YGR054W

Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR054W / YGR054W

Click on Significant Values for Screen Details ID:SGTC_62|Compound:0180-0423|FD-Score:-3.94|P-value:3.72E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:3.84|P-value:5.20E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.39|P-value:3.30E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:3.07|P-value:9.44E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.89|P-value:4.69E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.05|P-value:2.39E-5 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.83|P-value:6.10E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:3.14|P-value:7.49E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.53|P-value:1.95E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.00|P-value:2.67E-5 ID:SGTC_283|Compound:0987-0079|FD-Score:3.06|P-value:9.76E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:7.47|P-value:2.31E-14 ID:SGTC_384|Compound:k007-0144|FD-Score:4.80|P-value:6.18E-7 ID:SGTC_444|Compound:curcumin|FD-Score:3.59|P-value:1.43E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:-3.18|P-value:7.04E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:6.89|P-value:1.75E-12 ID:SGTC_749|Compound:1000-0129|FD-Score:4.29|P-value:7.08E-6 ID:SGTC_755|Compound:1995-0008|FD-Score:-3.26|P-value:5.40E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.12|P-value:8.79E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.13|P-value:1.68E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.57|P-value:1.66E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.28|P-value:4.92E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.25|P-value:4.97E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.11|P-value:1.62E-5 ID:SGTC_1408|Compound:3909-7976|FD-Score:-3.14|P-value:8.26E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:4.34|P-value:5.76E-6 ID:SGTC_1516|Compound:st013856|FD-Score:-3.92|P-value:4.17E-5 ID:SGTC_1687|Compound:st020778|FD-Score:-3.11|P-value:9.16E-4 ID:SGTC_1688|Compound:st021091|FD-Score:4.75|P-value:7.99E-7 ID:SGTC_1719|Compound:st032227|FD-Score:-3.62|P-value:1.38E-4 ID:SGTC_1720|Compound:st037298|FD-Score:-3.59|P-value:1.55E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.63|P-value:1.35E-4 ID:SGTC_1935|Compound:st074532|FD-Score:-3.77|P-value:7.55E-5 ID:SGTC_1937|Compound:st074714|FD-Score:-3.29|P-value:4.86E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.20|P-value:6.71E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.78|P-value:6.72E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-4.61|P-value:1.84E-6 ID:SGTC_2570|Compound:licareol|FD-Score:-3.45|P-value:2.64E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.15|P-value:9.81E-8 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.34|P-value:3.63E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.32|P-value:4.35E-4 ID:SGTC_2879|Compound:9041815|FD-Score:3.20|P-value:6.05E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.48|P-value:2.18E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.27|P-value:5.12E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.41|P-value:2.85E-4 ID:SGTC_3228|Compound:9131799|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-3.56|P-value:1.73E-4 ID:SGTC_3241|Compound:9133421|FD-Score:-3.45|P-value:2.65E-4 ID:SGTC_3243|Compound:9134147|FD-Score:-3.86|P-value:5.21E-5 ID:SGTC_3245|Compound:9134821|FD-Score:-3.99|P-value:3.09E-5 ID:SGTC_3252|Compound:9135871|FD-Score:5.44|P-value:1.92E-8 ID:SGTC_3297|Compound:9117502|FD-Score:3.21|P-value:5.76E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.93|P-value:3.48E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.94|P-value:3.72E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:3.84|P-value:5.20E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.39|P-value:3.30E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:3.07|P-value:9.44E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.89|P-value:4.69E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.05|P-value:2.39E-5 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.83|P-value:6.10E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:3.14|P-value:7.49E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.53|P-value:1.95E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.00|P-value:2.67E-5 ID:SGTC_283|Compound:0987-0079|FD-Score:3.06|P-value:9.76E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:7.47|P-value:2.31E-14 ID:SGTC_384|Compound:k007-0144|FD-Score:4.80|P-value:6.18E-7 ID:SGTC_444|Compound:curcumin|FD-Score:3.59|P-value:1.43E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:-3.18|P-value:7.04E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:6.89|P-value:1.75E-12 ID:SGTC_749|Compound:1000-0129|FD-Score:4.29|P-value:7.08E-6 ID:SGTC_755|Compound:1995-0008|FD-Score:-3.26|P-value:5.40E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.12|P-value:8.79E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.13|P-value:1.68E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.57|P-value:1.66E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.28|P-value:4.92E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.25|P-value:4.97E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.11|P-value:1.62E-5 ID:SGTC_1408|Compound:3909-7976|FD-Score:-3.14|P-value:8.26E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:4.34|P-value:5.76E-6 ID:SGTC_1516|Compound:st013856|FD-Score:-3.92|P-value:4.17E-5 ID:SGTC_1687|Compound:st020778|FD-Score:-3.11|P-value:9.16E-4 ID:SGTC_1688|Compound:st021091|FD-Score:4.75|P-value:7.99E-7 ID:SGTC_1719|Compound:st032227|FD-Score:-3.62|P-value:1.38E-4 ID:SGTC_1720|Compound:st037298|FD-Score:-3.59|P-value:1.55E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.63|P-value:1.35E-4 ID:SGTC_1935|Compound:st074532|FD-Score:-3.77|P-value:7.55E-5 ID:SGTC_1937|Compound:st074714|FD-Score:-3.29|P-value:4.86E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.20|P-value:6.71E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.78|P-value:6.72E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-4.61|P-value:1.84E-6 ID:SGTC_2570|Compound:licareol|FD-Score:-3.45|P-value:2.64E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.15|P-value:9.81E-8 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.34|P-value:3.63E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.32|P-value:4.35E-4 ID:SGTC_2879|Compound:9041815|FD-Score:3.20|P-value:6.05E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.48|P-value:2.18E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.27|P-value:5.12E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.41|P-value:2.85E-4 ID:SGTC_3228|Compound:9131799|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-3.56|P-value:1.73E-4 ID:SGTC_3241|Compound:9133421|FD-Score:-3.45|P-value:2.65E-4 ID:SGTC_3243|Compound:9134147|FD-Score:-3.86|P-value:5.21E-5 ID:SGTC_3245|Compound:9134821|FD-Score:-3.99|P-value:3.09E-5 ID:SGTC_3252|Compound:9135871|FD-Score:5.44|P-value:1.92E-8 ID:SGTC_3297|Compound:9117502|FD-Score:3.21|P-value:5.76E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.93|P-value:3.48E-5

Top fitness defect scores for YGR054W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_373 0844-0104 7.47 25.9 µM 2.31E-14 ERAD & cell cycle 15 3.97E-14
2 SGTC_733 4069-0007 6.89 56.1 µM 1.75E-12 ERAD & cell cycle 29 2.80E-12
3 SGTC_3252 9135871 5.44 49.5 µM 1.92E-8 2 2.62E-8
4 SGTC_2651 1327-0036 5.15 5.0 µM 9.81E-8 104 1.30E-7
5 SGTC_384 k007-0144 4.80 5.2 µM 6.18E-7 ERAD & cell cycle 38 7.96E-7
6 SGTC_1688 st021091 4.75 57.6 µM 7.99E-7 1 1.02E-6
7 SGTC_1453 4237-0541 4.34 195.0 µM 5.76E-6 heme biosynthesis & mitochondrial translocase 19 7.14E-6
8 SGTC_749 1000-0129 4.29 128.0 µM 7.08E-6 amide catabolism 12 8.74E-6
9 SGTC_1230 0170-0365 4.11 3.8 µM 1.62E-5 endomembrane recycling 60 1.97E-5
10 SGTC_163 1327-0036 4.00 7.5 µM 2.67E-5 101 3.22E-5
11 SGTC_3346 9158254 3.93 43.6 µM 3.48E-5 14 4.17E-5
12 SGTC_64 000l-0018 3.84 272.1 µM 5.20E-5 ubiquinone biosynthesis & proteasome 155 6.20E-5
13 SGTC_2322 7413319 3.78 38.2 µM 6.72E-5 ERAD & cell cycle 164 7.98E-5
14 SGTC_3228 9131799 3.63 49.5 µM 1.19E-4 amide catabolism 10 1.40E-4
15 SGTC_444 curcumin 3.59 90.4 µM 1.43E-4 iron homeostasis 37 1.67E-4
16 SGTC_3033 9090887 3.48 49.5 µM 2.18E-4 fatty acid desaturase (OLE1) 44 2.53E-4
17 SGTC_3059 1327-0034 3.41 3.0 µM 2.85E-4 74 3.30E-4
18 SGTC_2765 myclobutanil 3.34 7.7 µM 3.63E-4 azole & statin 73 4.18E-4
19 SGTC_1035 k081-0041 3.25 55.2 µM 4.97E-4 Golgi 46 5.69E-4
20 SGTC_3297 9117502 3.21 71.4 µM 5.76E-4 27 6.58E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.308 1.04E-74 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.231 4.83E-42 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.191 8.31E-29 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.188 3.73E-28 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.182 1.59E-26 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.177 5.06E-25 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.175 2.11E-24 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.158 3.09E-20 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.156 1.08E-19 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.151 1.73E-18 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.150 1.87E-18 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.144 4.92E-17 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.140 4.46E-16 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.134 5.79E-15 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
0.130 4.85E-14 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress