YGR024C / THG1

tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR024C / THG1

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:-3.75|P-value:6.48E-5 ID:SGTC_51|Compound:1013-0126|FD-Score:4.13|P-value:1.48E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:3.43|P-value:2.58E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:3.28|P-value:4.55E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.25|P-value:5.17E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.06|P-value:9.92E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:3.66|P-value:1.08E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:-4.44|P-value:3.09E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.33|P-value:3.45E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:-3.29|P-value:3.91E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.19|P-value:6.26E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.06|P-value:2.02E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.05|P-value:9.42E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:3.89|P-value:4.19E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.39|P-value:3.09E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.13|P-value:7.72E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.58|P-value:1.45E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.24|P-value:4.72E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.20|P-value:5.44E-4 ID:SGTC_1606|Compound:st000094|FD-Score:-3.28|P-value:4.03E-4 ID:SGTC_1655|Compound:st012546|FD-Score:3.49|P-value:2.05E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.43|P-value:2.58E-4 ID:SGTC_2271|Compound:7849410|FD-Score:3.08|P-value:9.36E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.69|P-value:9.41E-5 ID:SGTC_2391|Compound:6285546|FD-Score:3.44|P-value:2.56E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.73|P-value:8.15E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-3.47|P-value:2.01E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.43|P-value:2.61E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.11|P-value:8.29E-4 ID:SGTC_2780|Compound:5215298|FD-Score:3.48|P-value:2.19E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.22|P-value:5.07E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.18|P-value:1.18E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.36|P-value:3.43E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.99|P-value:2.76E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.56|P-value:1.61E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.75|P-value:6.48E-5 ID:SGTC_51|Compound:1013-0126|FD-Score:4.13|P-value:1.48E-5 ID:SGTC_114|Compound:0364-0001|FD-Score:3.43|P-value:2.58E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:3.28|P-value:4.55E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.25|P-value:5.17E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.06|P-value:9.92E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:3.66|P-value:1.08E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:-4.44|P-value:3.09E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.33|P-value:3.45E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:-3.29|P-value:3.91E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.19|P-value:6.26E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.06|P-value:2.02E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.05|P-value:9.42E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:3.89|P-value:4.19E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.39|P-value:3.09E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.13|P-value:7.72E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.58|P-value:1.45E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.24|P-value:4.72E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.20|P-value:5.44E-4 ID:SGTC_1606|Compound:st000094|FD-Score:-3.28|P-value:4.03E-4 ID:SGTC_1655|Compound:st012546|FD-Score:3.49|P-value:2.05E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.43|P-value:2.58E-4 ID:SGTC_2271|Compound:7849410|FD-Score:3.08|P-value:9.36E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.69|P-value:9.41E-5 ID:SGTC_2391|Compound:6285546|FD-Score:3.44|P-value:2.56E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.73|P-value:8.15E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-3.47|P-value:2.01E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.43|P-value:2.61E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.11|P-value:8.29E-4 ID:SGTC_2780|Compound:5215298|FD-Score:3.48|P-value:2.19E-4 ID:SGTC_2792|Compound:7535235|FD-Score:-3.22|P-value:5.07E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.18|P-value:1.18E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.36|P-value:3.43E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.99|P-value:2.76E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.56|P-value:1.61E-4

Top fitness defect scores for YGR024C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2881 9058953 4.18 52.0 µM 1.18E-5 3 1.47E-5 0.02
2 SGTC_51 1013-0126 4.13 23.4 µM 1.48E-5 5 1.83E-5 0.35
3 SGTC_1149 3909-7961 4.06 23.3 µM 2.02E-5 5 2.48E-5 0.37
4 SGTC_2906 7926292 3.99 26.4 µM 2.76E-5 4 3.36E-5 0.05
5 SGTC_1269 0831-0796 3.89 3.0 µM 4.19E-5 60S ribosome export 7 5.04E-5 0.15
6 SGTC_2394 5221648 3.73 200.0 µM 8.15E-5 7 9.65E-5 0.03
7 SGTC_2272 7805186 3.69 180.0 µM 9.41E-5 cell wall signaling 4 1.11E-4 0.10
8 SGTC_806 3966-4455 3.66 5.9 µM 1.08E-4 10 1.27E-4 0.03
9 SGTC_1335 1486-0900 3.58 202.0 µM 1.45E-4 5 1.70E-4 0.00
10 SGTC_2975 9084847 3.56 53.7 µM 1.61E-4 12 1.87E-4 0.09
11 SGTC_1655 st012546 3.49 55.1 µM 2.05E-4 9 2.37E-4 0.34
12 SGTC_2780 5215298 3.48 71.4 µM 2.19E-4 18 2.53E-4 0.02
13 SGTC_2391 6285546 3.44 200.0 µM 2.56E-4 15 2.95E-4 0.06
14 SGTC_114 0364-0001 3.43 490.3 µM 2.58E-4 11 2.96E-4 0.03
15 SGTC_1679 4130-1276 3.43 6.7 µM 2.58E-4 Golgi 33 2.97E-4 0.06
16 SGTC_2552 juarezic acid 3.43 87.0 µM 2.61E-4 TSC3-RPN4 6 3.00E-4 0.43
17 SGTC_1287 0971-0001 3.39 34.6 µM 3.09E-4 sphingolipid biosynthesis & PDR1 7 3.54E-4 0.13
18 SGTC_2882 9058044 3.36 58.4 µM 3.43E-4 13 3.92E-4 0.03
19 SGTC_123 0083-0120 3.28 66.7 µM 4.55E-4 19 5.16E-4 0.01
20 SGTC_188 3229-0966 3.25 278.5 µM 5.17E-4 30 5.85E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.164 8.95E-22 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.132 2.06E-14 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.120 2.75E-12 YKR080W MTD1 hom NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
0.103 2.52E-9 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.095 3.11E-8 YOR315W SFG1 hom Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate
0.090 2.06E-7 YJR025C BNA1 hom 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
0.085 7.23E-7 YDL231C BRE4 hom Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
0.084 1.26E-6 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.083 1.30E-6 YGL229C SAP4 hom Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p
0.083 1.68E-6 YOR245C DGA1 hom Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
0.081 2.40E-6 YGL065C ALG2 het Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
0.081 2.87E-6 YGL062W PYC1 hom Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
0.079 4.56E-6 YPL260W YPL260W hom Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
0.078 5.34E-6 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.078 6.94E-6 YGR066C_p YGR066C_p hom Putative protein of unknown function