YGR005C / TFG2

TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR005C / TFG2

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-4.28|P-value:1.95E-6 ID:SGTC_85|Compound:0868-0259|FD-Score:2.88|P-value:8.10E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:3.66|P-value:3.24E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:-3.01|P-value:6.19E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.80|P-value:1.71E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.22|P-value:2.16E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.13|P-value:3.87E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.87|P-value:8.36E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:3.69|P-value:2.80E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.44|P-value:8.38E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:7.24|P-value:1.66E-15 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.08|P-value:4.70E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.92|P-value:9.19E-6 ID:SGTC_535|Compound:2293-4129|FD-Score:3.83|P-value:1.44E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:3.28|P-value:1.69E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.94|P-value:8.48E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:2.91|P-value:7.19E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-7.86|P-value:1.04E-17 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.43|P-value:1.11E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.07|P-value:4.77E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.27|P-value:1.79E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.29|P-value:1.58E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:5.46|P-value:1.32E-9 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.05|P-value:4.32E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-5.94|P-value:7.35E-11 ID:SGTC_1493|Compound:4348-0038|FD-Score:5.05|P-value:1.90E-8 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:-3.65|P-value:4.20E-5 ID:SGTC_1673|Compound:st016611|FD-Score:5.11|P-value:1.27E-8 ID:SGTC_1712|Compound:st032288|FD-Score:3.80|P-value:1.67E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.54|P-value:5.49E-5 ID:SGTC_1804|Compound:st050076|FD-Score:-3.00|P-value:6.45E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.06|P-value:4.06E-4 ID:SGTC_1859|Compound:st055998|FD-Score:2.85|P-value:9.07E-4 ID:SGTC_1925|Compound:st057770|FD-Score:2.95|P-value:6.35E-4 ID:SGTC_1933|Compound:st074711|FD-Score:2.97|P-value:5.86E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.07|P-value:3.93E-4 ID:SGTC_2034|Compound:5197015|FD-Score:-2.93|P-value:8.39E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.51|P-value:6.40E-5 ID:SGTC_2126|Compound:5251174|FD-Score:4.98|P-value:2.92E-8 ID:SGTC_2137|Compound:5261020|FD-Score:4.31|P-value:1.30E-6 ID:SGTC_2226|Compound:7298446|FD-Score:3.03|P-value:4.60E-4 ID:SGTC_2278|Compound:7948257|FD-Score:4.74|P-value:1.19E-7 ID:SGTC_2348|Compound:9022543|FD-Score:10.00|P-value:4.70E-28 ID:SGTC_2446|Compound:5162541|FD-Score:-3.31|P-value:1.84E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.45|P-value:8.13E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.49|P-value:8.65E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.26|P-value:1.86E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:2.90|P-value:7.63E-4 ID:SGTC_2646|Compound:retinol|FD-Score:-3.04|P-value:5.51E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.03|P-value:4.51E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.29|P-value:1.45E-6 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.42|P-value:9.35E-5 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.07|P-value:4.54E-6 ID:SGTC_2873|Compound:9040596|FD-Score:2.93|P-value:6.83E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-5.04|P-value:2.81E-8 ID:SGTC_2973|Compound:9094665|FD-Score:3.00|P-value:5.06E-4 ID:SGTC_3132|Compound:9126706|FD-Score:3.34|P-value:1.30E-4 ID:SGTC_3208|Compound:9114115|FD-Score:2.91|P-value:7.29E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.01|P-value:6.00E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.28|P-value:1.95E-6 ID:SGTC_85|Compound:0868-0259|FD-Score:2.88|P-value:8.10E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:3.66|P-value:3.24E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:-3.01|P-value:6.19E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.80|P-value:1.71E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.22|P-value:2.16E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.13|P-value:3.87E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.87|P-value:8.36E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:3.69|P-value:2.80E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:3.44|P-value:8.38E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:7.24|P-value:1.66E-15 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.08|P-value:4.70E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.92|P-value:9.19E-6 ID:SGTC_535|Compound:2293-4129|FD-Score:3.83|P-value:1.44E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:3.28|P-value:1.69E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.94|P-value:8.48E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:2.91|P-value:7.19E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-7.86|P-value:1.04E-17 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.43|P-value:1.11E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.07|P-value:4.77E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.27|P-value:1.79E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.29|P-value:1.58E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:5.46|P-value:1.32E-9 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.05|P-value:4.32E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-5.94|P-value:7.35E-11 ID:SGTC_1493|Compound:4348-0038|FD-Score:5.05|P-value:1.90E-8 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:-3.65|P-value:4.20E-5 ID:SGTC_1673|Compound:st016611|FD-Score:5.11|P-value:1.27E-8 ID:SGTC_1712|Compound:st032288|FD-Score:3.80|P-value:1.67E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.54|P-value:5.49E-5 ID:SGTC_1804|Compound:st050076|FD-Score:-3.00|P-value:6.45E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.06|P-value:4.06E-4 ID:SGTC_1859|Compound:st055998|FD-Score:2.85|P-value:9.07E-4 ID:SGTC_1925|Compound:st057770|FD-Score:2.95|P-value:6.35E-4 ID:SGTC_1933|Compound:st074711|FD-Score:2.97|P-value:5.86E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.07|P-value:3.93E-4 ID:SGTC_2034|Compound:5197015|FD-Score:-2.93|P-value:8.39E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.51|P-value:6.40E-5 ID:SGTC_2126|Compound:5251174|FD-Score:4.98|P-value:2.92E-8 ID:SGTC_2137|Compound:5261020|FD-Score:4.31|P-value:1.30E-6 ID:SGTC_2226|Compound:7298446|FD-Score:3.03|P-value:4.60E-4 ID:SGTC_2278|Compound:7948257|FD-Score:4.74|P-value:1.19E-7 ID:SGTC_2348|Compound:9022543|FD-Score:10.00|P-value:4.70E-28 ID:SGTC_2446|Compound:5162541|FD-Score:-3.31|P-value:1.84E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.45|P-value:8.13E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.49|P-value:8.65E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.26|P-value:1.86E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:2.90|P-value:7.63E-4 ID:SGTC_2646|Compound:retinol|FD-Score:-3.04|P-value:5.51E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.03|P-value:4.51E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.29|P-value:1.45E-6 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.42|P-value:9.35E-5 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.07|P-value:4.54E-6 ID:SGTC_2873|Compound:9040596|FD-Score:2.93|P-value:6.83E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-5.04|P-value:2.81E-8 ID:SGTC_2973|Compound:9094665|FD-Score:3.00|P-value:5.06E-4 ID:SGTC_3132|Compound:9126706|FD-Score:3.34|P-value:1.30E-4 ID:SGTC_3208|Compound:9114115|FD-Score:2.91|P-value:7.29E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.01|P-value:6.00E-4

Top fitness defect scores for YGR005C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2348 9022543 10.00 33.4 µM 4.70E-28 mitochondrial processes 7 4.88E-24 0.59
2 SGTC_515 4092-1064 7.24 102.0 µM 1.66E-15 plasma membrane duress 5 2.25E-13 0.14
3 SGTC_1141 3253-0389 5.46 52.6 µM 1.32E-9 2 2.33E-8 1.43
4 SGTC_1673 st016611 5.11 27.3 µM 1.27E-8 1 1.60E-7 0.38
5 SGTC_1493 4348-0038 5.05 111.0 µM 1.90E-8 RSC complex & mRNA processing 26 2.26E-7 0.31
6 SGTC_2126 5251174 4.98 33.9 µM 2.92E-8 5 3.25E-7 0.75
7 SGTC_2278 7948257 4.74 134.3 µM 1.19E-7 2 1.07E-6 1.02
8 SGTC_2137 5261020 4.31 140.0 µM 1.30E-6 6 8.25E-6 0.38
9 SGTC_2764 methyldopa 4.29 94.7 µM 1.45E-6 2 9.05E-6 0.34
10 SGTC_2772 vinpocetine 4.07 57.1 µM 4.54E-6 6 2.39E-5 0.40
11 SGTC_673 1082-0474 3.94 110.0 µM 8.48E-6 RSC complex & mRNA processing 53 4.06E-5 0.01
12 SGTC_519 k048-0011 3.92 62.8 µM 9.19E-6 8 4.35E-5 0.12
13 SGTC_535 2293-4129 3.83 48.2 µM 1.44E-5 NEO1 10 6.37E-5 0.03
14 SGTC_1712 st032288 3.80 53.4 µM 1.67E-5 calcium & mitochondrial duress 38 7.22E-5 0.05
15 SGTC_218 alverine citrate 3.80 93.8 µM 1.71E-5 fatty acid desaturase (OLE1) 10 7.38E-5 0.03
16 SGTC_327 1273-0057 3.69 4.0 µM 2.80E-5 azole & statin 9 1.13E-4 0.11
17 SGTC_197 4544-0043 3.66 41.6 µM 3.24E-5 endomembrane recycling 26 1.27E-4 0.03
18 SGTC_1748 st037455 3.54 53.6 µM 5.49E-5 10 2.00E-4 0.00
19 SGTC_2041 5107442 3.51 45.3 µM 6.40E-5 RSC complex & mRNA processing 41 2.27E-4 0.04
20 SGTC_2455 5377712 3.45 9.8 µM 8.13E-5 9 2.79E-4 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.330 4.21E-86 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.117 1.01E-11 YOL019W-A_p YOL019W-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.115 2.28E-11 YMR043W MCM1 het Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
0.111 1.29E-10 YPL169C MEX67 het Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
0.107 4.24E-10 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.107 4.54E-10 YMR277W FCP1 het Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
0.105 1.07E-9 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.103 2.26E-9 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.098 1.29E-8 YOR042W CUE5 hom Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.097 1.98E-8 YKL084W HOT13 hom Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
0.095 3.92E-8 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.092 9.10E-8 YMR268C PRP24 het Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
0.092 1.06E-7 YGR115C_d YGR115C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
0.091 1.48E-7 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.090 2.04E-7 YDR357C_p CNL1_p hom Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm