YGR027C / RPS25A

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR027C / RPS25A

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.79|P-value:3.20E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.44|P-value:9.74E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:7.05|P-value:1.12E-10 ID:SGTC_145|Compound:3737-0113|FD-Score:4.51|P-value:2.42E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.60|P-value:5.98E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.69|P-value:4.27E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:4.76|P-value:9.28E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:4.85|P-value:6.35E-6 ID:SGTC_993|Compound:3909-8757|FD-Score:3.75|P-value:3.76E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:4.49|P-value:2.63E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:6.23|P-value:1.03E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.15|P-value:9.29E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:3.76|P-value:3.54E-4 ID:SGTC_1740|Compound:st038286|FD-Score:4.08|P-value:1.19E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.60|P-value:6.08E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.18|P-value:1.58E-6 ID:SGTC_2184|Compound:6075246|FD-Score:-4.50|P-value:2.48E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.47|P-value:8.99E-4 ID:SGTC_2427|Compound:5545055|FD-Score:4.87|P-value:5.90E-6 ID:SGTC_2436|Compound:5633522|FD-Score:4.66|P-value:1.39E-5 ID:SGTC_2549|Compound:cedrol|FD-Score:4.10|P-value:1.14E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-4.94|P-value:4.09E-6 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.58|P-value:6.49E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.66|P-value:1.39E-5 ID:SGTC_2692|Compound:st077426|FD-Score:-3.58|P-value:6.24E-4 ID:SGTC_3285|Compound:9112818|FD-Score:5.59|P-value:2.41E-7 ID:SGTC_3312|Compound:9133330|FD-Score:3.53|P-value:7.51E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.79|P-value:3.20E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.44|P-value:9.74E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:7.05|P-value:1.12E-10 ID:SGTC_145|Compound:3737-0113|FD-Score:4.51|P-value:2.42E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.60|P-value:5.98E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:-3.69|P-value:4.27E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:4.76|P-value:9.28E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:4.85|P-value:6.35E-6 ID:SGTC_993|Compound:3909-8757|FD-Score:3.75|P-value:3.76E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:4.49|P-value:2.63E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:6.23|P-value:1.03E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.15|P-value:9.29E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:3.76|P-value:3.54E-4 ID:SGTC_1740|Compound:st038286|FD-Score:4.08|P-value:1.19E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.60|P-value:6.08E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.18|P-value:1.58E-6 ID:SGTC_2184|Compound:6075246|FD-Score:-4.50|P-value:2.48E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.47|P-value:8.99E-4 ID:SGTC_2427|Compound:5545055|FD-Score:4.87|P-value:5.90E-6 ID:SGTC_2436|Compound:5633522|FD-Score:4.66|P-value:1.39E-5 ID:SGTC_2549|Compound:cedrol|FD-Score:4.10|P-value:1.14E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-4.94|P-value:4.09E-6 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.58|P-value:6.49E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.66|P-value:1.39E-5 ID:SGTC_2692|Compound:st077426|FD-Score:-3.58|P-value:6.24E-4 ID:SGTC_3285|Compound:9112818|FD-Score:5.59|P-value:2.41E-7 ID:SGTC_3312|Compound:9133330|FD-Score:3.53|P-value:7.51E-4

Top fitness defect scores for YGR027C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_103 0844-0104 7.05 51.8 µM 1.12E-10 ERAD & cell cycle 60 9.25E-13
2 SGTC_1082 camptothecin 6.23 424.9 nM 1.03E-8 DNA damage response 18 2.38E-10
3 SGTC_3285 9112818 5.59 20.9 µM 2.41E-7 cell wall 10 1.12E-8
4 SGTC_1844 rapamycin 5.18 4.0 nM 1.58E-6 110 1.13E-7
5 SGTC_2427 5545055 4.87 42.8 µM 5.90E-6 5 5.64E-7
6 SGTC_733 4069-0007 4.85 56.1 µM 6.35E-6 ERAD & cell cycle 56 6.17E-7
7 SGTC_660 4031-1742 4.76 102.0 µM 9.28E-6 redox potentiating 26 9.81E-7
8 SGTC_2679 rapamycin 4.66 1.0 nM 1.39E-5 107 1.61E-6
9 SGTC_2436 5633522 4.66 8.1 µM 1.39E-5 8 1.61E-6
10 SGTC_145 3737-0113 4.51 38.4 µM 2.42E-5 DNA intercalators 56 3.17E-6
11 SGTC_998 3996-0085 4.49 177.0 µM 2.63E-5 11 3.51E-6
12 SGTC_1130 3454-2858 4.15 15.9 µM 9.29E-5 NEO1 78 1.64E-5
13 SGTC_2549 cedrol 4.10 20.9 µM 1.14E-4 15 2.10E-5
14 SGTC_1740 st038286 4.08 13.2 µM 1.19E-4 24 2.22E-5
15 SGTC_37 0566-0070 3.79 1.6 µM 3.20E-4 31 7.39E-5
16 SGTC_1275 0867-0008 3.76 21.1 µM 3.54E-4 16 8.36E-5
17 SGTC_993 3909-8757 3.75 62.7 µM 3.76E-4 30 9.00E-5
18 SGTC_415 dibenzyl ketone 3.60 100.0 µM 5.98E-4 17 1.58E-4
19 SGTC_1786 berberine 3.60 21.6 µM 6.08E-4 60S ribosome export 44 1.62E-4
20 SGTC_2618 avocadyne 3.58 43.4 µM 6.49E-4 39 1.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.194 9.41E-30 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.187 9.57E-28 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.184 5.00E-27 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.161 6.98E-21 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.161 7.99E-21 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.160 9.43E-21 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.147 9.98E-18 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.146 1.57E-17 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.145 3.46E-17 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.142 1.11E-16 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.138 9.32E-16 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.137 1.33E-15 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.136 2.32E-15 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.132 1.68E-14 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.132 1.71E-14 YKR095W MLP1 hom Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs