YGR089W / NNF2

Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR089W / NNF2

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:-3.59|P-value:1.97E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:4.98|P-value:1.93E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.48|P-value:1.25E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:5.19|P-value:6.27E-8 ID:SGTC_86|Compound:0417-1630|FD-Score:3.47|P-value:1.92E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:11.00|P-value:7.64E-29 ID:SGTC_142|Compound:0335-0849|FD-Score:3.41|P-value:2.36E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.54|P-value:2.40E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-3.62|P-value:1.82E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.47|P-value:1.92E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.18|P-value:5.42E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:12.10|P-value:6.45E-35 ID:SGTC_165|Compound:k038-0023|FD-Score:3.76|P-value:5.92E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.76|P-value:1.03E-4 ID:SGTC_192|Compound:3455-0609|FD-Score:3.31|P-value:3.42E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:-3.47|P-value:3.13E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-4.09|P-value:2.64E-5 ID:SGTC_259|Compound:usnic acid|FD-Score:3.12|P-value:6.82E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:3.87|P-value:3.87E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:5.50|P-value:1.12E-8 ID:SGTC_299|Compound:1273-0059|FD-Score:3.65|P-value:9.30E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.07|P-value:2.91E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.15|P-value:9.64E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.20|P-value:1.63E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:-3.55|P-value:2.31E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:6.63|P-value:8.30E-12 ID:SGTC_320|Compound:4092-0846|FD-Score:-3.30|P-value:5.81E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:5.62|P-value:5.47E-9 ID:SGTC_424|Compound:splitomicin|FD-Score:3.35|P-value:2.93E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.48|P-value:3.02E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.36|P-value:4.62E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.32|P-value:3.05E-8 ID:SGTC_552|Compound:k242-0005|FD-Score:3.21|P-value:4.87E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.04|P-value:9.00E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.69|P-value:7.84E-5 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.27|P-value:6.36E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:5.51|P-value:1.07E-8 ID:SGTC_730|Compound:1436-0052|FD-Score:3.28|P-value:3.81E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:4.37|P-value:4.04E-6 ID:SGTC_759|Compound:3486-0358|FD-Score:-3.20|P-value:8.19E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.15|P-value:1.13E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.15|P-value:6.12E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.59|P-value:1.20E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.22|P-value:4.69E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.14|P-value:6.37E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.77|P-value:5.68E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:3.94|P-value:2.84E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:4.91|P-value:2.83E-7 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.43|P-value:3.65E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.45|P-value:2.05E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:-3.62|P-value:1.77E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:4.39|P-value:3.69E-6 ID:SGTC_1233|Compound:0325-0408|FD-Score:-3.46|P-value:3.29E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.12|P-value:6.75E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.08|P-value:1.56E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.47|P-value:3.12E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.10|P-value:7.31E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.58|P-value:1.25E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-4.14|P-value:2.15E-5 ID:SGTC_1721|Compound:st082152|FD-Score:3.84|P-value:4.32E-5 ID:SGTC_1727|Compound:st034309|FD-Score:3.14|P-value:6.33E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.78|P-value:9.61E-5 ID:SGTC_1996|Compound:st072960|FD-Score:-3.31|P-value:5.66E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.73|P-value:6.91E-5 ID:SGTC_1999|Compound:st074831|FD-Score:3.94|P-value:2.78E-5 ID:SGTC_2036|Compound:5160441|FD-Score:-3.44|P-value:3.54E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.04|P-value:9.08E-4 ID:SGTC_2092|Compound:5303694|FD-Score:-5.55|P-value:1.80E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.41|P-value:3.87E-4 ID:SGTC_2325|Compound:6158266|FD-Score:3.53|P-value:1.52E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.25|P-value:6.95E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.65|P-value:9.24E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.47|P-value:1.86E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.60|P-value:1.12E-4 ID:SGTC_2704|Compound:st078022|FD-Score:3.66|P-value:9.15E-5 ID:SGTC_2907|Compound:7721985|FD-Score:-3.96|P-value:4.59E-5 ID:SGTC_2973|Compound:9094665|FD-Score:3.18|P-value:5.43E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.29|P-value:3.65E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:-3.59|P-value:1.97E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:4.98|P-value:1.93E-7 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.48|P-value:1.25E-8 ID:SGTC_64|Compound:000l-0018|FD-Score:5.19|P-value:6.27E-8 ID:SGTC_86|Compound:0417-1630|FD-Score:3.47|P-value:1.92E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:11.00|P-value:7.64E-29 ID:SGTC_142|Compound:0335-0849|FD-Score:3.41|P-value:2.36E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.54|P-value:2.40E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-3.62|P-value:1.82E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.47|P-value:1.92E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.18|P-value:5.42E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:12.10|P-value:6.45E-35 ID:SGTC_165|Compound:k038-0023|FD-Score:3.76|P-value:5.92E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.76|P-value:1.03E-4 ID:SGTC_192|Compound:3455-0609|FD-Score:3.31|P-value:3.42E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:-3.47|P-value:3.13E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-4.09|P-value:2.64E-5 ID:SGTC_259|Compound:usnic acid|FD-Score:3.12|P-value:6.82E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:3.87|P-value:3.87E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:5.50|P-value:1.12E-8 ID:SGTC_299|Compound:1273-0059|FD-Score:3.65|P-value:9.30E-5 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.07|P-value:2.91E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.15|P-value:9.64E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.20|P-value:1.63E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:-3.55|P-value:2.31E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:6.63|P-value:8.30E-12 ID:SGTC_320|Compound:4092-0846|FD-Score:-3.30|P-value:5.81E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:5.62|P-value:5.47E-9 ID:SGTC_424|Compound:splitomicin|FD-Score:3.35|P-value:2.93E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.48|P-value:3.02E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.36|P-value:4.62E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.32|P-value:3.05E-8 ID:SGTC_552|Compound:k242-0005|FD-Score:3.21|P-value:4.87E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.04|P-value:9.00E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.69|P-value:7.84E-5 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.27|P-value:6.36E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:5.51|P-value:1.07E-8 ID:SGTC_730|Compound:1436-0052|FD-Score:3.28|P-value:3.81E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:4.37|P-value:4.04E-6 ID:SGTC_759|Compound:3486-0358|FD-Score:-3.20|P-value:8.19E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:4.15|P-value:1.13E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.15|P-value:6.12E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.59|P-value:1.20E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.22|P-value:4.69E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:3.14|P-value:6.37E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.77|P-value:5.68E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:3.94|P-value:2.84E-5 ID:SGTC_994|Compound:3910-0338|FD-Score:4.91|P-value:2.83E-7 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.43|P-value:3.65E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.45|P-value:2.05E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:-3.62|P-value:1.77E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:4.39|P-value:3.69E-6 ID:SGTC_1233|Compound:0325-0408|FD-Score:-3.46|P-value:3.29E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.12|P-value:6.75E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.08|P-value:1.56E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.47|P-value:3.12E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.10|P-value:7.31E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.58|P-value:1.25E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:-4.14|P-value:2.15E-5 ID:SGTC_1721|Compound:st082152|FD-Score:3.84|P-value:4.32E-5 ID:SGTC_1727|Compound:st034309|FD-Score:3.14|P-value:6.33E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.78|P-value:9.61E-5 ID:SGTC_1996|Compound:st072960|FD-Score:-3.31|P-value:5.66E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.73|P-value:6.91E-5 ID:SGTC_1999|Compound:st074831|FD-Score:3.94|P-value:2.78E-5 ID:SGTC_2036|Compound:5160441|FD-Score:-3.44|P-value:3.54E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.04|P-value:9.08E-4 ID:SGTC_2092|Compound:5303694|FD-Score:-5.55|P-value:1.80E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.41|P-value:3.87E-4 ID:SGTC_2325|Compound:6158266|FD-Score:3.53|P-value:1.52E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.25|P-value:6.95E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.65|P-value:9.24E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.47|P-value:1.86E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.60|P-value:1.12E-4 ID:SGTC_2704|Compound:st078022|FD-Score:3.66|P-value:9.15E-5 ID:SGTC_2907|Compound:7721985|FD-Score:-3.96|P-value:4.59E-5 ID:SGTC_2973|Compound:9094665|FD-Score:3.18|P-value:5.43E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.29|P-value:3.65E-4

Top fitness defect scores for YGR089W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_158 k038-0087 12.10 26.4 µM 6.45E-35 ubiquinone biosynthesis & proteasome 19 3.07E-34
2 SGTC_103 0844-0104 11.00 51.8 µM 7.64E-29 ERAD & cell cycle 27 2.94E-28
3 SGTC_315 k915-0449 6.63 187.0 µM 8.30E-12 15 1.65E-11
4 SGTC_373 0844-0104 5.62 25.9 µM 5.47E-9 ERAD & cell cycle 23 9.54E-9
5 SGTC_676 falcarinol 5.51 140.0 µM 1.07E-8 21 1.84E-8
6 SGTC_294 k038-0071 5.50 157.0 µM 1.12E-8 ubiquinone biosynthesis & proteasome 24 1.93E-8
7 SGTC_28 pentachloropyridine N-oxide 5.48 1.6 µM 1.25E-8 TSC3-RPN4 24 2.15E-8
8 SGTC_513 4245-1575 5.32 41.7 µM 3.05E-8 unfolded protein response 64 5.14E-8
9 SGTC_64 000l-0018 5.19 272.1 µM 6.27E-8 ubiquinone biosynthesis & proteasome 103 1.04E-7
10 SGTC_16 erodoxin 4.98 22.8 µM 1.93E-7 unfolded protein response 39 3.12E-7
11 SGTC_994 3910-0338 4.91 138.0 µM 2.83E-7 calcium & mitochondrial duress 23 4.55E-7
12 SGTC_1224 0823-0416 4.39 294.0 µM 3.69E-6 1 5.59E-6
13 SGTC_742 0kpi-0133 4.37 140.0 µM 4.04E-6 ERG2 16 6.11E-6
14 SGTC_828 2074-0507 4.15 139.0 µM 1.13E-5 RPP1 & pyrimidine depletion 21 1.66E-5
15 SGTC_1450 4112-4260 4.08 37.6 µM 1.56E-5 calcium & mitochondrial duress 24 2.28E-5
16 SGTC_1999 st074831 3.94 12.5 µM 2.78E-5 plasma membrane duress 32 4.02E-5
17 SGTC_984 1492-0255 3.94 2.9 µM 2.84E-5 SWF1 & branched chain AA biosynthesis 29 4.10E-5
18 SGTC_287 k060-0039 3.87 29.6 µM 3.87E-5 12 5.54E-5
19 SGTC_1721 st082152 3.84 9.3 µM 4.32E-5 60S ribosome export 23 6.17E-5
20 SGTC_878 0828-0289 3.77 13.7 µM 5.68E-5 54 8.06E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.347 1.72E-95 YEL043W YEL043W hom Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
0.343 3.61E-93 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.301 2.00E-71 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.288 2.53E-65 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.270 6.46E-57 YNL149C PGA2 het Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
0.268 3.95E-56 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.265 3.63E-55 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.260 6.60E-53 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.255 4.19E-51 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.248 2.60E-48 YFR007W YFH7 hom Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases
0.236 9.08E-44 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.235 2.36E-43 YML034W SRC1 hom Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
0.235 2.22E-43 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.233 1.07E-42 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.228 8.58E-41 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication