YGR098C / ESP1

Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR098C / ESP1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.76|P-value:6.32E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-8.35|P-value:2.57E-13 ID:SGTC_141|Compound:0443-0274|FD-Score:-7.28|P-value:1.46E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:4.06|P-value:2.44E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:5.64|P-value:6.21E-7 ID:SGTC_257|Compound:daunorubicin|FD-Score:5.98|P-value:1.33E-7 ID:SGTC_263|Compound:alizarin|FD-Score:3.81|P-value:5.33E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:3.88|P-value:4.31E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:4.09|P-value:2.25E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:6.44|P-value:1.48E-8 ID:SGTC_309|Compound:k408-0051|FD-Score:-7.04|P-value:5.68E-10 ID:SGTC_312|Compound:4092-0653|FD-Score:4.20|P-value:1.57E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:3.98|P-value:3.16E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.95|P-value:9.24E-10 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-5.66|P-value:4.80E-7 ID:SGTC_525|Compound:k781-2675|FD-Score:4.26|P-value:1.25E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.81|P-value:1.81E-5 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.63|P-value:8.06E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:3.91|P-value:3.88E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:6.04|P-value:1.02E-7 ID:SGTC_640|Compound:3381-0960|FD-Score:-5.06|P-value:5.94E-6 ID:SGTC_642|Compound:0335-0876|FD-Score:-4.01|P-value:2.55E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:3.65|P-value:8.55E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:4.36|P-value:8.97E-5 ID:SGTC_848|Compound:0368-0089|FD-Score:3.69|P-value:7.77E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:4.82|P-value:1.72E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:5.04|P-value:7.52E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-3.72|P-value:6.27E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:4.70|P-value:2.68E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:5.22|P-value:3.64E-6 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.98|P-value:3.16E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.58|P-value:9.47E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.69|P-value:6.91E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.09|P-value:2.19E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:-4.88|P-value:1.19E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:-3.89|P-value:3.75E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.09|P-value:2.23E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.85|P-value:4.69E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:4.05|P-value:2.55E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:13.30|P-value:1.12E-30 ID:SGTC_1633|Compound:st005664|FD-Score:-3.63|P-value:8.07E-4 ID:SGTC_1637|Compound:st007924|FD-Score:4.63|P-value:3.53E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-4.64|P-value:2.95E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.61|P-value:3.24E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.62|P-value:8.44E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.72|P-value:6.30E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.20|P-value:3.91E-6 ID:SGTC_1898|Compound:st060199|FD-Score:-3.60|P-value:8.85E-4 ID:SGTC_1912|Compound:st060209|FD-Score:5.09|P-value:6.08E-6 ID:SGTC_1914|Compound:st062367|FD-Score:-4.29|P-value:9.97E-5 ID:SGTC_2008|Compound:4012253|FD-Score:6.37|P-value:2.11E-8 ID:SGTC_2009|Compound:4012297|FD-Score:-3.70|P-value:6.70E-4 ID:SGTC_2010|Compound:4012422|FD-Score:6.66|P-value:5.03E-9 ID:SGTC_2021|Compound:4019055|FD-Score:8.32|P-value:3.93E-13 ID:SGTC_2065|Compound:5221596|FD-Score:-3.60|P-value:9.05E-4 ID:SGTC_2073|Compound:5212005|FD-Score:-3.98|P-value:2.80E-4 ID:SGTC_2094|Compound:5332512|FD-Score:5.20|P-value:3.89E-6 ID:SGTC_2137|Compound:5261020|FD-Score:3.67|P-value:8.06E-4 ID:SGTC_2238|Compound:6671620|FD-Score:4.74|P-value:2.30E-5 ID:SGTC_2360|Compound:9040895|FD-Score:3.62|P-value:9.40E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.00|P-value:2.95E-4 ID:SGTC_2380|Compound:9035439|FD-Score:4.07|P-value:2.39E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:-3.70|P-value:6.58E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:9.71|P-value:3.11E-17 ID:SGTC_2676|Compound:harmalol|FD-Score:3.99|P-value:3.02E-4 ID:SGTC_2702|Compound:st077768|FD-Score:4.37|P-value:8.57E-5 ID:SGTC_2717|Compound:st081029|FD-Score:3.79|P-value:5.62E-4 ID:SGTC_2784|Compound:7567673|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_2896|Compound:9052656|FD-Score:7.64|P-value:2.47E-11 ID:SGTC_2914|Compound:7968218|FD-Score:3.91|P-value:3.96E-4 ID:SGTC_2985|Compound:9022375|FD-Score:4.10|P-value:2.15E-4 ID:SGTC_2991|Compound:9074669|FD-Score:11.50|P-value:1.33E-23 ID:SGTC_2994|Compound:9053520|FD-Score:4.77|P-value:2.07E-5 ID:SGTC_2999|Compound:9069765|FD-Score:3.86|P-value:4.59E-4 ID:SGTC_3142|Compound:9086498|FD-Score:12.70|P-value:3.90E-28 ID:SGTC_3211|Compound:9128781|FD-Score:5.85|P-value:2.46E-7 ID:SGTC_3314|Compound:9135860|FD-Score:4.15|P-value:1.82E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:3.76|P-value:6.32E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-8.35|P-value:2.57E-13 ID:SGTC_141|Compound:0443-0274|FD-Score:-7.28|P-value:1.46E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:4.06|P-value:2.44E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:5.64|P-value:6.21E-7 ID:SGTC_257|Compound:daunorubicin|FD-Score:5.98|P-value:1.33E-7 ID:SGTC_263|Compound:alizarin|FD-Score:3.81|P-value:5.33E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:3.88|P-value:4.31E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:4.09|P-value:2.25E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:6.44|P-value:1.48E-8 ID:SGTC_309|Compound:k408-0051|FD-Score:-7.04|P-value:5.68E-10 ID:SGTC_312|Compound:4092-0653|FD-Score:4.20|P-value:1.57E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:3.98|P-value:3.16E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.95|P-value:9.24E-10 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-5.66|P-value:4.80E-7 ID:SGTC_525|Compound:k781-2675|FD-Score:4.26|P-value:1.25E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.81|P-value:1.81E-5 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.63|P-value:8.06E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:3.91|P-value:3.88E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:6.04|P-value:1.02E-7 ID:SGTC_640|Compound:3381-0960|FD-Score:-5.06|P-value:5.94E-6 ID:SGTC_642|Compound:0335-0876|FD-Score:-4.01|P-value:2.55E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:3.65|P-value:8.55E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:4.36|P-value:8.97E-5 ID:SGTC_848|Compound:0368-0089|FD-Score:3.69|P-value:7.77E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:4.82|P-value:1.72E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:5.04|P-value:7.52E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-3.72|P-value:6.27E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:4.70|P-value:2.68E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:5.22|P-value:3.64E-6 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.98|P-value:3.16E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.58|P-value:9.47E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.69|P-value:6.91E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.09|P-value:2.19E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:-4.88|P-value:1.19E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:-3.89|P-value:3.75E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.09|P-value:2.23E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.85|P-value:4.69E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:4.05|P-value:2.55E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:13.30|P-value:1.12E-30 ID:SGTC_1633|Compound:st005664|FD-Score:-3.63|P-value:8.07E-4 ID:SGTC_1637|Compound:st007924|FD-Score:4.63|P-value:3.53E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-4.64|P-value:2.95E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.61|P-value:3.24E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-3.62|P-value:8.44E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.72|P-value:6.30E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.20|P-value:3.91E-6 ID:SGTC_1898|Compound:st060199|FD-Score:-3.60|P-value:8.85E-4 ID:SGTC_1912|Compound:st060209|FD-Score:5.09|P-value:6.08E-6 ID:SGTC_1914|Compound:st062367|FD-Score:-4.29|P-value:9.97E-5 ID:SGTC_2008|Compound:4012253|FD-Score:6.37|P-value:2.11E-8 ID:SGTC_2009|Compound:4012297|FD-Score:-3.70|P-value:6.70E-4 ID:SGTC_2010|Compound:4012422|FD-Score:6.66|P-value:5.03E-9 ID:SGTC_2021|Compound:4019055|FD-Score:8.32|P-value:3.93E-13 ID:SGTC_2065|Compound:5221596|FD-Score:-3.60|P-value:9.05E-4 ID:SGTC_2073|Compound:5212005|FD-Score:-3.98|P-value:2.80E-4 ID:SGTC_2094|Compound:5332512|FD-Score:5.20|P-value:3.89E-6 ID:SGTC_2137|Compound:5261020|FD-Score:3.67|P-value:8.06E-4 ID:SGTC_2238|Compound:6671620|FD-Score:4.74|P-value:2.30E-5 ID:SGTC_2360|Compound:9040895|FD-Score:3.62|P-value:9.40E-4 ID:SGTC_2365|Compound:9042475|FD-Score:4.00|P-value:2.95E-4 ID:SGTC_2380|Compound:9035439|FD-Score:4.07|P-value:2.39E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:-3.70|P-value:6.58E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:9.71|P-value:3.11E-17 ID:SGTC_2676|Compound:harmalol|FD-Score:3.99|P-value:3.02E-4 ID:SGTC_2702|Compound:st077768|FD-Score:4.37|P-value:8.57E-5 ID:SGTC_2717|Compound:st081029|FD-Score:3.79|P-value:5.62E-4 ID:SGTC_2784|Compound:7567673|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_2896|Compound:9052656|FD-Score:7.64|P-value:2.47E-11 ID:SGTC_2914|Compound:7968218|FD-Score:3.91|P-value:3.96E-4 ID:SGTC_2985|Compound:9022375|FD-Score:4.10|P-value:2.15E-4 ID:SGTC_2991|Compound:9074669|FD-Score:11.50|P-value:1.33E-23 ID:SGTC_2994|Compound:9053520|FD-Score:4.77|P-value:2.07E-5 ID:SGTC_2999|Compound:9069765|FD-Score:3.86|P-value:4.59E-4 ID:SGTC_3142|Compound:9086498|FD-Score:12.70|P-value:3.90E-28 ID:SGTC_3211|Compound:9128781|FD-Score:5.85|P-value:2.46E-7 ID:SGTC_3314|Compound:9135860|FD-Score:4.15|P-value:1.82E-4

Top fitness defect scores for YGR098C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1597 7-benzyloxygramine 13.30 47.5 µM 1.12E-30 calcium & mitochondrial duress 12 1.26E-40 0.82
2 SGTC_3142 9086498 12.70 49.5 µM 3.90E-28 iron homeostasis 5 3.26E-37 1.08
3 SGTC_2991 9074669 11.50 71.4 µM 1.33E-23 azole & statin 3 3.96E-31 3.67
4 SGTC_2662 daunorubicin 9.71 22.4 µM 3.11E-17 anthracycline transcription coupled DNA repair 3 1.38E-22 0.19
5 SGTC_2021 4019055 8.32 173.0 µM 3.93E-13 endomembrane recycling 10 4.35E-17 0.53
6 SGTC_2896 9052656 7.64 71.4 µM 2.47E-11 endomembrane recycling 6 1.12E-14 0.52
7 SGTC_2010 4012422 6.66 159.0 µM 5.03E-9 2 1.37E-11 0.81
8 SGTC_308 4092-1169 6.44 104.0 µM 1.48E-8 plasma membrane duress 2 5.80E-11 1.53
9 SGTC_2008 4012253 6.37 172.0 µM 2.11E-8 1 9.35E-11 0.81
10 SGTC_609 0986-0026 6.04 86.3 µM 1.02E-7 calcium & mitochondrial duress 7 7.65E-10 0.08
11 SGTC_257 daunorubicin 5.98 18.5 µM 1.33E-7 anthracycline transcription coupled DNA repair 8 1.09E-9 1.07
12 SGTC_3211 9128781 5.85 49.5 µM 2.46E-7 5 2.49E-9 0.13
13 SGTC_227 fluconazole 5.64 33.5 µM 6.21E-7 azole & statin 32 8.56E-9 0.24
14 SGTC_1035 k081-0041 5.22 55.2 µM 3.64E-6 Golgi 4 9.06E-8 1.09
15 SGTC_2094 5332512 5.20 191.7 µM 3.89E-6 1 9.91E-8 0.75
16 SGTC_1844 rapamycin 5.20 4.0 nM 3.91E-6 30 9.98E-8 0.09
17 SGTC_1912 st060209 5.09 90.8 µM 6.08E-6 5 1.80E-7 0.40
18 SGTC_911 3456-4043 5.04 236.0 µM 7.52E-6 mitochondrial processes 4 2.38E-7 0.47
19 SGTC_873 0591-4978 4.82 488.0 µM 1.72E-5 2 7.17E-7 0.09
20 SGTC_576 1073-0113 4.81 22.7 µM 1.81E-5 redox potentiating 30 7.67E-7 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.185 3.53E-27 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.182 3.05E-26 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.177 6.15E-25 YFR051C RET2 het Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.176 1.15E-24 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.166 4.50E-22 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.163 2.24E-21 YGL113W SLD3 het Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
0.162 3.44E-21 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.149 3.91E-18 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.146 1.91E-17 YIL143C SSL2 het Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
0.146 2.38E-17 YDL196W_d YDL196W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
0.143 9.00E-17 YER133W GLC7 het Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
0.140 4.00E-16 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.140 4.96E-16 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.139 5.88E-16 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.131 3.17E-14 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress