YGR076C / MRPL25

Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR076C / MRPL25

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.51|P-value:1.16E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:4.15|P-value:4.87E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:4.76|P-value:3.95E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:10.60|P-value:1.08E-23 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.44|P-value:5.87E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:5.65|P-value:5.56E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:4.33|P-value:2.40E-5 ID:SGTC_218|Compound:alverine citrate|FD-Score:5.02|P-value:1.20E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:15.70|P-value:2.09E-49 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.88|P-value:1.35E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:9.26|P-value:1.58E-18 ID:SGTC_386|Compound:1509-0011|FD-Score:13.80|P-value:1.25E-38 ID:SGTC_595|Compound:3909-8003|FD-Score:4.39|P-value:1.92E-5 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.68|P-value:2.56E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.70|P-value:2.37E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:4.18|P-value:4.44E-5 ID:SGTC_684|Compound:1140-0066|FD-Score:-3.55|P-value:4.03E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.85|P-value:1.52E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:-5.53|P-value:9.41E-8 ID:SGTC_908|Compound:3448-7491|FD-Score:4.08|P-value:6.39E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:3.73|P-value:2.31E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.89|P-value:2.25E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:5.71|P-value:4.13E-8 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.74|P-value:4.33E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.34|P-value:8.65E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.77|P-value:2.03E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.53|P-value:4.60E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:5.73|P-value:3.65E-8 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.00|P-value:8.59E-5 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:4.62|P-value:7.15E-6 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-3.48|P-value:5.05E-4 ID:SGTC_1635|Compound:st007035|FD-Score:15.60|P-value:9.21E-49 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:4.50|P-value:1.19E-5 ID:SGTC_1711|Compound:st032263|FD-Score:3.83|P-value:1.64E-4 ID:SGTC_1717|Compound:st033143|FD-Score:5.99|P-value:9.03E-9 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.93|P-value:1.06E-4 ID:SGTC_1820|Compound:st053765|FD-Score:4.07|P-value:6.74E-5 ID:SGTC_1983|Compound:st071813|FD-Score:5.65|P-value:5.71E-8 ID:SGTC_2187|Compound:6125751|FD-Score:3.31|P-value:9.43E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.32|P-value:9.29E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.63|P-value:3.35E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.63|P-value:3.28E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.75|P-value:2.16E-4 ID:SGTC_2785|Compound:7756463|FD-Score:4.49|P-value:1.27E-5 ID:SGTC_2916|Compound:7875993|FD-Score:-4.25|P-value:3.08E-5 ID:SGTC_2947|Compound:9072639|FD-Score:3.93|P-value:1.13E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-4.09|P-value:5.77E-5 ID:SGTC_2983|Compound:9022671|FD-Score:3.63|P-value:3.34E-4 ID:SGTC_3188|Compound:9110789|FD-Score:-3.29|P-value:9.56E-4 ID:SGTC_3202|Compound:9113761|FD-Score:6.12|P-value:4.37E-9 ID:SGTC_3206|Compound:9114350|FD-Score:3.75|P-value:2.17E-4 ID:SGTC_3223|Compound:9129970|FD-Score:5.44|P-value:1.65E-7 ID:SGTC_3224|Compound:9130643|FD-Score:4.38|P-value:1.94E-5 ID:SGTC_3288|Compound:9119690|FD-Score:-4.06|P-value:6.39E-5 ID:SGTC_3289|Compound:9119912|FD-Score:3.93|P-value:1.11E-4 ID:SGTC_3293|Compound:9120939|FD-Score:-3.77|P-value:1.87E-4 ID:SGTC_3296|Compound:9122258|FD-Score:-3.61|P-value:3.32E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.51|P-value:1.16E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:4.15|P-value:4.87E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:4.76|P-value:3.95E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:10.60|P-value:1.08E-23 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.44|P-value:5.87E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:5.65|P-value:5.56E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:4.33|P-value:2.40E-5 ID:SGTC_218|Compound:alverine citrate|FD-Score:5.02|P-value:1.20E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:15.70|P-value:2.09E-49 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.88|P-value:1.35E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:9.26|P-value:1.58E-18 ID:SGTC_386|Compound:1509-0011|FD-Score:13.80|P-value:1.25E-38 ID:SGTC_595|Compound:3909-8003|FD-Score:4.39|P-value:1.92E-5 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.68|P-value:2.56E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.70|P-value:2.37E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:4.18|P-value:4.44E-5 ID:SGTC_684|Compound:1140-0066|FD-Score:-3.55|P-value:4.03E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.85|P-value:1.52E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:-5.53|P-value:9.41E-8 ID:SGTC_908|Compound:3448-7491|FD-Score:4.08|P-value:6.39E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:3.73|P-value:2.31E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.89|P-value:2.25E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:5.71|P-value:4.13E-8 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.74|P-value:4.33E-6 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.34|P-value:8.65E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.77|P-value:2.03E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.53|P-value:4.60E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:5.73|P-value:3.65E-8 ID:SGTC_1478|Compound:0453-0656|FD-Score:4.00|P-value:8.59E-5 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:4.62|P-value:7.15E-6 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-3.48|P-value:5.05E-4 ID:SGTC_1635|Compound:st007035|FD-Score:15.60|P-value:9.21E-49 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:4.50|P-value:1.19E-5 ID:SGTC_1711|Compound:st032263|FD-Score:3.83|P-value:1.64E-4 ID:SGTC_1717|Compound:st033143|FD-Score:5.99|P-value:9.03E-9 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.93|P-value:1.06E-4 ID:SGTC_1820|Compound:st053765|FD-Score:4.07|P-value:6.74E-5 ID:SGTC_1983|Compound:st071813|FD-Score:5.65|P-value:5.71E-8 ID:SGTC_2187|Compound:6125751|FD-Score:3.31|P-value:9.43E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.32|P-value:9.29E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.63|P-value:3.35E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.63|P-value:3.28E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.75|P-value:2.16E-4 ID:SGTC_2785|Compound:7756463|FD-Score:4.49|P-value:1.27E-5 ID:SGTC_2916|Compound:7875993|FD-Score:-4.25|P-value:3.08E-5 ID:SGTC_2947|Compound:9072639|FD-Score:3.93|P-value:1.13E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-4.09|P-value:5.77E-5 ID:SGTC_2983|Compound:9022671|FD-Score:3.63|P-value:3.34E-4 ID:SGTC_3188|Compound:9110789|FD-Score:-3.29|P-value:9.56E-4 ID:SGTC_3202|Compound:9113761|FD-Score:6.12|P-value:4.37E-9 ID:SGTC_3206|Compound:9114350|FD-Score:3.75|P-value:2.17E-4 ID:SGTC_3223|Compound:9129970|FD-Score:5.44|P-value:1.65E-7 ID:SGTC_3224|Compound:9130643|FD-Score:4.38|P-value:1.94E-5 ID:SGTC_3288|Compound:9119690|FD-Score:-4.06|P-value:6.39E-5 ID:SGTC_3289|Compound:9119912|FD-Score:3.93|P-value:1.11E-4 ID:SGTC_3293|Compound:9120939|FD-Score:-3.77|P-value:1.87E-4 ID:SGTC_3296|Compound:9122258|FD-Score:-3.61|P-value:3.32E-4

Top fitness defect scores for YGR076C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 15.70 5.3 µM 2.09E-49 92 1.35E-55
2 SGTC_1635 st007035 15.60 42.4 µM 9.21E-49 33 7.22E-55
3 SGTC_386 1509-0011 13.80 3.1 µM 1.25E-38 26 2.01E-43
4 SGTC_134 0269-0018 10.60 6.9 µM 1.08E-23 36 1.47E-26
5 SGTC_275 trichlorophene 9.26 5.7 µM 1.58E-18 mitochondrial stress 54 1.00E-20
6 SGTC_3202 9113761 6.12 49.5 µM 4.37E-9 11 4.54E-10
7 SGTC_1717 st033143 5.99 58.0 µM 9.03E-9 10 1.03E-9
8 SGTC_1455 4262-0120 5.73 131.0 µM 3.65E-8 plasma membrane duress 9 4.97E-9
9 SGTC_1049 3226-0134 5.71 66.0 µM 4.13E-8 5 5.71E-9
10 SGTC_161 4065-0411 5.65 162.2 µM 5.56E-8 32 7.98E-9
11 SGTC_1983 st071813 5.65 46.9 µM 5.71E-8 RPP1 & pyrimidine depletion 2 8.23E-9
12 SGTC_3223 9129970 5.44 49.5 µM 1.65E-7 3 2.73E-8
13 SGTC_218 alverine citrate 5.02 93.8 µM 1.20E-6 fatty acid desaturase (OLE1) 20 2.55E-7
14 SGTC_1002 4049-0206 4.89 172.0 µM 2.25E-6 11 5.16E-7
15 SGTC_119 0121-0043 4.76 24.8 µM 3.95E-6 mitochondrial stress 68 9.76E-7
16 SGTC_1188 1403-0014 4.74 4.6 µM 4.33E-6 mitochondrial stress 33 1.08E-6
17 SGTC_1580 hydrocortisone 21-hemisuccinate 4.62 43.2 µM 7.15E-6 8 1.90E-6
18 SGTC_10 0279-0212 4.51 42.6 µM 1.16E-5 TRP & mitochondrial translation 94 3.29E-6
19 SGTC_1692 fluoroquinolonic acid 4.50 71.0 µM 1.19E-5 1 3.37E-6
20 SGTC_2785 7756463 4.49 72.7 µM 1.27E-5 15 3.63E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.604 0 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.572 9.43E-291 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.570 4.59E-288 YJL022W_d YJL022W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.562 7.10E-279 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.558 7.96E-274 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.557 7.68E-273 YKL194C MST1 hom Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
0.554 2.64E-269 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.554 1.33E-269 YDR231C COX20 hom Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.554 2.70E-269 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.554 4.53E-269 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.548 3.52E-262 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.547 1.79E-261 YNL213C RRG9 hom Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.543 2.80E-257 YNR045W PET494 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
0.538 6.40E-251 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.537 5.52E-250 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase