HIPHOP chemogenomics database

YGR060W / ERG25

C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol

Zygosity: Heterozygous strain

fixedexpanded
Profile for YGR060W / ERG25

Click on Significant Values for Screen Details

Top fitness defect scores for YGR060W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_637	1181-0519	21.00	7.3 µM	2.89E-72	fatty acid desaturase (OLE1)	1	8.32E-98	8.69
2	SGTC_568	1193-0097	17.50	7.5 µM	4.14E-51	ERAD & cell cycle	2	5.42E-69	3.47
3	SGTC_768	0384-0033	13.20	77.3 µM	6.40E-30	fatty acid desaturase (OLE1)	2	3.84E-40	2.84
4	SGTC_374	0880-0231	11.60	82.3 µM	1.60E-23	iron homeostasis	1	1.95E-31	4.68
5	SGTC_834	0312-0008	10.10	75.7 µM	2.19E-18	NEO1	3	1.90E-24	0.38
6	SGTC_1754	st042065	10.00	16.1 µM	4.99E-18	NEO1-PIK1	5	5.81E-24	0.69
7	SGTC_352	k048-0007	9.31	5.1 µM	8.47E-16	ERAD & cell cycle	1	6.26E-21	2.89
8	SGTC_649	1123-0311	8.86	367.0 µM	1.85E-14	endomembrane recycling	3	4.14E-19	1.13
9	SGTC_2257	7960413	8.34	200.0 µM	5.09E-13	NEO1-PIK1	4	3.74E-17	0.34
10	SGTC_206	4546-0025	8.08	72.0 µM	2.54E-12	NEO1	3	3.32E-16	2.17
11	SGTC_1230	0170-0365	7.90	3.8 µM	7.25E-12	endomembrane recycling	8	1.38E-15	0.83
12	SGTC_207	4185-0010	7.18	4.5 µM	4.36E-10	endomembrane recycling	5	3.59E-13	0.55
13	SGTC_384	k007-0144	7.15	5.2 µM	5.13E-10	ERAD & cell cycle	1	4.48E-13	1.26
14	SGTC_875	0709-0210	6.22	25.7 µM	5.36E-8	fatty acid desaturase (OLE1)	4	2.45E-10	0.48
15	SGTC_23	0213-0011	5.67	40.0 µM	6.34E-7	copper-dependent oxidative stress	1	6.95E-9	1.05
16	SGTC_1712	st032288	5.49	53.4 µM	1.38E-6	calcium & mitochondrial duress	17	1.99E-8	0.02
17	SGTC_15	0266-0086	5.22	27.1 µM	4.20E-6	endomembrane recycling	8	8.98E-8	0.94
18	SGTC_2006	4011487	5.14	200.0 µM	5.82E-6	endomembrane recycling	11	1.39E-7	0.39
19	SGTC_1308	1187-1617	4.96	41.4 µM	1.18E-5	fatty acid desaturase (OLE1)	3	3.60E-7	0.25
20	SGTC_515	4092-1064	4.66	102.0 µM	3.46E-5	plasma membrane duress	12	1.55E-6	0.12

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.444	3.89E-162	YMR134W	ERG29	het	Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.376	3.82E-113	YDL133W	SRF1	hom	Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.265	6.71E-55	YML037C_p	YML037C_p	hom	Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.264	1.93E-54	YLR057W	MNL2	hom	Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.244	9.73E-47	YDR456W	NHX1	hom	Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.242	6.76E-46	YPL120W	VPS30	hom	Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.228	7.08E-41	YOR104W	PIN2	hom	Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated
0.225	1.18E-39	YDR372C	VPS74	hom	Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.220	7.25E-38	YOL164W	BDS1	hom	Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources
0.219	8.57E-38	YOL144W	NOP8	het	Nucleolar protein required for 60S ribosomal subunit biogenesis
0.218	2.19E-37	YKL039W	PTM1	hom	Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
0.214	4.87E-36	YBL022C	PIM1	hom	ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.210	7.66E-35	YLR380W	CSR1	hom	Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.209	1.65E-34	YIL030C	SSM4	hom	Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.206	1.95E-33	YNL267W	PIK1	het	Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton