HIPHOP chemogenomics database

YGR065C / VHT1

High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin

Zygosity: Heterozygous strain

fixedexpanded
Profile for YGR065C / VHT1

Click on Significant Values for Screen Details

Top fitness defect scores for YGR065C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_264	promethazine	13.70	12.5 mM	4.96E-47	DNA intercalators	2	3.53E-43	2.99
2	SGTC_145	3737-0113	6.51	38.4 µM	4.85E-12	DNA intercalators	13	3.77E-11	0.05
3	SGTC_867	0518-0118	6.33	155.0 µM	1.79E-11	DNA intercalators	4	1.25E-10	0.29
4	SGTC_1748	st037455	5.77	53.6 µM	8.04E-10		1	4.07E-9	0.81
5	SGTC_471	1-acyl-paf	5.24	4.0 µM	2.08E-8	DNA intercalators	11	8.01E-8	0.55
6	SGTC_1926	methyl fluorone black	5.16	53.6 µM	3.30E-8	DNA intercalators	14	1.22E-7	0.09
7	SGTC_2949	9072542	4.90	2.2 µM	1.46E-7	TSC3-RPN4	4	4.78E-7	0.10
8	SGTC_144	4161-2749	4.84	112.4 µM	2.05E-7	DNA intercalators	7	6.54E-7	0.09
9	SGTC_2444	5485484	4.83	36.4 µM	2.10E-7		3	6.68E-7	0.34
10	SGTC_1385	3091-4834	4.72	14.1 µM	3.94E-7	RPP1 & pyrimidine depletion	3	1.19E-6	0.17
11	SGTC_49	0717-0965	4.65	32.1 µM	5.73E-7	60S ribosome export	6	1.68E-6	0.42
12	SGTC_50	0929-0078	4.62	56.4 µM	6.50E-7		4	1.88E-6	0.33
13	SGTC_257	daunorubicin	4.51	18.5 µM	1.18E-6	anthracycline transcription coupled DNA repair	11	3.26E-6	0.04
14	SGTC_3182	9108849	4.39	49.5 µM	2.22E-6	RPP1 & pyrimidine depletion	2	5.79E-6	0.46
15	SGTC_2515	chrysin	4.10	20.8 µM	8.86E-6	RPP1 & pyrimidine depletion	6	2.07E-5	0.23
16	SGTC_393	acivicin	4.05	6.2 µM	1.14E-5		9	2.59E-5	0.02
17	SGTC_1515	3460-0031	4.00	42.1 µM	1.41E-5	DNA intercalators	7	3.16E-5	0.31
18	SGTC_1337	1486-1011	3.84	40.3 µM	2.96E-5		5	6.25E-5	0.54
19	SGTC_330	1488-0319	3.81	80.1 µM	3.31E-5	RPP1 & pyrimidine depletion	6	6.94E-5	0.03
20	SGTC_2945	9067681	3.80	61.8 µM	3.42E-5		5	7.14E-5	0.19

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.412	1.88E-137	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.407	5.17E-134	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.393	3.86E-124	YMR166C_p	YMR166C_p	hom	Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.391	1.37E-122	YDL223C	HBT1	hom	Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.389	1.83E-121	YMR289W	ABZ2	hom	Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.366	1.06E-106	YOR133W	EFT1	hom	Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.361	4.73E-104	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.345	1.52E-94	YJL025W	RRN7	het	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.310	1.49E-75	YEL032W	MCM3	het	Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.306	1.41E-73	YGL200C	EMP24	hom	Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.304	1.02E-72	YGL041C_d	YGL041C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.289	2.69E-65	YMR262W_p	YMR262W_p	hom	Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.270	4.37E-57	YHR172W	SPC97	het	Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.268	3.64E-56	YPL157W	TGS1	hom	Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.232	2.97E-42	YDL102W	POL3	het	Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)