YGR080W / TWF1

Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR080W / TWF1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-3.22|P-value:3.15E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.05|P-value:9.29E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:5.05|P-value:4.67E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.00|P-value:7.47E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:5.21|P-value:1.81E-8 ID:SGTC_324|Compound:1315-0088|FD-Score:5.25|P-value:1.42E-8 ID:SGTC_333|Compound:3486-0131|FD-Score:3.35|P-value:2.01E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.00|P-value:7.68E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.26|P-value:2.80E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.05|P-value:6.09E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.33|P-value:2.12E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.05|P-value:6.35E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:2.97|P-value:8.42E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:2.98|P-value:8.14E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.11|P-value:4.99E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:-3.04|P-value:6.30E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.53|P-value:8.09E-7 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.23|P-value:3.08E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.68|P-value:4.92E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.26|P-value:2.77E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.10|P-value:5.24E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.46|P-value:1.27E-4 ID:SGTC_2084|Compound:5306078|FD-Score:4.05|P-value:9.36E-6 ID:SGTC_2098|Compound:5376439|FD-Score:3.23|P-value:3.23E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.13|P-value:4.70E-4 ID:SGTC_2239|Compound:6685329|FD-Score:-2.93|P-value:9.65E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.35|P-value:1.94E-4 ID:SGTC_2261|Compound:9010538|FD-Score:-3.08|P-value:5.38E-4 ID:SGTC_2359|Compound:9039622|FD-Score:-3.19|P-value:3.62E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-2.99|P-value:7.71E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.42|P-value:1.49E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-2.96|P-value:8.39E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.47|P-value:1.22E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.39|P-value:1.62E-4 ID:SGTC_2852|Compound:9023854|FD-Score:-3.54|P-value:8.78E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.32|P-value:2.20E-4 ID:SGTC_3004|Compound:9076122|FD-Score:3.06|P-value:6.08E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.00|P-value:7.59E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.14|P-value:4.34E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-2.95|P-value:8.71E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-2.93|P-value:9.31E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-2.97|P-value:8.25E-4 ID:SGTC_3186|Compound:9110464|FD-Score:4.82|P-value:1.67E-7 ID:SGTC_3213|Compound:9129884|FD-Score:-3.37|P-value:1.79E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.81|P-value:2.80E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-3.59|P-value:7.00E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.22|P-value:3.15E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.05|P-value:9.29E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:5.05|P-value:4.67E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.00|P-value:7.47E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:5.21|P-value:1.81E-8 ID:SGTC_324|Compound:1315-0088|FD-Score:5.25|P-value:1.42E-8 ID:SGTC_333|Compound:3486-0131|FD-Score:3.35|P-value:2.01E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.00|P-value:7.68E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.26|P-value:2.80E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.05|P-value:6.09E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.33|P-value:2.12E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.05|P-value:6.35E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:2.97|P-value:8.42E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:2.98|P-value:8.14E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.11|P-value:4.99E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:-3.04|P-value:6.30E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.53|P-value:8.09E-7 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.23|P-value:3.08E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.68|P-value:4.92E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.26|P-value:2.77E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.10|P-value:5.24E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.46|P-value:1.27E-4 ID:SGTC_2084|Compound:5306078|FD-Score:4.05|P-value:9.36E-6 ID:SGTC_2098|Compound:5376439|FD-Score:3.23|P-value:3.23E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.13|P-value:4.70E-4 ID:SGTC_2239|Compound:6685329|FD-Score:-2.93|P-value:9.65E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.35|P-value:1.94E-4 ID:SGTC_2261|Compound:9010538|FD-Score:-3.08|P-value:5.38E-4 ID:SGTC_2359|Compound:9039622|FD-Score:-3.19|P-value:3.62E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-2.99|P-value:7.71E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.42|P-value:1.49E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-2.96|P-value:8.39E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.47|P-value:1.22E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.39|P-value:1.62E-4 ID:SGTC_2852|Compound:9023854|FD-Score:-3.54|P-value:8.78E-5 ID:SGTC_2880|Compound:9042983|FD-Score:3.32|P-value:2.20E-4 ID:SGTC_3004|Compound:9076122|FD-Score:3.06|P-value:6.08E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.00|P-value:7.59E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.14|P-value:4.34E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-2.95|P-value:8.71E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-2.93|P-value:9.31E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-2.97|P-value:8.25E-4 ID:SGTC_3186|Compound:9110464|FD-Score:4.82|P-value:1.67E-7 ID:SGTC_3213|Compound:9129884|FD-Score:-3.37|P-value:1.79E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.81|P-value:2.80E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-3.59|P-value:7.00E-5

Top fitness defect scores for YGR080W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_324 1315-0088 5.25 19.6 µM 1.42E-8 RSC & ERG11 1 7.74E-8
2 SGTC_322 k048-0136 5.21 19.3 µM 1.81E-8 RSC & ERG11 6 9.60E-8
3 SGTC_43 0570-0105 5.05 72.5 µM 4.67E-8 ERG2 53 2.24E-7
4 SGTC_3186 9110464 4.82 49.5 µM 1.67E-7 RPP1 & pyrimidine depletion 4 7.04E-7
5 SGTC_815 1582-0027 4.53 36.9 µM 8.09E-7 13 2.89E-6
6 SGTC_42 0133-0086 4.05 60.6 µM 9.29E-6 30 2.58E-5
7 SGTC_2084 5306078 4.05 60.6 µM 9.36E-6 18 2.60E-5
8 SGTC_3304 9125678 3.81 46.1 µM 2.80E-5 56 6.95E-5
9 SGTC_1112 tnp00069 3.68 1.2 µM 4.92E-5 29 1.16E-4
10 SGTC_2619 obliquin 3.47 87.9 µM 1.22E-4 cell wall 178 2.60E-4
11 SGTC_1733 st037357 3.46 54.9 µM 1.27E-4 60S ribosome export 55 2.71E-4
12 SGTC_2428 5615643 3.42 7.8 µM 1.49E-4 43 3.13E-4
13 SGTC_333 3486-0131 3.35 40.1 µM 2.01E-4 amide catabolism 23 4.09E-4
14 SGTC_497 capsazepine 3.33 133.0 µM 2.12E-4 28 4.30E-4
15 SGTC_2880 9042983 3.32 58.4 µM 2.20E-4 fatty acid desaturase (OLE1) 48 4.43E-4
16 SGTC_346 4513-0042 3.26 7.2 µM 2.80E-4 azole & statin 66 5.52E-4
17 SGTC_2098 5376439 3.23 882.5 nM 3.23E-4 31 6.26E-4
18 SGTC_2111 5486399 3.13 200.0 µM 4.70E-4 97 8.78E-4
19 SGTC_768 0384-0033 3.11 77.3 µM 4.99E-4 fatty acid desaturase (OLE1) 136 9.28E-4
20 SGTC_1724 st037281 3.10 8.3 µM 5.24E-4 95 9.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.139 5.17E-16 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.137 1.84E-15 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.136 3.05E-15 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.122 1.06E-12 YOL054W PSH1 hom E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p
0.118 6.06E-12 YLR229C CDC42 het Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
0.110 1.54E-10 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.106 6.78E-10 YDL139C SCM3 het Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP
0.105 1.35E-9 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.100 6.61E-9 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.095 3.28E-8 YGL154C LYS5 hom Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
0.095 3.29E-8 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.094 5.06E-8 YLR153C ACS2 het Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
0.094 5.72E-8 YDR400W URH1 hom Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
0.093 6.12E-8 YIL126W STH1 het ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
0.093 7.71E-8 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress