29E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:-2.94|P-value:6.41E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-2.93|P-value:6.72E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.00|P-value:5.51E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:2.88|P-value:8.68E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.52|P-value:6.41E-5 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.57|P-value:5.29E-5 ID:SGTC_1257|Compound:0669-0121|FD-Score:-4.22|P-value:1.97E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.16|P-value:2.99E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-2.97|P-value:5.86E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.37|P-value:1.22E-4 ID:SGTC_1540|Compound:guanosine|FD-Score:3.22|P-value:2.37E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:2.85|P-value:9.84E-4 ID:SGTC_1730|Compound:st033808|FD-Score:3.47|P-value:7.96E-5 ID:SGTC_1736|Compound:st036774|FD-Score:3.06|P-value:4.44E-4 ID:SGTC_1914|Compound:st062367|FD-Score:-2.84|P-value:9.45E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.37|P-value:1.13E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:-3.09|P-value:3.66E-4 ID:SGTC_2364|Compound:9055074|FD-Score:-3.59|P-value:4.45E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-4.29|P-value:1.38E-6 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-5.10|P-value:1.24E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.19|P-value:2.65E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.34|P-value:1.42E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.52|P-value:6.05E-5 ID:SGTC_2784|Compound:7567673|FD-Score:3.03|P-value:5.02E-4 ID:SGTC_2841|Compound:9004761|FD-Score:-2.98|P-value:5.67E-4 ID:SGTC_2919|Compound:7978188|FD-Score:2.96|P-value:6.50E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.70|P-value:2.84E-5 ID:SGTC_2985|Compound:9022375|FD-Score:4.03|P-value:5.84E-6 ID:SGTC_3147|Compound:9097027|FD-Score:-3.52|P-value:5.94E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.43|P-value:9.01E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-5.15|P-value:9.06E-9 ID:SGTC_65|Compound:0798-0905|FD-Score:3.05|P-value:4.69E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:-2.90|P-value:7.62E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:3.43|P-value:9.82E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:3.20|P-value:2.56E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.48|P-value:7.80E-5 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-2.89|P-value:7.88E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:-3.32|P-value:1.42E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.14|P-value:3.20E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.68|P-value:3.18E-5 ID:SGTC_603|Compound:3794-0023|FD-Score:2.89|P-value:8.45E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.66|P-value:3.16E-5 ID:SGTC_614|Compound:0462-0418|FD-Score:-5.73|P-value:2.03E-10 ID:SGTC_616|Compound:4092-0450|FD-Score:-3.34|P-value:1.29E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:-2.94|P-value:6.41E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-2.93|P-value:6.72E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.00|P-value:5.51E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:2.88|P-value:8.68E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.52|P-value:6.41E-5 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.57|P-value:5.29E-5 ID:SGTC_1257|Compound:0669-0121|FD-Score:-4.22|P-value:1.97E-6 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.16|P-value:2.99E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-2.97|P-value:5.86E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.37|P-value:1.22E-4 ID:SGTC_1540|Compound:guanosine|FD-Score:3.22|P-value:2.37E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:2.85|P-value:9.84E-4 ID:SGTC_1730|Compound:st033808|FD-Score:3.47|P-value:7.96E-5 ID:SGTC_1736|Compound:st036774|FD-Score:3.06|P-value:4.44E-4 ID:SGTC_1914|Compound:st062367|FD-Score:-2.84|P-value:9.45E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.37|P-value:1.13E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:-3.09|P-value:3.66E-4 ID:SGTC_2364|Compound:9055074|FD-Score:-3.59|P-value:4.45E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-4.29|P-value:1.38E-6 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-5.10|P-value:1.24E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.19|P-value:2.65E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.34|P-value:1.42E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.52|P-value:6.05E-5 ID:SGTC_2784|Compound:7567673|FD-Score:3.03|P-value:5.02E-4 ID:SGTC_2841|Compound:9004761|FD-Score:-2.98|P-value:5.67E-4 ID:SGTC_2919|Compound:7978188|FD-Score:2.96|P-value:6.50E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.70|P-value:2.84E-5 ID:SGTC_2985|Compound:9022375|FD-Score:4.03|P-value:5.84E-6 ID:SGTC_3147|Compound:9097027|FD-Score:-3.52|P-value:5.94E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.43|P-value:9.01E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-5.15|P-value:9.06E-9

Top fitness defect scores for YGR003W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2985 9022375 4.03 71.4 µM 5.84E-6 RPP1 & pyrimidine depletion 27 2.77E-5
2 SGTC_2976 9009005 3.70 19.5 µM 2.84E-5 34 1.06E-4
3 SGTC_552 k242-0005 3.68 476.0 µM 3.18E-5 23 1.17E-4
4 SGTC_1076 itraconazole 3.57 331.0 nM 5.29E-5 46 1.81E-4
5 SGTC_1075 aripiprazole 3.52 15.2 µM 6.41E-5 45 2.13E-4
6 SGTC_373 0844-0104 3.48 25.9 µM 7.80E-5 ERAD & cell cycle 71 2.52E-4
7 SGTC_1730 st033808 3.47 45.1 µM 7.96E-5 57 2.56E-4
8 SGTC_186 3455-3465 3.43 67.3 µM 9.82E-5 4 3.06E-4
9 SGTC_1310 1188-0297 3.37 994.0 nM 1.22E-4 azole & statin 49 3.70E-4
10 SGTC_2772 vinpocetine 3.34 57.1 µM 1.42E-4 44 4.20E-4
11 SGTC_1540 guanosine 3.22 70.6 µM 2.37E-4 24 6.49E-4
12 SGTC_370 0180-0345 3.20 38.7 µM 2.56E-4 DNA intercalators 31 6.92E-4
13 SGTC_2588 indole-3-carbinol 3.19 100.0 µM 2.65E-4 91 7.14E-4
14 SGTC_1259 0671-0132 3.16 43.0 µM 2.99E-4 61 7.91E-4
15 SGTC_415 dibenzyl ketone 3.14 100.0 µM 3.20E-4 36 8.38E-4
16 SGTC_1736 st036774 3.06 51.0 µM 4.44E-4 72 0.00111
17 SGTC_65 0798-0905 3.05 35.0 µM 4.69E-4 calcium & mitochondrial duress 159 0.00116
18 SGTC_2784 7567673 3.03 72.7 µM 5.02E-4 iron homeostasis 71 0.00123
19 SGTC_958 1222-0007 3.00 33.0 µM 5.51E-4 75 0.00133
20 SGTC_2919 7978188 2.96 46.4 µM 6.50E-4 70 0.00154

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.174 4.11E-24 YOR093C_p YOR093C_p hom Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents
0.146 1.53E-17 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.139 7.25E-16 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
0.135 3.94E-15 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.134 7.99E-15 YLR082C SRL2 hom Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
0.128 1.22E-13 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.126 2.75E-13 YLR290C_p YLR290C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene
0.125 3.82E-13 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.124 5.60E-13 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.123 7.79E-13 YOR062C_p YOR062C_p hom Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.120 3.02E-12 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.119 5.06E-12 YGL235W_p YGL235W_p hom Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.119 5.61E-12 YLR004C THI73 hom Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
0.111 1.16E-10 YGR093W_p YGR093W_p hom Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
0.103 2.51E-9 YLL006W-A_p YLL006W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR