YGR116W / SPT6

Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR116W / SPT6

Click on Significant Values for Screen Details ID:SGTC_115|Compound:0527-0192|FD-Score:4.32|P-value:7.12E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.62|P-value:2.36E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:3.77|P-value:4.66E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.73|P-value:5.31E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.54|P-value:9.49E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:3.65|P-value:6.88E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.01|P-value:4.96E-6 ID:SGTC_398|Compound:bronopol|FD-Score:-3.52|P-value:6.94E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.61|P-value:7.69E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-5.61|P-value:2.06E-7 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-9.55|P-value:4.80E-18 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-5.03|P-value:2.81E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:4.15|P-value:1.33E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:4.43|P-value:4.90E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:3.94|P-value:2.71E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:4.81|P-value:1.11E-5 ID:SGTC_696|Compound:1560-0013|FD-Score:4.05|P-value:1.86E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:5.32|P-value:1.31E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:5.89|P-value:9.73E-8 ID:SGTC_809|Compound:3998-0021|FD-Score:-4.72|P-value:1.01E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.70|P-value:5.78E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-4.36|P-value:3.96E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.54|P-value:6.50E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-5.26|P-value:1.03E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:-3.61|P-value:5.27E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:4.14|P-value:1.34E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:4.06|P-value:1.80E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.59|P-value:8.12E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.85|P-value:2.39E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.86|P-value:2.32E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.52|P-value:9.98E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.47|P-value:4.21E-5 ID:SGTC_1590|Compound:st077108|FD-Score:5.04|P-value:4.40E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-4.55|P-value:1.92E-5 ID:SGTC_1633|Compound:st005664|FD-Score:4.87|P-value:8.95E-6 ID:SGTC_1635|Compound:st007035|FD-Score:-3.64|P-value:4.75E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.68|P-value:6.22E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-4.00|P-value:1.46E-4 ID:SGTC_1748|Compound:st037455|FD-Score:4.22|P-value:1.03E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-4.15|P-value:8.55E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-3.70|P-value:4.00E-4 ID:SGTC_1758|Compound:st042942|FD-Score:4.12|P-value:1.46E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.69|P-value:1.43E-7 ID:SGTC_1871|Compound:st057448|FD-Score:3.75|P-value:4.93E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-4.77|P-value:8.03E-6 ID:SGTC_2007|Compound:4011539|FD-Score:4.79|P-value:1.21E-5 ID:SGTC_2009|Compound:4012297|FD-Score:5.44|P-value:7.71E-7 ID:SGTC_2015|Compound:5119600|FD-Score:3.71|P-value:5.66E-4 ID:SGTC_2085|Compound:5307070|FD-Score:4.07|P-value:1.72E-4 ID:SGTC_2267|Compound:7931562|FD-Score:-3.90|P-value:2.05E-4 ID:SGTC_2279|Compound:7948423|FD-Score:4.55|P-value:3.05E-5 ID:SGTC_2310|Compound:7668677|FD-Score:4.07|P-value:1.75E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.45|P-value:4.50E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-4.19|P-value:7.30E-5 ID:SGTC_2549|Compound:cedrol|FD-Score:4.28|P-value:8.18E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.77|P-value:4.60E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:3.76|P-value:4.84E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.22|P-value:1.03E-4 ID:SGTC_2964|Compound:9083345|FD-Score:4.39|P-value:5.55E-5 ID:SGTC_2980|Compound:9014342|FD-Score:5.37|P-value:1.07E-6 ID:SGTC_2989|Compound:9073649|FD-Score:3.74|P-value:5.10E-4 ID:SGTC_3009|Compound:9080819|FD-Score:4.15|P-value:1.32E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.15|P-value:1.33E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:4.32|P-value:7.12E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.62|P-value:2.36E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:3.77|P-value:4.66E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.73|P-value:5.31E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:3.54|P-value:9.49E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:3.65|P-value:6.88E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.01|P-value:4.96E-6 ID:SGTC_398|Compound:bronopol|FD-Score:-3.52|P-value:6.94E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.61|P-value:7.69E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:-5.61|P-value:2.06E-7 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-9.55|P-value:4.80E-18 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-5.03|P-value:2.81E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:4.15|P-value:1.33E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:4.43|P-value:4.90E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:3.94|P-value:2.71E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:4.81|P-value:1.11E-5 ID:SGTC_696|Compound:1560-0013|FD-Score:4.05|P-value:1.86E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:5.32|P-value:1.31E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:5.89|P-value:9.73E-8 ID:SGTC_809|Compound:3998-0021|FD-Score:-4.72|P-value:1.01E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.70|P-value:5.78E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-4.36|P-value:3.96E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.54|P-value:6.50E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-5.26|P-value:1.03E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:-3.61|P-value:5.27E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:4.14|P-value:1.34E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:4.06|P-value:1.80E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.59|P-value:8.12E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.85|P-value:2.39E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.86|P-value:2.32E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.52|P-value:9.98E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.47|P-value:4.21E-5 ID:SGTC_1590|Compound:st077108|FD-Score:5.04|P-value:4.40E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-4.55|P-value:1.92E-5 ID:SGTC_1633|Compound:st005664|FD-Score:4.87|P-value:8.95E-6 ID:SGTC_1635|Compound:st007035|FD-Score:-3.64|P-value:4.75E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.68|P-value:6.22E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-4.00|P-value:1.46E-4 ID:SGTC_1748|Compound:st037455|FD-Score:4.22|P-value:1.03E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-4.15|P-value:8.55E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-3.70|P-value:4.00E-4 ID:SGTC_1758|Compound:st042942|FD-Score:4.12|P-value:1.46E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.69|P-value:1.43E-7 ID:SGTC_1871|Compound:st057448|FD-Score:3.75|P-value:4.93E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-4.77|P-value:8.03E-6 ID:SGTC_2007|Compound:4011539|FD-Score:4.79|P-value:1.21E-5 ID:SGTC_2009|Compound:4012297|FD-Score:5.44|P-value:7.71E-7 ID:SGTC_2015|Compound:5119600|FD-Score:3.71|P-value:5.66E-4 ID:SGTC_2085|Compound:5307070|FD-Score:4.07|P-value:1.72E-4 ID:SGTC_2267|Compound:7931562|FD-Score:-3.90|P-value:2.05E-4 ID:SGTC_2279|Compound:7948423|FD-Score:4.55|P-value:3.05E-5 ID:SGTC_2310|Compound:7668677|FD-Score:4.07|P-value:1.75E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.45|P-value:4.50E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-4.19|P-value:7.30E-5 ID:SGTC_2549|Compound:cedrol|FD-Score:4.28|P-value:8.18E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.77|P-value:4.60E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:3.76|P-value:4.84E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.22|P-value:1.03E-4 ID:SGTC_2964|Compound:9083345|FD-Score:4.39|P-value:5.55E-5 ID:SGTC_2980|Compound:9014342|FD-Score:5.37|P-value:1.07E-6 ID:SGTC_2989|Compound:9073649|FD-Score:3.74|P-value:5.10E-4 ID:SGTC_3009|Compound:9080819|FD-Score:4.15|P-value:1.32E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.15|P-value:1.33E-4

Top fitness defect scores for YGR116W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_800 3448-7362 5.89 155.0 µM 9.73E-8 translation 23 1.91E-9 0.05
2 SGTC_2009 4012297 5.44 12.1 µM 7.71E-7 cell wall signaling 4 2.60E-8 0.23
3 SGTC_2980 9014342 5.37 25.3 µM 1.07E-6 9 3.93E-8 0.28
4 SGTC_795 0685-0265 5.32 78.0 µM 1.31E-6 excess fatty acid 5 5.08E-8 0.03
5 SGTC_1590 st077108 5.04 57.8 µM 4.40E-6 3 2.33E-7 0.86
6 SGTC_173 1273-0061 5.01 2.7 µM 4.96E-6 mitochondrial stress 12 2.70E-7 0.06
7 SGTC_1633 st005664 4.87 67.9 µM 8.95E-6 2 5.68E-7 0.55
8 SGTC_692 k241-0027 4.81 77.8 µM 1.11E-5 2 7.44E-7 0.28
9 SGTC_2007 4011539 4.79 71.8 µM 1.21E-5 cell wall signaling 4 8.33E-7 0.02
10 SGTC_120 rhodamine 6G 4.62 52.3 µM 2.36E-5 mitochondrial processes 11 1.92E-6 0.06
11 SGTC_2279 7948423 4.55 32.9 µM 3.05E-5 4 2.66E-6 0.23
12 SGTC_1371 2421-0008 4.47 32.7 µM 4.21E-5 mitochondrial processes 20 3.99E-6 0.24
13 SGTC_2399 5884133 4.45 200.0 µM 4.50E-5 cell wall signaling 14 4.34E-6 0.13
14 SGTC_626 0214-0009 4.43 33.0 µM 4.90E-5 copper-dependent oxidative stress 1 4.82E-6 0.42
15 SGTC_2964 9083345 4.39 53.4 µM 5.55E-5 9 5.65E-6 0.09
16 SGTC_115 0527-0192 4.32 112.0 µM 7.12E-5 19 7.72E-6 0.07
17 SGTC_2549 cedrol 4.28 20.9 µM 8.18E-5 7 9.19E-6 0.14
18 SGTC_1748 st037455 4.22 53.6 µM 1.03E-4 7 1.23E-5 0.24
19 SGTC_2833 9001315 4.22 45.5 µM 1.03E-4 amide catabolism 6 1.23E-5 0.13
20 SGTC_3009 9080819 4.15 71.4 µM 1.32E-4 9 1.68E-5 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.259 2.16E-52 YGR114C_d YGR114C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
0.190 1.30E-28 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.167 2.16E-22 YPR133C SPN1 het Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
0.140 3.89E-16 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.132 1.59E-14 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.126 2.34E-13 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.123 8.11E-13 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.121 1.93E-12 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.118 6.78E-12 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.116 1.51E-11 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.110 1.98E-10 YNL114C_d YNL114C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
0.108 4.12E-10 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.105 9.92E-10 YGL025C PGD1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.101 3.87E-9 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
0.101 4.85E-9 YGL064C MRH4 hom Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function