YGR074W / SMD1

Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR074W / SMD1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.08|P-value:5.16E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.87|P-value:1.45E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:-4.12|P-value:5.20E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.60|P-value:5.96E-5 ID:SGTC_230|Compound:MPP+|FD-Score:2.94|P-value:7.24E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.00|P-value:5.90E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:-3.31|P-value:2.02E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.02|P-value:8.80E-6 ID:SGTC_376|Compound:3970-0862|FD-Score:5.79|P-value:2.22E-10 ID:SGTC_532|Compound:1548-0774|FD-Score:3.32|P-value:1.70E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.39|P-value:3.18E-9 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.05|P-value:4.79E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.22|P-value:2.98E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-2.99|P-value:7.15E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.40|P-value:1.21E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.04|P-value:6.45E-6 ID:SGTC_1486|Compound:3970-0793|FD-Score:-3.70|P-value:3.94E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:-3.28|P-value:2.32E-4 ID:SGTC_1617|Compound:st002906|FD-Score:-3.52|P-value:8.57E-5 ID:SGTC_1621|Compound:st003705|FD-Score:3.33|P-value:1.59E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.41|P-value:1.15E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.73|P-value:2.82E-5 ID:SGTC_1984|Compound:st072354|FD-Score:-3.02|P-value:6.28E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-3.63|P-value:5.31E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.31|P-value:1.62E-6 ID:SGTC_2442|Compound:5158906|FD-Score:-3.17|P-value:3.60E-4 ID:SGTC_2700|Compound:st077718|FD-Score:2.86|P-value:9.83E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-3.43|P-value:1.27E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.36|P-value:1.70E-4 ID:SGTC_3025|Compound:9087895|FD-Score:-4.08|P-value:6.47E-6 ID:SGTC_3033|Compound:9090887|FD-Score:3.13|P-value:3.50E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.93|P-value:1.31E-5 ID:SGTC_3076|Compound:9116187|FD-Score:-4.60|P-value:4.47E-7 ID:SGTC_3106|Compound:9121286|FD-Score:3.90|P-value:1.30E-5 ID:SGTC_3178|Compound:9107506|FD-Score:-3.22|P-value:2.93E-4 ID:SGTC_3278|Compound:9139605|FD-Score:-3.00|P-value:6.81E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.08|P-value:5.16E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.87|P-value:1.45E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:-4.12|P-value:5.20E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.60|P-value:5.96E-5 ID:SGTC_230|Compound:MPP+|FD-Score:2.94|P-value:7.24E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.00|P-value:5.90E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:-3.31|P-value:2.02E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.02|P-value:8.80E-6 ID:SGTC_376|Compound:3970-0862|FD-Score:5.79|P-value:2.22E-10 ID:SGTC_532|Compound:1548-0774|FD-Score:3.32|P-value:1.70E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.39|P-value:3.18E-9 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.05|P-value:4.79E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.22|P-value:2.98E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-2.99|P-value:7.15E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.40|P-value:1.21E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:4.04|P-value:6.45E-6 ID:SGTC_1486|Compound:3970-0793|FD-Score:-3.70|P-value:3.94E-5 ID:SGTC_1502|Compound:4534-4282|FD-Score:-3.28|P-value:2.32E-4 ID:SGTC_1617|Compound:st002906|FD-Score:-3.52|P-value:8.57E-5 ID:SGTC_1621|Compound:st003705|FD-Score:3.33|P-value:1.59E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.41|P-value:1.15E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.73|P-value:2.82E-5 ID:SGTC_1984|Compound:st072354|FD-Score:-3.02|P-value:6.28E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-3.63|P-value:5.31E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.31|P-value:1.62E-6 ID:SGTC_2442|Compound:5158906|FD-Score:-3.17|P-value:3.60E-4 ID:SGTC_2700|Compound:st077718|FD-Score:2.86|P-value:9.83E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-3.43|P-value:1.27E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.36|P-value:1.70E-4 ID:SGTC_3025|Compound:9087895|FD-Score:-4.08|P-value:6.47E-6 ID:SGTC_3033|Compound:9090887|FD-Score:3.13|P-value:3.50E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.93|P-value:1.31E-5 ID:SGTC_3076|Compound:9116187|FD-Score:-4.60|P-value:4.47E-7 ID:SGTC_3106|Compound:9121286|FD-Score:3.90|P-value:1.30E-5 ID:SGTC_3178|Compound:9107506|FD-Score:-3.22|P-value:2.93E-4 ID:SGTC_3278|Compound:9139605|FD-Score:-3.00|P-value:6.81E-4

Top fitness defect scores for YGR074W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_376 3970-0862 5.79 59.1 µM 2.22E-10 3 3.60E-9 1.34
2 SGTC_1129 3453-1384 5.39 223.0 µM 3.18E-9 7 3.62E-8 0.26
3 SGTC_2011 4012611 4.31 177.0 µM 1.62E-6 mitochondrial processes 8 8.08E-6 0.19
4 SGTC_1394 3474-0001 4.04 65.7 µM 6.45E-6 endomembrane recycling 3 2.68E-5 0.16
5 SGTC_3106 9121286 3.90 49.5 µM 1.30E-5 3 4.91E-5 0.21
6 SGTC_148 3253-0744 3.87 288.0 µM 1.45E-5 9 5.39E-5 0.05
7 SGTC_1747 st037496 3.73 66.6 µM 2.82E-5 10 9.64E-5 0.10
8 SGTC_1742 st038235 3.41 28.9 µM 1.15E-4 mitochondrial stress 13 3.28E-4 0.00
9 SGTC_1393 3470-5649 3.40 58.2 µM 1.21E-4 60S ribosome export 6 3.43E-4 0.17
10 SGTC_1621 st003705 3.33 32.4 µM 1.59E-4 calcium & mitochondrial duress 12 4.32E-4 0.14
11 SGTC_532 1548-0774 3.32 215.0 µM 1.70E-4 8 4.58E-4 0.05
12 SGTC_3033 9090887 3.13 49.5 µM 3.50E-4 fatty acid desaturase (OLE1) 31 8.60E-4 0.06
13 SGTC_1171 0368-0077 3.05 21.4 µM 4.79E-4 28 0.00113 0.01
14 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.00 8.9 µM 5.90E-4 heat shock/prefoldin 20 0.00135 0.07
15 SGTC_230 MPP+ 2.94 141.7 µM 7.24E-4 18 0.00162 0.02
16 SGTC_2700 st077718 2.86 96.8 µM 9.83E-4 16 0.00211 0.01
17 SGTC_385 1494-0393 2.83 12.4 µM 0.00110 18 0.00233 0.04
18 SGTC_1130 3454-2858 2.74 15.9 µM 0.00150 NEO1 33 0.00305 0.02
19 SGTC_1322 1348-1070 2.72 12.0 µM 0.00160 12 0.00324 0.04
20 SGTC_3003 9075612 2.69 71.4 µM 0.00182 19 0.00361 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.40E-13 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.114 3.09E-11 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.108 3.44E-10 YIL118W RHO3 het Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
0.104 1.30E-9 YPR186C PZF1 het Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
0.095 3.36E-8 YKR010C TOF2 hom Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
0.094 4.98E-8 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.092 8.86E-8 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.088 2.89E-7 YIL038C NOT3 hom Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
0.088 3.31E-7 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.088 3.35E-7 YGL260W_p YGL260W_p hom Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium
0.085 7.26E-7 YLR363W-A YLR363W-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
0.084 1.12E-6 YLR276C DBP9 het DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
0.084 1.12E-6 YLR439W MRPL4 hom Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
0.081 2.37E-6 YNL109W_d YNL109W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C
0.079 4.40E-6 YDR441C APT2 hom Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication