YGR113W / DAM1

Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR113W / DAM1

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.82|P-value:7.85E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.54|P-value:3.92E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:3.61|P-value:2.88E-5 ID:SGTC_153|Compound:4077-0274|FD-Score:2.96|P-value:4.69E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-3.56|P-value:7.15E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:-3.13|P-value:4.24E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.04|P-value:3.39E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:2.87|P-value:6.47E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.41|P-value:7.12E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.10|P-value:2.67E-4 ID:SGTC_462|Compound:ag-879|FD-Score:3.08|P-value:2.84E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.41|P-value:7.03E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.84|P-value:2.01E-5 ID:SGTC_601|Compound:0986-0029|FD-Score:3.15|P-value:2.13E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.29|P-value:1.22E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.92|P-value:1.39E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:2.94|P-value:5.01E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.93|P-value:2.44E-8 ID:SGTC_695|Compound:3505-6186|FD-Score:4.93|P-value:2.41E-8 ID:SGTC_705|Compound:1124-0212|FD-Score:-3.03|P-value:6.35E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.23|P-value:1.56E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:5.34|P-value:1.73E-9 ID:SGTC_855|Compound:0428-0020|FD-Score:-3.29|P-value:2.30E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.86|P-value:8.98E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:-3.95|P-value:1.20E-5 ID:SGTC_895|Compound:0964-0006|FD-Score:3.28|P-value:1.26E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:5.60|P-value:3.00E-10 ID:SGTC_996|Compound:3970-0799|FD-Score:4.88|P-value:3.22E-8 ID:SGTC_1114|Compound:scopoletin|FD-Score:2.96|P-value:4.64E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.62|P-value:2.82E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.85|P-value:3.96E-8 ID:SGTC_1427|Compound:4048-4900|FD-Score:2.79|P-value:8.76E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:4.72|P-value:8.32E-8 ID:SGTC_1568|Compound:digitonin|FD-Score:3.68|P-value:2.11E-5 ID:SGTC_1716|Compound:st033144|FD-Score:2.79|P-value:8.93E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.08|P-value:5.32E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.11|P-value:4.60E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.01|P-value:3.76E-4 ID:SGTC_1822|Compound:st052468|FD-Score:-3.87|P-value:1.73E-5 ID:SGTC_1864|Compound:st057645|FD-Score:-3.09|P-value:5.03E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:2.95|P-value:4.79E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.49|P-value:3.26E-7 ID:SGTC_2164|Compound:5685496|FD-Score:2.90|P-value:5.79E-4 ID:SGTC_2226|Compound:7298446|FD-Score:2.77|P-value:9.47E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.24|P-value:1.23E-6 ID:SGTC_2292|Compound:7671323|FD-Score:5.90|P-value:3.52E-11 ID:SGTC_2302|Compound:7771753|FD-Score:-2.93|P-value:9.36E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-3.26|P-value:2.52E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.34|P-value:1.86E-4 ID:SGTC_2458|Compound:5808907|FD-Score:2.87|P-value:6.46E-4 ID:SGTC_2472|Compound:5377652|FD-Score:3.96|P-value:5.28E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.09|P-value:5.08E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.97|P-value:5.12E-6 ID:SGTC_2830|Compound:7992007|FD-Score:4.49|P-value:3.23E-7 ID:SGTC_2844|Compound:9010103|FD-Score:-4.41|P-value:1.13E-6 ID:SGTC_2845|Compound:9003024|FD-Score:-2.92|P-value:9.45E-4 ID:SGTC_2894|Compound:9048625|FD-Score:-3.71|P-value:3.71E-5 ID:SGTC_2918|Compound:7977171|FD-Score:2.88|P-value:6.44E-4 ID:SGTC_2958|Compound:9077254|FD-Score:3.17|P-value:1.99E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-2.95|P-value:8.71E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-2.96|P-value:8.26E-4 ID:SGTC_2976|Compound:9009005|FD-Score:5.12|P-value:7.33E-9 ID:SGTC_2981|Compound:9013952|FD-Score:-9.51|P-value:4.05E-25 ID:SGTC_2982|Compound:9019404|FD-Score:3.26|P-value:1.35E-4 ID:SGTC_3124|Compound:9127567|FD-Score:-3.44|P-value:1.20E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.28|P-value:2.41E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-3.26|P-value:2.57E-4 ID:SGTC_3193|Compound:9111931|FD-Score:5.25|P-value:3.07E-9 ID:SGTC_3247|Compound:9135149|FD-Score:3.27|P-value:1.29E-4 ID:SGTC_3255|Compound:9139072|FD-Score:-4.49|P-value:7.38E-7 ID:SGTC_3304|Compound:9125678|FD-Score:-3.70|P-value:3.82E-5 ID:SGTC_3310|Compound:9130229|FD-Score:3.47|P-value:5.43E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.82|P-value:7.85E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.54|P-value:3.92E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:3.61|P-value:2.88E-5 ID:SGTC_153|Compound:4077-0274|FD-Score:2.96|P-value:4.69E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-3.56|P-value:7.15E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:-3.13|P-value:4.24E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:3.04|P-value:3.39E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:2.87|P-value:6.47E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.41|P-value:7.12E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.10|P-value:2.67E-4 ID:SGTC_462|Compound:ag-879|FD-Score:3.08|P-value:2.84E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.41|P-value:7.03E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.84|P-value:2.01E-5 ID:SGTC_601|Compound:0986-0029|FD-Score:3.15|P-value:2.13E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.29|P-value:1.22E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.92|P-value:1.39E-5 ID:SGTC_632|Compound:0781-2353|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:2.94|P-value:5.01E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.93|P-value:2.44E-8 ID:SGTC_695|Compound:3505-6186|FD-Score:4.93|P-value:2.41E-8 ID:SGTC_705|Compound:1124-0212|FD-Score:-3.03|P-value:6.35E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.23|P-value:1.56E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:5.34|P-value:1.73E-9 ID:SGTC_855|Compound:0428-0020|FD-Score:-3.29|P-value:2.30E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.86|P-value:8.98E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:-3.95|P-value:1.20E-5 ID:SGTC_895|Compound:0964-0006|FD-Score:3.28|P-value:1.26E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:5.60|P-value:3.00E-10 ID:SGTC_996|Compound:3970-0799|FD-Score:4.88|P-value:3.22E-8 ID:SGTC_1114|Compound:scopoletin|FD-Score:2.96|P-value:4.64E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.62|P-value:2.82E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.85|P-value:3.96E-8 ID:SGTC_1427|Compound:4048-4900|FD-Score:2.79|P-value:8.76E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:4.72|P-value:8.32E-8 ID:SGTC_1568|Compound:digitonin|FD-Score:3.68|P-value:2.11E-5 ID:SGTC_1716|Compound:st033144|FD-Score:2.79|P-value:8.93E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.08|P-value:5.32E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.11|P-value:4.60E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.01|P-value:3.76E-4 ID:SGTC_1822|Compound:st052468|FD-Score:-3.87|P-value:1.73E-5 ID:SGTC_1864|Compound:st057645|FD-Score:-3.09|P-value:5.03E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:2.95|P-value:4.79E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.49|P-value:3.26E-7 ID:SGTC_2164|Compound:5685496|FD-Score:2.90|P-value:5.79E-4 ID:SGTC_2226|Compound:7298446|FD-Score:2.77|P-value:9.47E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.24|P-value:1.23E-6 ID:SGTC_2292|Compound:7671323|FD-Score:5.90|P-value:3.52E-11 ID:SGTC_2302|Compound:7771753|FD-Score:-2.93|P-value:9.36E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-3.26|P-value:2.52E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.34|P-value:1.86E-4 ID:SGTC_2458|Compound:5808907|FD-Score:2.87|P-value:6.46E-4 ID:SGTC_2472|Compound:5377652|FD-Score:3.96|P-value:5.28E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.09|P-value:5.08E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.97|P-value:5.12E-6 ID:SGTC_2830|Compound:7992007|FD-Score:4.49|P-value:3.23E-7 ID:SGTC_2844|Compound:9010103|FD-Score:-4.41|P-value:1.13E-6 ID:SGTC_2845|Compound:9003024|FD-Score:-2.92|P-value:9.45E-4 ID:SGTC_2894|Compound:9048625|FD-Score:-3.71|P-value:3.71E-5 ID:SGTC_2918|Compound:7977171|FD-Score:2.88|P-value:6.44E-4 ID:SGTC_2958|Compound:9077254|FD-Score:3.17|P-value:1.99E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-2.95|P-value:8.71E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-2.96|P-value:8.26E-4 ID:SGTC_2976|Compound:9009005|FD-Score:5.12|P-value:7.33E-9 ID:SGTC_2981|Compound:9013952|FD-Score:-9.51|P-value:4.05E-25 ID:SGTC_2982|Compound:9019404|FD-Score:3.26|P-value:1.35E-4 ID:SGTC_3124|Compound:9127567|FD-Score:-3.44|P-value:1.20E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.28|P-value:2.41E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-3.26|P-value:2.57E-4 ID:SGTC_3193|Compound:9111931|FD-Score:5.25|P-value:3.07E-9 ID:SGTC_3247|Compound:9135149|FD-Score:3.27|P-value:1.29E-4 ID:SGTC_3255|Compound:9139072|FD-Score:-4.49|P-value:7.38E-7 ID:SGTC_3304|Compound:9125678|FD-Score:-3.70|P-value:3.82E-5 ID:SGTC_3310|Compound:9130229|FD-Score:3.47|P-value:5.43E-5

Top fitness defect scores for YGR113W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2292 7671323 5.90 168.1 µM 3.52E-11 RNA processing & uracil transport 2 1.80E-9 0.12
2 SGTC_896 0964-0007 5.60 44.5 µM 3.00E-10 1 1.08E-8 0.91
3 SGTC_829 1635-0120 5.34 117.0 µM 1.73E-9 DNA damage response 31 4.64E-8 0.07
4 SGTC_3193 9111931 5.25 49.5 µM 3.07E-9 2 7.48E-8 0.31
5 SGTC_2976 9009005 5.12 19.5 µM 7.33E-9 1 1.54E-7 0.65
6 SGTC_695 3505-6186 4.93 243.0 µM 2.41E-8 3 4.16E-7 0.05
7 SGTC_682 0kpi-0099 4.93 17.0 µM 2.44E-8 19 4.20E-7 0.12
8 SGTC_996 3970-0799 4.88 1.8 µM 3.22E-8 16 5.30E-7 0.25
9 SGTC_1373 2887-0003 4.85 58.5 µM 3.96E-8 Golgi 7 6.29E-7 0.65
10 SGTC_1458 k015-0034 4.72 6.3 µM 8.32E-8 3 1.17E-6 1.31
11 SGTC_2830 7992007 4.49 71.4 µM 3.23E-7 9 3.61E-6 0.23
12 SGTC_1979 st071809 4.49 45.3 µM 3.26E-7 RPP1 & pyrimidine depletion 4 3.64E-6 0.01
13 SGTC_2233 sulbentine 4.24 40.4 µM 1.23E-6 34 1.10E-5 0.14
14 SGTC_2829 7992743 3.97 58.4 µM 5.12E-6 7 3.61E-5 0.19
15 SGTC_2472 5377652 3.96 49.4 µM 5.28E-6 TRP & mitochondrial translation 10 3.70E-5 0.22
16 SGTC_871 0574-0004 3.86 149.0 µM 8.98E-6 27 5.76E-5 0.02
17 SGTC_1568 digitonin 3.68 820.0 nM 2.11E-5 sphingolipid biosynthesis & PDR1 19 1.17E-4 0.02
18 SGTC_1162 k048-0130 3.62 20.1 µM 2.82E-5 Golgi 11 1.49E-4 0.14
19 SGTC_147 4466-0538 3.61 29.2 µM 2.88E-5 ergosterol biosynthesis 14 1.52E-4 0.09
20 SGTC_143 4032-1194 3.54 15.7 µM 3.92E-5 26 1.97E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.16E-18 YLL038C ENT4 hom Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
0.145 3.09E-17 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.131 2.37E-14 YBR135W CKS1 het Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
0.130 3.53E-14 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.128 8.44E-14 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.115 2.05E-11 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.113 4.57E-11 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.104 1.66E-9 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.103 2.38E-9 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.102 3.75E-9 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.099 9.33E-9 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.096 2.36E-8 YGL038C OCH1 hom Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
0.096 2.93E-8 YDR016C DAD1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.095 4.19E-8 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.092 8.38E-8 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance