YGR104C / SRB5

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR104C / SRB5

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:3.18|P-value:9.59E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.48|P-value:2.49E-8 ID:SGTC_91|Compound:0417-1751|FD-Score:4.07|P-value:3.36E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:3.56|P-value:2.48E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.64|P-value:1.83E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.74|P-value:1.16E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:3.95|P-value:5.62E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:4.05|P-value:3.74E-5 ID:SGTC_279|Compound:1273-0055|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:4.94|P-value:6.50E-7 ID:SGTC_620|Compound:0109-0052|FD-Score:3.29|P-value:6.51E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.65|P-value:2.64E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:3.33|P-value:5.80E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:4.71|P-value:1.94E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:6.79|P-value:1.23E-11 ID:SGTC_801|Compound:0866-0028|FD-Score:4.24|P-value:1.61E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:5.91|P-value:3.28E-9 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.56|P-value:1.88E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-3.22|P-value:6.42E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:4.02|P-value:4.13E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:7.47|P-value:1.08E-13 ID:SGTC_977|Compound:1348-1378|FD-Score:3.55|P-value:2.63E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.34|P-value:5.48E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.11|P-value:9.22E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.83|P-value:1.07E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:3.78|P-value:1.09E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.91|P-value:6.62E-5 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.74|P-value:1.26E-4 ID:SGTC_1627|Compound:st003713|FD-Score:5.99|P-value:2.02E-9 ID:SGTC_1628|Compound:st004252|FD-Score:-3.50|P-value:2.39E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-4.77|P-value:1.04E-6 ID:SGTC_1636|Compound:st007707|FD-Score:4.85|P-value:1.01E-6 ID:SGTC_1637|Compound:st007924|FD-Score:-3.45|P-value:2.88E-4 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.49|P-value:3.29E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.83|P-value:8.78E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.56|P-value:1.93E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.76|P-value:8.83E-5 ID:SGTC_1741|Compound:st038134|FD-Score:3.76|P-value:1.19E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.41|P-value:3.24E-4 ID:SGTC_1834|Compound:st055981|FD-Score:3.64|P-value:1.89E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.64|P-value:1.85E-4 ID:SGTC_1870|Compound:st057514|FD-Score:5.32|P-value:8.83E-8 ID:SGTC_1876|Compound:myriocin|FD-Score:7.21|P-value:6.72E-13 ID:SGTC_1980|Compound:st071811|FD-Score:3.53|P-value:2.82E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.66|P-value:1.74E-4 ID:SGTC_2087|Compound:5276107|FD-Score:4.44|P-value:6.71E-6 ID:SGTC_2089|Compound:5281248|FD-Score:-3.33|P-value:4.40E-4 ID:SGTC_2092|Compound:5303694|FD-Score:4.34|P-value:1.06E-5 ID:SGTC_2136|Compound:5328288|FD-Score:3.44|P-value:3.88E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.19|P-value:9.33E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.94|P-value:5.82E-5 ID:SGTC_2266|Compound:7929956|FD-Score:4.17|P-value:2.23E-5 ID:SGTC_2290|Compound:7904846|FD-Score:3.54|P-value:2.72E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.22|P-value:8.42E-4 ID:SGTC_2414|Compound:st077232|FD-Score:3.38|P-value:4.83E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.71|P-value:1.05E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.37|P-value:3.80E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-4.47|P-value:4.27E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:5.67|P-value:1.30E-8 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.28|P-value:6.73E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.40|P-value:5.87E-6 ID:SGTC_2741|Compound:miltefosine|FD-Score:5.74|P-value:8.62E-9 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.51|P-value:2.97E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.79|P-value:1.06E-4 ID:SGTC_3217|Compound:9129673|FD-Score:-3.45|P-value:2.83E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-3.97|P-value:3.70E-5 ID:SGTC_3281|Compound:9140613|FD-Score:4.51|P-value:4.90E-6 ID:SGTC_16|Compound:erodoxin|FD-Score:3.18|P-value:9.59E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.48|P-value:2.49E-8 ID:SGTC_91|Compound:0417-1751|FD-Score:4.07|P-value:3.36E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:3.56|P-value:2.48E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.64|P-value:1.83E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.74|P-value:1.16E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:3.95|P-value:5.62E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:4.05|P-value:3.74E-5 ID:SGTC_279|Compound:1273-0055|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:4.94|P-value:6.50E-7 ID:SGTC_620|Compound:0109-0052|FD-Score:3.29|P-value:6.51E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.65|P-value:2.64E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:3.33|P-value:5.80E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:4.71|P-value:1.94E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:6.79|P-value:1.23E-11 ID:SGTC_801|Compound:0866-0028|FD-Score:4.24|P-value:1.61E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:5.91|P-value:3.28E-9 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.56|P-value:1.88E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-3.22|P-value:6.42E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:4.02|P-value:4.13E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:7.47|P-value:1.08E-13 ID:SGTC_977|Compound:1348-1378|FD-Score:3.55|P-value:2.63E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.34|P-value:5.48E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.11|P-value:9.22E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.83|P-value:1.07E-6 ID:SGTC_1078|Compound:idebenone|FD-Score:3.78|P-value:1.09E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.91|P-value:6.62E-5 ID:SGTC_1417|Compound:3966-0327|FD-Score:3.74|P-value:1.26E-4 ID:SGTC_1627|Compound:st003713|FD-Score:5.99|P-value:2.02E-9 ID:SGTC_1628|Compound:st004252|FD-Score:-3.50|P-value:2.39E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-4.77|P-value:1.04E-6 ID:SGTC_1636|Compound:st007707|FD-Score:4.85|P-value:1.01E-6 ID:SGTC_1637|Compound:st007924|FD-Score:-3.45|P-value:2.88E-4 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.49|P-value:3.29E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.83|P-value:8.78E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.56|P-value:1.93E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.76|P-value:8.83E-5 ID:SGTC_1741|Compound:st038134|FD-Score:3.76|P-value:1.19E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-3.41|P-value:3.24E-4 ID:SGTC_1834|Compound:st055981|FD-Score:3.64|P-value:1.89E-4 ID:SGTC_1848|Compound:st056003|FD-Score:3.64|P-value:1.85E-4 ID:SGTC_1870|Compound:st057514|FD-Score:5.32|P-value:8.83E-8 ID:SGTC_1876|Compound:myriocin|FD-Score:7.21|P-value:6.72E-13 ID:SGTC_1980|Compound:st071811|FD-Score:3.53|P-value:2.82E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.66|P-value:1.74E-4 ID:SGTC_2087|Compound:5276107|FD-Score:4.44|P-value:6.71E-6 ID:SGTC_2089|Compound:5281248|FD-Score:-3.33|P-value:4.40E-4 ID:SGTC_2092|Compound:5303694|FD-Score:4.34|P-value:1.06E-5 ID:SGTC_2136|Compound:5328288|FD-Score:3.44|P-value:3.88E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.19|P-value:9.33E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.94|P-value:5.82E-5 ID:SGTC_2266|Compound:7929956|FD-Score:4.17|P-value:2.23E-5 ID:SGTC_2290|Compound:7904846|FD-Score:3.54|P-value:2.72E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.22|P-value:8.42E-4 ID:SGTC_2414|Compound:st077232|FD-Score:3.38|P-value:4.83E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.71|P-value:1.05E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.37|P-value:3.80E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-4.47|P-value:4.27E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:5.67|P-value:1.30E-8 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.28|P-value:6.73E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.40|P-value:5.87E-6 ID:SGTC_2741|Compound:miltefosine|FD-Score:5.74|P-value:8.62E-9 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.51|P-value:2.97E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.79|P-value:1.06E-4 ID:SGTC_3217|Compound:9129673|FD-Score:-3.45|P-value:2.83E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-3.97|P-value:3.70E-5 ID:SGTC_3281|Compound:9140613|FD-Score:4.51|P-value:4.90E-6

Top fitness defect scores for YGR104C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_939 1165-0479 7.47 45.3 µM 1.08E-13 2 4.15E-14
2 SGTC_1876 myriocin 7.21 605.0 nM 6.72E-13 9 2.73E-13
3 SGTC_800 3448-7362 6.79 155.0 µM 1.23E-11 translation 29 5.42E-12
4 SGTC_1627 st003713 5.99 25.6 µM 2.02E-9 calcium & mitochondrial duress 30 1.04E-9
5 SGTC_832 4083-0019 5.91 164.0 µM 3.28E-9 translation 20 1.71E-9
6 SGTC_2741 miltefosine 5.74 3.0 µM 8.62E-9 21 4.63E-9
7 SGTC_2630 antimycin a 5.67 100.0 µM 1.30E-8 mitochondrial processes 15 7.08E-9
8 SGTC_1870 st057514 5.32 26.5 µM 8.83E-8 RSC & ERG11 8 5.09E-8
9 SGTC_309 k408-0051 4.94 1.5 µM 6.50E-7 30 3.99E-7
10 SGTC_1636 st007707 4.85 68.9 µM 1.01E-6 31 6.26E-7
11 SGTC_1025 k007-0731 4.83 70.0 µM 1.07E-6 5 6.68E-7
12 SGTC_791 1348-1292 4.71 225.0 µM 1.94E-6 20 1.23E-6
13 SGTC_701 4424-0219 4.65 185.0 µM 2.64E-6 calcium & mitochondrial duress 12 1.69E-6
14 SGTC_3281 9140613 4.51 49.5 µM 4.90E-6 RPP1 & pyrimidine depletion 11 3.20E-6
15 SGTC_2087 5276107 4.44 200.0 µM 6.71E-6 12 4.43E-6
16 SGTC_2092 5303694 4.34 80.7 µM 1.06E-5 TSC3-RPN4 30 7.11E-6
17 SGTC_801 0866-0028 4.24 10.1 µM 1.61E-5 20 1.09E-5
18 SGTC_2266 7929956 4.17 200.0 µM 2.23E-5 26 1.53E-5
19 SGTC_91 0417-1751 4.07 58.4 µM 3.36E-5 Golgi 50 2.33E-5
20 SGTC_240 5hr heat shock (37°C) + Streptovitacin 4.05 300.0 µM 3.74E-5 heat shock/prefoldin 38 2.60E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.231 5.14E-42 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.181 5.19E-26 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.153 4.07E-19 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.122 1.51E-12 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.122 1.53E-12 YHR059W FYV4 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.111 1.01E-10 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.105 1.10E-9 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.104 1.69E-9 YJR055W HIT1 hom Protein of unknown function, required for growth at high temperature
0.103 2.13E-9 YKL181W PRS1 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
0.100 6.77E-9 YBL007C SLA1 hom Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
0.100 7.10E-9 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.100 7.79E-9 YOL051W GAL11 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
0.099 9.34E-9 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.098 1.35E-8 YEL031W SPF1 hom P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
0.098 1.44E-8 YML031W NDC1 het Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1