YGR096W / TPC1

Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR096W / TPC1

Click on Significant Values for Screen Details ID:SGTC_358|Compound:0075-0058|FD-Score:3.71|P-value:1.49E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.37|P-value:5.36E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:3.24|P-value:8.32E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:-3.11|P-value:6.71E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.63|P-value:2.01E-4 ID:SGTC_1819|Compound:st053448|FD-Score:4.14|P-value:2.61E-5 ID:SGTC_2025|Compound:5147754|FD-Score:-4.18|P-value:9.56E-6 ID:SGTC_2027|Compound:5150920|FD-Score:-3.11|P-value:6.81E-4 ID:SGTC_2061|Compound:5245884|FD-Score:-3.93|P-value:2.92E-5 ID:SGTC_2394|Compound:5221648|FD-Score:3.42|P-value:4.37E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.72|P-value:1.44E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-4.21|P-value:8.37E-6 ID:SGTC_2708|Compound:st078559|FD-Score:-3.17|P-value:5.41E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:5.07|P-value:3.33E-7 ID:SGTC_2956|Compound:9076196|FD-Score:3.30|P-value:6.65E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.71|P-value:1.49E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.37|P-value:5.36E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:3.24|P-value:8.32E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:-3.11|P-value:6.71E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.63|P-value:2.01E-4 ID:SGTC_1819|Compound:st053448|FD-Score:4.14|P-value:2.61E-5 ID:SGTC_2025|Compound:5147754|FD-Score:-4.18|P-value:9.56E-6 ID:SGTC_2027|Compound:5150920|FD-Score:-3.11|P-value:6.81E-4 ID:SGTC_2061|Compound:5245884|FD-Score:-3.93|P-value:2.92E-5 ID:SGTC_2394|Compound:5221648|FD-Score:3.42|P-value:4.37E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.72|P-value:1.44E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-4.21|P-value:8.37E-6 ID:SGTC_2708|Compound:st078559|FD-Score:-3.17|P-value:5.41E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:5.07|P-value:3.33E-7 ID:SGTC_2956|Compound:9076196|FD-Score:3.30|P-value:6.65E-4

Top fitness defect scores for YGR096W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2762 leflunomide 5.07 42.1 µM 3.33E-7 10 2.02E-7
2 SGTC_1819 st053448 4.14 58.6 µM 2.61E-5 amide catabolism 14 1.73E-5
3 SGTC_2404 5161160 3.72 200.0 µM 1.44E-4 26 9.89E-5
4 SGTC_358 0075-0058 3.71 9.4 µM 1.49E-4 74 1.03E-4
5 SGTC_1226 3909-7979 3.63 35.4 µM 2.01E-4 52 1.40E-4
6 SGTC_2394 5221648 3.42 200.0 µM 4.37E-4 42 3.09E-4
7 SGTC_508 roscovitine 3.37 141.0 µM 5.36E-4 15 3.81E-4
8 SGTC_2956 9076196 3.30 2.5 µM 6.65E-4 48 4.76E-4
9 SGTC_521 r062-0005 3.24 13.8 µM 8.32E-4 38 5.98E-4
10 SGTC_1274 0865-0114 3.17 173.0 µM 0.00106 45 7.67E-4
11 SGTC_6 0844-0013 3.06 19.8 µM 0.00153 203 0.00112
12 SGTC_417 st075190 2.94 100.0 µM 0.00221 53 0.00163
13 SGTC_1412 3909-8563 2.94 178.0 µM 0.00222 50 0.00164
14 SGTC_1147 3809-1045 2.87 12.3 µM 0.00273 85 0.00203
15 SGTC_2930 9013230 2.87 14.9 µM 0.00275 heme biosynthesis & mitochondrial translocase 105 0.00204
16 SGTC_1159 4092-0538 2.84 61.2 µM 0.00298 82 0.00222
17 SGTC_403 arphamenine a 2.83 50.0 µM 0.00308 60S ribosome export 72 0.00230
18 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 2.83 3.7 µM 0.00309 240 0.00230
19 SGTC_298 1391-0729 2.82 10.0 µM 0.00320 calcium & mitochondrial duress 52 0.00239
20 SGTC_3098 9119039 2.79 49.5 µM 0.00352 Golgi 92 0.00263

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.220 7.13E-38 YLR237W THI7 hom Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
0.182 2.00E-26 YPL092W SSU1 hom Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein
0.160 1.55E-20 YDL080C THI3 hom Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis
0.147 1.01E-17 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.147 1.44E-17 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.143 1.04E-16 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.136 3.24E-15 YPL249C GYP5 hom GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.129 6.81E-14 YMR177W MMT1 hom Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p
0.128 1.31E-13 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.125 3.75E-13 YKL161C KDX1 hom Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
0.124 5.08E-13 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
0.124 5.19E-13 YLR328W NMA1 hom Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
0.124 5.32E-13 YFR012W DCV1 hom Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication
0.122 1.19E-12 YOR225W_d YOR225W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.121 2.40E-12 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex