YGR086C / PIL1

Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR086C / PIL1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:-3.21|P-value:4.04E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:-3.48|P-value:1.38E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:3.29|P-value:2.70E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:4.07|P-value:9.30E-6 ID:SGTC_269|Compound:exalamide|FD-Score:-3.13|P-value:5.52E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:5.53|P-value:3.12E-9 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.21|P-value:3.64E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.06|P-value:1.14E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:3.46|P-value:1.33E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.85|P-value:2.60E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:3.04|P-value:6.94E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:3.41|P-value:1.63E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:3.05|P-value:6.60E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:4.06|P-value:9.87E-6 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.08|P-value:5.80E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.58|P-value:8.30E-5 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.19|P-value:3.91E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.16|P-value:4.36E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.59|P-value:7.05E-7 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.16|P-value:4.95E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.07|P-value:6.92E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:3.54|P-value:9.91E-5 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.03|P-value:7.98E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.41|P-value:1.63E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.88|P-value:2.25E-5 ID:SGTC_1716|Compound:st033144|FD-Score:3.72|P-value:4.50E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.62|P-value:6.94E-5 ID:SGTC_1853|Compound:st056710|FD-Score:8.41|P-value:5.28E-19 ID:SGTC_2005|Compound:4024620|FD-Score:3.38|P-value:1.85E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.92|P-value:1.84E-5 ID:SGTC_2144|Compound:5628481|FD-Score:-3.80|P-value:3.70E-5 ID:SGTC_2155|Compound:5570423|FD-Score:4.55|P-value:8.79E-7 ID:SGTC_2226|Compound:7298446|FD-Score:3.05|P-value:6.62E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.13|P-value:4.97E-4 ID:SGTC_2368|Compound:9071271|FD-Score:-3.88|P-value:2.55E-5 ID:SGTC_2378|Compound:9074213|FD-Score:-3.58|P-value:9.22E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.60|P-value:7.09E-16 ID:SGTC_2713|Compound:st079149|FD-Score:3.74|P-value:4.14E-5 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.86|P-value:2.76E-5 ID:SGTC_2831|Compound:7999036|FD-Score:-2.97|P-value:9.60E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-3.43|P-value:1.71E-4 ID:SGTC_2875|Compound:9039966|FD-Score:3.05|P-value:6.49E-4 ID:SGTC_2880|Compound:9042983|FD-Score:2.98|P-value:8.41E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.46|P-value:1.33E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.27|P-value:3.48E-6 ID:SGTC_3058|Compound:9093861|FD-Score:3.88|P-value:2.23E-5 ID:SGTC_3128|Compound:9127691|FD-Score:-3.82|P-value:3.32E-5 ID:SGTC_3137|Compound:9097352|FD-Score:-3.89|P-value:2.41E-5 ID:SGTC_3152|Compound:9099253|FD-Score:3.21|P-value:3.67E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.18|P-value:5.58E-6 ID:SGTC_3263|Compound:9137018|FD-Score:-3.18|P-value:4.61E-4 ID:SGTC_3274|Compound:9138298|FD-Score:3.02|P-value:7.36E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:-3.21|P-value:4.04E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:-3.48|P-value:1.38E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:3.29|P-value:2.70E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:4.07|P-value:9.30E-6 ID:SGTC_269|Compound:exalamide|FD-Score:-3.13|P-value:5.52E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:5.53|P-value:3.12E-9 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.21|P-value:3.64E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.06|P-value:1.14E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:3.46|P-value:1.33E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.85|P-value:2.60E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:3.04|P-value:6.94E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:3.41|P-value:1.63E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:3.05|P-value:6.60E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:4.06|P-value:9.87E-6 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.08|P-value:5.80E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.58|P-value:8.30E-5 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.19|P-value:3.91E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.16|P-value:4.36E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.59|P-value:7.05E-7 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.16|P-value:4.95E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.07|P-value:6.92E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:3.54|P-value:9.91E-5 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.03|P-value:7.98E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.41|P-value:1.63E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.88|P-value:2.25E-5 ID:SGTC_1716|Compound:st033144|FD-Score:3.72|P-value:4.50E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.62|P-value:6.94E-5 ID:SGTC_1853|Compound:st056710|FD-Score:8.41|P-value:5.28E-19 ID:SGTC_2005|Compound:4024620|FD-Score:3.38|P-value:1.85E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.92|P-value:1.84E-5 ID:SGTC_2144|Compound:5628481|FD-Score:-3.80|P-value:3.70E-5 ID:SGTC_2155|Compound:5570423|FD-Score:4.55|P-value:8.79E-7 ID:SGTC_2226|Compound:7298446|FD-Score:3.05|P-value:6.62E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.13|P-value:4.97E-4 ID:SGTC_2368|Compound:9071271|FD-Score:-3.88|P-value:2.55E-5 ID:SGTC_2378|Compound:9074213|FD-Score:-3.58|P-value:9.22E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.60|P-value:7.09E-16 ID:SGTC_2713|Compound:st079149|FD-Score:3.74|P-value:4.14E-5 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.86|P-value:2.76E-5 ID:SGTC_2831|Compound:7999036|FD-Score:-2.97|P-value:9.60E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-3.43|P-value:1.71E-4 ID:SGTC_2875|Compound:9039966|FD-Score:3.05|P-value:6.49E-4 ID:SGTC_2880|Compound:9042983|FD-Score:2.98|P-value:8.41E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.46|P-value:1.33E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.27|P-value:3.48E-6 ID:SGTC_3058|Compound:9093861|FD-Score:3.88|P-value:2.23E-5 ID:SGTC_3128|Compound:9127691|FD-Score:-3.82|P-value:3.32E-5 ID:SGTC_3137|Compound:9097352|FD-Score:-3.89|P-value:2.41E-5 ID:SGTC_3152|Compound:9099253|FD-Score:3.21|P-value:3.67E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.18|P-value:5.58E-6 ID:SGTC_3263|Compound:9137018|FD-Score:-3.18|P-value:4.61E-4 ID:SGTC_3274|Compound:9138298|FD-Score:3.02|P-value:7.36E-4

Top fitness defect scores for YGR086C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1853 st056710 8.41 91.2 µM 5.28E-19 amide catabolism 2 2.08E-17
2 SGTC_2688 kanamycin b 7.60 96.7 µM 7.09E-16 calcium & mitochondrial duress 83 1.46E-14
3 SGTC_382 3229-0966 5.53 23.2 µM 3.12E-9 2 1.62E-8
4 SGTC_1245 0418-0076 4.59 17.2 µM 7.05E-7 ERG2 17 2.25E-6
5 SGTC_2155 5570423 4.55 76.0 µM 8.79E-7 ergosterol biosynthesis 7 2.75E-6
6 SGTC_3037 9091026 4.27 49.5 µM 3.48E-6 NEO1-PIK1 106 9.61E-6
7 SGTC_3221 9128209 4.18 49.5 µM 5.58E-6 10 1.48E-5
8 SGTC_206 4546-0025 4.07 72.0 µM 9.30E-6 NEO1 64 2.35E-5
9 SGTC_1021 4476-0760 4.06 85.3 µM 9.87E-6 20 2.49E-5
10 SGTC_2131 toluylene red 3.92 5.3 µM 1.84E-5 60S ribosome export 25 4.38E-5
11 SGTC_3058 9093861 3.88 49.5 µM 2.23E-5 17 5.22E-5
12 SGTC_1715 st032160 3.88 10.2 µM 2.25E-5 45 5.27E-5
13 SGTC_682 0kpi-0099 3.85 17.0 µM 2.60E-5 76 6.01E-5
14 SGTC_2713 st079149 3.74 53.4 µM 4.14E-5 27 9.18E-5
15 SGTC_1716 st033144 3.72 30.6 µM 4.50E-5 27 9.89E-5
16 SGTC_1787 fluconazole 3.62 80.0 µM 6.94E-5 iron homeostasis 37 1.47E-4
17 SGTC_1155 4088-0394 3.58 48.2 µM 8.30E-5 44 1.73E-4
18 SGTC_1362 1611-0397 3.54 101.0 µM 9.91E-5 31 2.03E-4
19 SGTC_517 1365-0151 3.46 113.0 µM 1.33E-4 46 2.66E-4
20 SGTC_2967 9089538 3.46 49.9 µM 1.33E-4 52 2.67E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 1.24E-24 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.174 3.67E-24 YOR008C-A_p YOR008C-A_p hom Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.158 4.55E-20 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.142 1.54E-16 YIL002C INP51 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
0.130 4.44E-14 YBR287W_p YBR287W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.126 3.13E-13 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.125 3.96E-13 YLR360W VPS38 hom Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated
0.123 9.68E-13 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.119 4.16E-12 YOR216C RUD3 hom Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.118 6.08E-12 YLR443W ECM7 hom Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
0.117 1.14E-11 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.117 1.23E-11 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.116 1.39E-11 YDL052C SLC1 hom 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
0.116 1.63E-11 YBR175W SWD3 hom Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
0.115 2.80E-11 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion