HIPHOP chemogenomics database

YGR083C / GCD2

Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain

fixedexpanded
Profile for YGR083C / GCD2

Click on Significant Values for Screen Details

Top fitness defect scores for YGR083C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2411	st077133	21.40	39.9 µM	2.12E-71	RNA pol III & RNase P/MRP	3	1.82E-101	0.64
2	SGTC_87	0100-0052	18.30	35.5 µM	3.87E-53		2	2.65E-75	7.28
3	SGTC_392	N-ethylmaleimide	14.60	8.3 µM	2.81E-34	RNA pol III & RNase P/MRP	2	2.71E-48	5.03
4	SGTC_1872	amphotericin b	14.10	148.0 nM	3.54E-32	RNA pol III & RNase P/MRP	4	2.75E-45	2.19
5	SGTC_2612	dictamnine	13.00	15.5 µM	1.06E-27	RNA pol III & RNase P/MRP	7	6.96E-39	3.18
6	SGTC_539	4859-0462	7.57	2.5 µM	1.33E-10	RNA pol III & RNase P/MRP	6	1.89E-14	0.07
7	SGTC_153	4077-0274	7.15	519.7 µM	1.17E-9		1	4.19E-13	0.96
8	SGTC_177	1327-0068	7.00	8.4 µM	2.62E-9		6	1.32E-12	0.78
9	SGTC_81	0411-0046	6.98	10.0 µM	2.86E-9		15	1.50E-12	0.01
10	SGTC_567	1080-0542	6.21	68.4 µM	1.08E-7		1	2.60E-10	1.93
11	SGTC_2781	5567276	5.96	71.4 µM	3.25E-7		2	1.25E-9	0.64
12	SGTC_611	3453-1652	5.71	23.7 µM	9.47E-7		10	5.69E-9	0.55
13	SGTC_130	0136-0219	5.66	337.7 µM	1.18E-6		1	7.76E-9	0.66
14	SGTC_1568	digitonin	5.27	820.0 nM	5.37E-6	sphingolipid biosynthesis & PDR1	5	6.66E-8	0.17
15	SGTC_318	1488-0629	5.26	67.2 µM	5.68E-6	copper-dependent oxidative stress	1	7.21E-8	0.78
16	SGTC_2323	6148992	5.26	148.3 µM	5.69E-6		8	7.22E-8	0.56
17	SGTC_1904	st060225	5.20	78.0 µM	7.09E-6		1	9.87E-8	0.53
18	SGTC_690	glyoxal	5.07	20.3 µM	1.16E-5		17	1.99E-7	0.08
19	SGTC_274	sirtinol	4.89	40.1 µM	2.21E-5		10	4.93E-7	0.21
20	SGTC_1768	st044301	4.86	37.2 µM	2.46E-5		1	5.73E-7	0.30

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.515	1.08E-226	YLR291C	GCD7	het	Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.411	4.60E-137	YNL221C	POP1	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.406	5.17E-133	YOR260W	GCD1	het	Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.310	1.86E-75	YDR211W	GCD6	het	Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.244	1.44E-46	YBR167C	POP7	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.242	7.95E-46	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.201	6.75E-32	YDR527W	RBA50	het	Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.195	4.85E-30	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.193	2.23E-29	YNL134C	YNL134C	hom	Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.192	4.28E-29	YDL152W_d	YDL152W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.185	2.87E-27	YDL150W	RPC53	het	RNA polymerase III subunit C53
0.175	2.15E-24	YLR243W	GPN3	het	Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.170	3.29E-23	YMR283C	RIT1	hom	2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.167	2.28E-22	YBR257W	POP4	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.162	4.40E-21	YHR025W	THR1	hom	Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway