YGR063C / SPT4

Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR063C / SPT4

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:4.28|P-value:2.25E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:6.31|P-value:9.59E-8 ID:SGTC_100|Compound:0086-0128|FD-Score:4.55|P-value:9.53E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.65|P-value:6.73E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:7.06|P-value:2.75E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:5.18|P-value:1.01E-5 ID:SGTC_164|Compound:4100-3994|FD-Score:3.87|P-value:7.83E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:4.36|P-value:1.78E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.37|P-value:5.05E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.74|P-value:7.13E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.32|P-value:5.91E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.46|P-value:7.33E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.44|P-value:1.36E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:6.98|P-value:4.12E-9 ID:SGTC_562|Compound:3772-2733|FD-Score:5.09|P-value:1.41E-5 ID:SGTC_571|Compound:k831-0106|FD-Score:3.86|P-value:8.03E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:4.06|P-value:4.42E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:4.41|P-value:1.52E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:4.22|P-value:2.70E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:4.82|P-value:3.77E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.81|P-value:5.81E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:5.72|P-value:1.22E-6 ID:SGTC_1230|Compound:0170-0365|FD-Score:-4.51|P-value:6.20E-5 ID:SGTC_1304|Compound:1165-0502|FD-Score:13.00|P-value:2.04E-27 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.19|P-value:2.98E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.11|P-value:3.81E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:6.41|P-value:6.24E-8 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.97|P-value:5.85E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.90|P-value:7.03E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:3.89|P-value:7.33E-4 ID:SGTC_1630|Compound:st005209|FD-Score:4.99|P-value:2.03E-5 ID:SGTC_1632|Compound:st005650|FD-Score:3.86|P-value:8.06E-4 ID:SGTC_1687|Compound:st020778|FD-Score:5.46|P-value:3.48E-6 ID:SGTC_1801|Compound:st050089|FD-Score:5.24|P-value:8.05E-6 ID:SGTC_1974|Compound:st069448|FD-Score:3.97|P-value:5.76E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-5.06|P-value:8.76E-6 ID:SGTC_2083|Compound:5306046|FD-Score:4.23|P-value:2.64E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:-4.48|P-value:7.04E-5 ID:SGTC_2289|Compound:7946404|FD-Score:6.10|P-value:2.52E-7 ID:SGTC_2308|Compound:7584454|FD-Score:-4.57|P-value:5.16E-5 ID:SGTC_2315|Compound:7733312|FD-Score:-3.91|P-value:4.29E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.90|P-value:7.14E-4 ID:SGTC_2421|Compound:5690543|FD-Score:-3.71|P-value:7.74E-4 ID:SGTC_2442|Compound:5158906|FD-Score:4.03|P-value:4.89E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-4.78|P-value:2.40E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.57|P-value:2.30E-6 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:6.69|P-value:1.68E-8 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-3.64|P-value:9.43E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.95|P-value:3.83E-4 ID:SGTC_2715|Compound:st079500|FD-Score:4.35|P-value:1.81E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.00|P-value:5.38E-4 ID:SGTC_2838|Compound:9002687|FD-Score:-8.82|P-value:5.28E-14 ID:SGTC_2843|Compound:9000894|FD-Score:4.38|P-value:1.64E-4 ID:SGTC_2845|Compound:9003024|FD-Score:17.90|P-value:2.26E-50 ID:SGTC_2900|Compound:5328528|FD-Score:3.93|P-value:6.53E-4 ID:SGTC_2932|Compound:9016711|FD-Score:4.83|P-value:3.65E-5 ID:SGTC_2976|Compound:9009005|FD-Score:9.67|P-value:5.64E-16 ID:SGTC_2978|Compound:9011784|FD-Score:7.22|P-value:1.22E-9 ID:SGTC_2980|Compound:9014342|FD-Score:12.20|P-value:3.29E-24 ID:SGTC_2997|Compound:9067075|FD-Score:4.93|P-value:2.58E-5 ID:SGTC_3038|Compound:9091290|FD-Score:3.95|P-value:6.20E-4 ID:SGTC_3098|Compound:9119039|FD-Score:5.46|P-value:3.54E-6 ID:SGTC_3200|Compound:9114385|FD-Score:5.10|P-value:1.39E-5 ID:SGTC_3237|Compound:9133362|FD-Score:4.16|P-value:3.26E-4 ID:SGTC_3339|Compound:9145883|FD-Score:-3.80|P-value:5.91E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:4.28|P-value:2.25E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:6.31|P-value:9.59E-8 ID:SGTC_100|Compound:0086-0128|FD-Score:4.55|P-value:9.53E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.65|P-value:6.73E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:7.06|P-value:2.75E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:5.18|P-value:1.01E-5 ID:SGTC_164|Compound:4100-3994|FD-Score:3.87|P-value:7.83E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:4.36|P-value:1.78E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.37|P-value:5.05E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.74|P-value:7.13E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.32|P-value:5.91E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-4.46|P-value:7.33E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.44|P-value:1.36E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:6.98|P-value:4.12E-9 ID:SGTC_562|Compound:3772-2733|FD-Score:5.09|P-value:1.41E-5 ID:SGTC_571|Compound:k831-0106|FD-Score:3.86|P-value:8.03E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:4.06|P-value:4.42E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:4.41|P-value:1.52E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:4.22|P-value:2.70E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:4.82|P-value:3.77E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.81|P-value:5.81E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:5.72|P-value:1.22E-6 ID:SGTC_1230|Compound:0170-0365|FD-Score:-4.51|P-value:6.20E-5 ID:SGTC_1304|Compound:1165-0502|FD-Score:13.00|P-value:2.04E-27 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.19|P-value:2.98E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.11|P-value:3.81E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:6.41|P-value:6.24E-8 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.97|P-value:5.85E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.90|P-value:7.03E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:3.89|P-value:7.33E-4 ID:SGTC_1630|Compound:st005209|FD-Score:4.99|P-value:2.03E-5 ID:SGTC_1632|Compound:st005650|FD-Score:3.86|P-value:8.06E-4 ID:SGTC_1687|Compound:st020778|FD-Score:5.46|P-value:3.48E-6 ID:SGTC_1801|Compound:st050089|FD-Score:5.24|P-value:8.05E-6 ID:SGTC_1974|Compound:st069448|FD-Score:3.97|P-value:5.76E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-5.06|P-value:8.76E-6 ID:SGTC_2083|Compound:5306046|FD-Score:4.23|P-value:2.64E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:-4.48|P-value:7.04E-5 ID:SGTC_2289|Compound:7946404|FD-Score:6.10|P-value:2.52E-7 ID:SGTC_2308|Compound:7584454|FD-Score:-4.57|P-value:5.16E-5 ID:SGTC_2315|Compound:7733312|FD-Score:-3.91|P-value:4.29E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.90|P-value:7.14E-4 ID:SGTC_2421|Compound:5690543|FD-Score:-3.71|P-value:7.74E-4 ID:SGTC_2442|Compound:5158906|FD-Score:4.03|P-value:4.89E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-4.78|P-value:2.40E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.57|P-value:2.30E-6 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:6.69|P-value:1.68E-8 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-3.64|P-value:9.43E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.95|P-value:3.83E-4 ID:SGTC_2715|Compound:st079500|FD-Score:4.35|P-value:1.81E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.00|P-value:5.38E-4 ID:SGTC_2838|Compound:9002687|FD-Score:-8.82|P-value:5.28E-14 ID:SGTC_2843|Compound:9000894|FD-Score:4.38|P-value:1.64E-4 ID:SGTC_2845|Compound:9003024|FD-Score:17.90|P-value:2.26E-50 ID:SGTC_2900|Compound:5328528|FD-Score:3.93|P-value:6.53E-4 ID:SGTC_2932|Compound:9016711|FD-Score:4.83|P-value:3.65E-5 ID:SGTC_2976|Compound:9009005|FD-Score:9.67|P-value:5.64E-16 ID:SGTC_2978|Compound:9011784|FD-Score:7.22|P-value:1.22E-9 ID:SGTC_2980|Compound:9014342|FD-Score:12.20|P-value:3.29E-24 ID:SGTC_2997|Compound:9067075|FD-Score:4.93|P-value:2.58E-5 ID:SGTC_3038|Compound:9091290|FD-Score:3.95|P-value:6.20E-4 ID:SGTC_3098|Compound:9119039|FD-Score:5.46|P-value:3.54E-6 ID:SGTC_3200|Compound:9114385|FD-Score:5.10|P-value:1.39E-5 ID:SGTC_3237|Compound:9133362|FD-Score:4.16|P-value:3.26E-4 ID:SGTC_3339|Compound:9145883|FD-Score:-3.80|P-value:5.91E-4

Top fitness defect scores for YGR063C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2845 9003024 17.90 43.0 µM 2.26E-50 amide catabolism 2 6.93E-72
2 SGTC_1304 1165-0502 13.00 251.0 µM 2.04E-27 3 6.96E-39
3 SGTC_2980 9014342 12.20 25.3 µM 3.29E-24 3 2.86E-34
4 SGTC_2976 9009005 9.67 19.5 µM 5.64E-16 6 1.99E-22
5 SGTC_2978 9011784 7.22 43.0 µM 1.22E-9 2 2.56E-13
6 SGTC_134 0269-0018 7.06 6.9 µM 2.75E-9 66 8.25E-13
7 SGTC_543 3844-1095 6.98 191.0 µM 4.12E-9 ERG2 11 1.48E-12
8 SGTC_2604 4'-hydroxychalcone 6.69 11.3 µM 1.68E-8 4 1.12E-11
9 SGTC_1458 k015-0034 6.41 6.3 µM 6.24E-8 2 7.33E-11
10 SGTC_43 0570-0105 6.31 72.5 µM 9.59E-8 ERG2 31 1.36E-10
11 SGTC_2289 7946404 6.10 165.1 µM 2.52E-7 36 5.44E-10
12 SGTC_1173 0443-0020 5.72 122.0 µM 1.22E-6 TSC3-RPN4 11 5.25E-9
13 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 5.57 15.3 µM 2.30E-6 10 1.30E-8
14 SGTC_1687 st020778 5.46 55.4 µM 3.48E-6 121 2.36E-8
15 SGTC_3098 9119039 5.46 49.5 µM 3.54E-6 Golgi 10 2.42E-8
16 SGTC_241 5hr heat shock (37°C) + cantharidin 5.37 155.4 µM 5.05E-6 cell wall signaling 39 4.03E-8
17 SGTC_453 enantio-paf c16 5.32 10.0 µM 5.91E-6 77 5.05E-8
18 SGTC_1801 st050089 5.24 28.4 µM 8.05E-6 NEO1-PIK1 66 7.87E-8
19 SGTC_146 3448-8650 5.18 53.1 µM 1.01E-5 NEO1-PIK1 77 1.08E-7
20 SGTC_3200 9114385 5.10 49.5 µM 1.39E-5 12 1.73E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.277 2.52E-60 YGR064W_d YGR064W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
0.263 3.87E-54 YGL025C PGD1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.215 1.43E-36 YOL072W THP1 hom Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
0.210 1.01E-34 YOL051W GAL11 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
0.204 8.73E-33 YIR023W DAL81 hom Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
0.187 6.68E-28 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.169 5.58E-23 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.166 3.46E-22 YNL274C GOR1 hom Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
0.166 4.94E-22 YNL139C THO2 hom Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
0.164 1.67E-21 YDR159W SAC3 hom Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
0.156 9.48E-20 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.155 1.49E-19 YHR167W THP2 hom Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
0.148 7.04E-18 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.144 5.91E-17 YHL011C PRS3 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
0.139 8.43E-16 YML122C_d YML122C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data