YGR048W / UFD1

Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR048W / UFD1

Click on Significant Values for Screen Details ID:SGTC_34|Compound:0469-0683|FD-Score:3.48|P-value:9.37E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.55|P-value:6.91E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:3.66|P-value:5.34E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:-3.50|P-value:8.23E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:5.98|P-value:4.26E-8 ID:SGTC_996|Compound:3970-0799|FD-Score:9.19|P-value:9.12E-17 ID:SGTC_1125|Compound:tnp00248|FD-Score:5.56|P-value:3.25E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.07|P-value:1.36E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.12|P-value:1.15E-4 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.94|P-value:2.14E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.68|P-value:5.03E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.57|P-value:7.11E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.94|P-value:2.15E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.66|P-value:5.22E-4 ID:SGTC_2281|Compound:7943571|FD-Score:5.65|P-value:2.06E-7 ID:SGTC_2793|Compound:7325131|FD-Score:5.51|P-value:4.12E-7 ID:SGTC_2797|Compound:7859098|FD-Score:-4.21|P-value:7.72E-5 ID:SGTC_2798|Compound:7813637|FD-Score:5.46|P-value:5.11E-7 ID:SGTC_2800|Compound:7887935|FD-Score:5.16|P-value:1.94E-6 ID:SGTC_2886|Compound:9058944|FD-Score:-3.48|P-value:8.76E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.65|P-value:5.50E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-4.12|P-value:1.06E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.92|P-value:2.26E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.87|P-value:2.71E-4 ID:SGTC_3151|Compound:9099821|FD-Score:4.08|P-value:1.29E-4 ID:SGTC_3222|Compound:9128301|FD-Score:3.74|P-value:4.04E-4 ID:SGTC_3338|Compound:9144300|FD-Score:-3.69|P-value:4.56E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.48|P-value:9.37E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.55|P-value:6.91E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:3.66|P-value:5.34E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:-3.50|P-value:8.23E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:5.98|P-value:4.26E-8 ID:SGTC_996|Compound:3970-0799|FD-Score:9.19|P-value:9.12E-17 ID:SGTC_1125|Compound:tnp00248|FD-Score:5.56|P-value:3.25E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.07|P-value:1.36E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.12|P-value:1.15E-4 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.94|P-value:2.14E-4 ID:SGTC_1870|Compound:st057514|FD-Score:3.68|P-value:5.03E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.57|P-value:7.11E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.94|P-value:2.15E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.66|P-value:5.22E-4 ID:SGTC_2281|Compound:7943571|FD-Score:5.65|P-value:2.06E-7 ID:SGTC_2793|Compound:7325131|FD-Score:5.51|P-value:4.12E-7 ID:SGTC_2797|Compound:7859098|FD-Score:-4.21|P-value:7.72E-5 ID:SGTC_2798|Compound:7813637|FD-Score:5.46|P-value:5.11E-7 ID:SGTC_2800|Compound:7887935|FD-Score:5.16|P-value:1.94E-6 ID:SGTC_2886|Compound:9058944|FD-Score:-3.48|P-value:8.76E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.65|P-value:5.50E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-4.12|P-value:1.06E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.92|P-value:2.26E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.87|P-value:2.71E-4 ID:SGTC_3151|Compound:9099821|FD-Score:4.08|P-value:1.29E-4 ID:SGTC_3222|Compound:9128301|FD-Score:3.74|P-value:4.04E-4 ID:SGTC_3338|Compound:9144300|FD-Score:-3.69|P-value:4.56E-4

Top fitness defect scores for YGR048W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_996 3970-0799 9.19 1.8 µM 9.12E-17 3 2.01E-20 0.08
2 SGTC_795 0685-0265 5.98 78.0 µM 4.26E-8 excess fatty acid 4 1.11E-9 0.66
3 SGTC_2281 7943571 5.65 200.0 µM 2.06E-7 1 7.81E-9 1.06
4 SGTC_1125 tnp00248 5.56 1.3 µM 3.25E-7 4 1.38E-8 0.36
5 SGTC_2793 7325131 5.51 81.8 µM 4.12E-7 2 1.84E-8 0.75
6 SGTC_2798 7813637 5.46 45.5 µM 5.11E-7 4 2.40E-8 0.08
7 SGTC_2800 7887935 5.16 81.8 µM 1.94E-6 RPP1 & pyrimidine depletion 4 1.25E-7 0.09
8 SGTC_1373 2887-0003 4.12 58.5 µM 1.15E-4 Golgi 10 1.93E-5 0.02
9 SGTC_3151 9099821 4.08 49.5 µM 1.29E-4 3 2.22E-5 0.23
10 SGTC_1204 4049-0315 4.07 18.3 µM 1.36E-4 10 2.36E-5 0.30
11 SGTC_1546 5-methoxyflavanone 3.94 78.7 µM 2.14E-4 2 4.12E-5 0.52
12 SGTC_1906 st060206 3.94 94.7 µM 2.15E-4 3 4.15E-5 0.39
13 SGTC_2989 9073649 3.92 71.4 µM 2.26E-4 7 4.40E-5 0.07
14 SGTC_3147 9097027 3.87 49.5 µM 2.71E-4 9 5.53E-5 0.02
15 SGTC_3222 9128301 3.74 49.5 µM 4.04E-4 60S ribosome export 8 9.03E-5 0.20
16 SGTC_1870 st057514 3.68 26.5 µM 5.03E-4 RSC & ERG11 12 1.18E-4 0.28
17 SGTC_1963 st076565 3.66 50.3 µM 5.22E-4 5 1.24E-4 0.02
18 SGTC_300 r073-0011 3.66 5.0 µM 5.34E-4 3 1.27E-4 0.21
19 SGTC_2947 9072639 3.65 7.7 µM 5.50E-4 tubulin folding & SWR complex 13 1.32E-4 0.12
20 SGTC_1892 st058411 3.57 7.4 µM 7.11E-4 8 1.81E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.305 4.50E-73 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.247 7.02E-48 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.239 1.07E-44 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.204 6.17E-33 YJL149W DAS1 hom Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
0.203 1.49E-32 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.203 1.32E-32 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.187 7.13E-28 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.185 2.44E-27 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.182 2.99E-26 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.161 7.14E-21 YCL044C MGR1 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.159 2.10E-20 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.154 3.40E-19 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.150 2.24E-18 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.147 1.12E-17 YGL207W SPT16 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
0.146 1.86E-17 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene