YGR065C / VHT1

High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR065C / VHT1

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:3.39|P-value:1.89E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.63|P-value:7.12E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.06|P-value:6.88E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:4.65|P-value:5.73E-7 ID:SGTC_50|Compound:0929-0078|FD-Score:4.62|P-value:6.50E-7 ID:SGTC_52|Compound:0329-0193|FD-Score:-4.45|P-value:1.72E-6 ID:SGTC_62|Compound:0180-0423|FD-Score:3.67|P-value:6.08E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.60|P-value:8.33E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:4.84|P-value:2.05E-7 ID:SGTC_145|Compound:3737-0113|FD-Score:6.51|P-value:4.85E-12 ID:SGTC_166|Compound:k072-0058|FD-Score:2.98|P-value:8.94E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:4.51|P-value:1.18E-6 ID:SGTC_264|Compound:promethazine|FD-Score:13.70|P-value:4.96E-47 ID:SGTC_330|Compound:1488-0319|FD-Score:3.81|P-value:3.31E-5 ID:SGTC_393|Compound:acivicin|FD-Score:4.05|P-value:1.14E-5 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:-3.66|P-value:6.61E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.67|P-value:6.31E-5 ID:SGTC_419|Compound:nsc-638432|FD-Score:-3.03|P-value:7.92E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.52|P-value:1.15E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:5.24|P-value:2.08E-8 ID:SGTC_574|Compound:1315-0397|FD-Score:3.37|P-value:2.05E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.80|P-value:3.49E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:3.04|P-value:7.30E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.44|P-value:1.60E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:6.33|P-value:1.79E-11 ID:SGTC_874|Compound:0599-0792|FD-Score:3.26|P-value:3.25E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:-3.01|P-value:8.54E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.33|P-value:2.42E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.02|P-value:7.90E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.24|P-value:3.49E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:2.97|P-value:9.26E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.84|P-value:2.96E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.23|P-value:3.59E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.72|P-value:3.94E-7 ID:SGTC_1399|Compound:3562-5772|FD-Score:3.51|P-value:1.20E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.23|P-value:3.64E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.30|P-value:2.70E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:4.00|P-value:1.41E-5 ID:SGTC_1519|Compound:esterone|FD-Score:3.33|P-value:2.46E-4 ID:SGTC_1706|Compound:st031321|FD-Score:2.97|P-value:9.30E-4 ID:SGTC_1748|Compound:st037455|FD-Score:5.77|P-value:8.04E-10 ID:SGTC_1847|Compound:st056231|FD-Score:3.00|P-value:8.47E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.12|P-value:5.35E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.16|P-value:3.30E-8 ID:SGTC_2055|Compound:5235184|FD-Score:-3.53|P-value:1.16E-4 ID:SGTC_2354|Compound:9060492|FD-Score:3.68|P-value:5.88E-5 ID:SGTC_2360|Compound:9040895|FD-Score:-3.40|P-value:1.96E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.58|P-value:8.91E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.08|P-value:6.34E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.83|P-value:2.10E-7 ID:SGTC_2515|Compound:chrysin|FD-Score:4.10|P-value:8.86E-6 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-7.88|P-value:9.26E-17 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.10|P-value:6.11E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.20|P-value:4.05E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-4.03|P-value:1.30E-5 ID:SGTC_2724|Compound:maprotiline|FD-Score:-3.45|P-value:1.60E-4 ID:SGTC_2732|Compound:terfenadine|FD-Score:3.61|P-value:7.84E-5 ID:SGTC_2748|Compound:diazoxide|FD-Score:-3.05|P-value:7.41E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.50|P-value:1.22E-4 ID:SGTC_2923|Compound:7992654|FD-Score:3.31|P-value:2.69E-4 ID:SGTC_2945|Compound:9067681|FD-Score:3.80|P-value:3.42E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-3.39|P-value:2.05E-4 ID:SGTC_2949|Compound:9072542|FD-Score:4.90|P-value:1.46E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.24|P-value:3.48E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.98|P-value:1.60E-5 ID:SGTC_3108|Compound:9121785|FD-Score:-4.20|P-value:5.95E-6 ID:SGTC_3182|Compound:9108849|FD-Score:4.39|P-value:2.22E-6 ID:SGTC_3338|Compound:9144300|FD-Score:2.99|P-value:8.74E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:3.39|P-value:1.89E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.63|P-value:7.12E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.06|P-value:6.88E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:4.65|P-value:5.73E-7 ID:SGTC_50|Compound:0929-0078|FD-Score:4.62|P-value:6.50E-7 ID:SGTC_52|Compound:0329-0193|FD-Score:-4.45|P-value:1.72E-6 ID:SGTC_62|Compound:0180-0423|FD-Score:3.67|P-value:6.08E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.60|P-value:8.33E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:4.84|P-value:2.05E-7 ID:SGTC_145|Compound:3737-0113|FD-Score:6.51|P-value:4.85E-12 ID:SGTC_166|Compound:k072-0058|FD-Score:2.98|P-value:8.94E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:4.51|P-value:1.18E-6 ID:SGTC_264|Compound:promethazine|FD-Score:13.70|P-value:4.96E-47 ID:SGTC_330|Compound:1488-0319|FD-Score:3.81|P-value:3.31E-5 ID:SGTC_393|Compound:acivicin|FD-Score:4.05|P-value:1.14E-5 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:-3.66|P-value:6.61E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.67|P-value:6.31E-5 ID:SGTC_419|Compound:nsc-638432|FD-Score:-3.03|P-value:7.92E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.52|P-value:1.15E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:5.24|P-value:2.08E-8 ID:SGTC_574|Compound:1315-0397|FD-Score:3.37|P-value:2.05E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.80|P-value:3.49E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:3.04|P-value:7.30E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.44|P-value:1.60E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:6.33|P-value:1.79E-11 ID:SGTC_874|Compound:0599-0792|FD-Score:3.26|P-value:3.25E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:-3.01|P-value:8.54E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.33|P-value:2.42E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.02|P-value:7.90E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.24|P-value:3.49E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:2.97|P-value:9.26E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.84|P-value:2.96E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.23|P-value:3.59E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.72|P-value:3.94E-7 ID:SGTC_1399|Compound:3562-5772|FD-Score:3.51|P-value:1.20E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.23|P-value:3.64E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.30|P-value:2.70E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:4.00|P-value:1.41E-5 ID:SGTC_1519|Compound:esterone|FD-Score:3.33|P-value:2.46E-4 ID:SGTC_1706|Compound:st031321|FD-Score:2.97|P-value:9.30E-4 ID:SGTC_1748|Compound:st037455|FD-Score:5.77|P-value:8.04E-10 ID:SGTC_1847|Compound:st056231|FD-Score:3.00|P-value:8.47E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.12|P-value:5.35E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.16|P-value:3.30E-8 ID:SGTC_2055|Compound:5235184|FD-Score:-3.53|P-value:1.16E-4 ID:SGTC_2354|Compound:9060492|FD-Score:3.68|P-value:5.88E-5 ID:SGTC_2360|Compound:9040895|FD-Score:-3.40|P-value:1.96E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.58|P-value:8.91E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.08|P-value:6.34E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.83|P-value:2.10E-7 ID:SGTC_2515|Compound:chrysin|FD-Score:4.10|P-value:8.86E-6 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-7.88|P-value:9.26E-17 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.10|P-value:6.11E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.20|P-value:4.05E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-4.03|P-value:1.30E-5 ID:SGTC_2724|Compound:maprotiline|FD-Score:-3.45|P-value:1.60E-4 ID:SGTC_2732|Compound:terfenadine|FD-Score:3.61|P-value:7.84E-5 ID:SGTC_2748|Compound:diazoxide|FD-Score:-3.05|P-value:7.41E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.50|P-value:1.22E-4 ID:SGTC_2923|Compound:7992654|FD-Score:3.31|P-value:2.69E-4 ID:SGTC_2945|Compound:9067681|FD-Score:3.80|P-value:3.42E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-3.39|P-value:2.05E-4 ID:SGTC_2949|Compound:9072542|FD-Score:4.90|P-value:1.46E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.24|P-value:3.48E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.98|P-value:1.60E-5 ID:SGTC_3108|Compound:9121785|FD-Score:-4.20|P-value:5.95E-6 ID:SGTC_3182|Compound:9108849|FD-Score:4.39|P-value:2.22E-6 ID:SGTC_3338|Compound:9144300|FD-Score:2.99|P-value:8.74E-4

Top fitness defect scores for YGR065C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_264 promethazine 13.70 12.5 mM 4.96E-47 DNA intercalators 2 3.53E-43 2.99
2 SGTC_145 3737-0113 6.51 38.4 µM 4.85E-12 DNA intercalators 13 3.77E-11 0.05
3 SGTC_867 0518-0118 6.33 155.0 µM 1.79E-11 DNA intercalators 4 1.25E-10 0.29
4 SGTC_1748 st037455 5.77 53.6 µM 8.04E-10 1 4.07E-9 0.81
5 SGTC_471 1-acyl-paf 5.24 4.0 µM 2.08E-8 DNA intercalators 11 8.01E-8 0.55
6 SGTC_1926 methyl fluorone black 5.16 53.6 µM 3.30E-8 DNA intercalators 14 1.22E-7 0.09
7 SGTC_2949 9072542 4.90 2.2 µM 1.46E-7 TSC3-RPN4 4 4.78E-7 0.10
8 SGTC_144 4161-2749 4.84 112.4 µM 2.05E-7 DNA intercalators 7 6.54E-7 0.09
9 SGTC_2444 5485484 4.83 36.4 µM 2.10E-7 3 6.68E-7 0.34
10 SGTC_1385 3091-4834 4.72 14.1 µM 3.94E-7 RPP1 & pyrimidine depletion 3 1.19E-6 0.17
11 SGTC_49 0717-0965 4.65 32.1 µM 5.73E-7 60S ribosome export 6 1.68E-6 0.42
12 SGTC_50 0929-0078 4.62 56.4 µM 6.50E-7 4 1.88E-6 0.33
13 SGTC_257 daunorubicin 4.51 18.5 µM 1.18E-6 anthracycline transcription coupled DNA repair 11 3.26E-6 0.04
14 SGTC_3182 9108849 4.39 49.5 µM 2.22E-6 RPP1 & pyrimidine depletion 2 5.79E-6 0.46
15 SGTC_2515 chrysin 4.10 20.8 µM 8.86E-6 RPP1 & pyrimidine depletion 6 2.07E-5 0.23
16 SGTC_393 acivicin 4.05 6.2 µM 1.14E-5 9 2.59E-5 0.02
17 SGTC_1515 3460-0031 4.00 42.1 µM 1.41E-5 DNA intercalators 7 3.16E-5 0.31
18 SGTC_1337 1486-1011 3.84 40.3 µM 2.96E-5 5 6.25E-5 0.54
19 SGTC_330 1488-0319 3.81 80.1 µM 3.31E-5 RPP1 & pyrimidine depletion 6 6.94E-5 0.03
20 SGTC_2945 9067681 3.80 61.8 µM 3.42E-5 5 7.14E-5 0.19

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.412 1.88E-137 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.407 5.17E-134 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.393 3.86E-124 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.391 1.37E-122 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.389 1.83E-121 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.366 1.06E-106 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.361 4.73E-104 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.345 1.52E-94 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.310 1.49E-75 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.306 1.41E-73 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.304 1.02E-72 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.289 2.69E-65 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.270 4.37E-57 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.268 3.64E-56 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.232 2.97E-42 YDL102W POL3 het Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)