YGR124W / ASN2

Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR124W / ASN2

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:-3.26|P-value:5.97E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.35|P-value:1.15E-10 ID:SGTC_60|Compound:0986-0278|FD-Score:4.23|P-value:1.06E-5 ID:SGTC_94|Compound:0334-0113|FD-Score:-3.27|P-value:5.72E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.42|P-value:2.90E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.70|P-value:9.66E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.78|P-value:6.99E-5 ID:SGTC_302|Compound:1094-0063|FD-Score:-4.07|P-value:2.52E-5 ID:SGTC_448|Compound:monastrol|FD-Score:-3.25|P-value:5.99E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.90|P-value:4.28E-5 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.34|P-value:4.50E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-4.80|P-value:8.63E-7 ID:SGTC_788|Compound:1182-0090|FD-Score:3.83|P-value:5.84E-5 ID:SGTC_826|Compound:1683-5028|FD-Score:3.79|P-value:6.91E-5 ID:SGTC_918|Compound:glabranin|FD-Score:4.90|P-value:4.12E-7 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.58|P-value:1.57E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.50|P-value:3.63E-6 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:4.23|P-value:1.02E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.11|P-value:8.52E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-4.72|P-value:1.27E-6 ID:SGTC_1728|Compound:st035614|FD-Score:-3.81|P-value:7.33E-5 ID:SGTC_1766|Compound:st045414|FD-Score:3.51|P-value:2.01E-4 ID:SGTC_1783|Compound:st039459|FD-Score:4.05|P-value:2.28E-5 ID:SGTC_1937|Compound:st074714|FD-Score:3.52|P-value:1.98E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.13|P-value:1.62E-5 ID:SGTC_2106|Compound:5357045|FD-Score:-4.12|P-value:1.98E-5 ID:SGTC_2109|Compound:5483026|FD-Score:5.11|P-value:1.37E-7 ID:SGTC_2112|Compound:5488546|FD-Score:-3.62|P-value:1.53E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.11|P-value:8.48E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.18|P-value:6.84E-4 ID:SGTC_2387|Compound:9077560|FD-Score:3.26|P-value:5.04E-4 ID:SGTC_2526|Compound:patulin|FD-Score:-3.11|P-value:9.85E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.13|P-value:8.12E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-4.13|P-value:1.97E-5 ID:SGTC_2702|Compound:st077768|FD-Score:3.15|P-value:7.56E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:5.58|P-value:1.05E-8 ID:SGTC_2812|Compound:7970281|FD-Score:-3.99|P-value:3.55E-5 ID:SGTC_2815|Compound:7968870|FD-Score:3.51|P-value:2.02E-4 ID:SGTC_3011|Compound:9081274|FD-Score:3.48|P-value:2.27E-4 ID:SGTC_3027|Compound:9089541|FD-Score:4.70|P-value:1.12E-6 ID:SGTC_3041|Compound:9091929|FD-Score:4.72|P-value:1.01E-6 ID:SGTC_3050|Compound:9093513|FD-Score:3.47|P-value:2.41E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.22|P-value:5.97E-4 ID:SGTC_3218|Compound:9131285|FD-Score:3.33|P-value:3.94E-4 ID:SGTC_3232|Compound:9132874|FD-Score:4.42|P-value:4.34E-6 ID:SGTC_55|Compound:0767-0285|FD-Score:-3.26|P-value:5.97E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.35|P-value:1.15E-10 ID:SGTC_60|Compound:0986-0278|FD-Score:4.23|P-value:1.06E-5 ID:SGTC_94|Compound:0334-0113|FD-Score:-3.27|P-value:5.72E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.42|P-value:2.90E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.70|P-value:9.66E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.78|P-value:6.99E-5 ID:SGTC_302|Compound:1094-0063|FD-Score:-4.07|P-value:2.52E-5 ID:SGTC_448|Compound:monastrol|FD-Score:-3.25|P-value:5.99E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:3.90|P-value:4.28E-5 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.34|P-value:4.50E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-4.80|P-value:8.63E-7 ID:SGTC_788|Compound:1182-0090|FD-Score:3.83|P-value:5.84E-5 ID:SGTC_826|Compound:1683-5028|FD-Score:3.79|P-value:6.91E-5 ID:SGTC_918|Compound:glabranin|FD-Score:4.90|P-value:4.12E-7 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.58|P-value:1.57E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.50|P-value:3.63E-6 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:4.23|P-value:1.02E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.11|P-value:8.52E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-4.72|P-value:1.27E-6 ID:SGTC_1728|Compound:st035614|FD-Score:-3.81|P-value:7.33E-5 ID:SGTC_1766|Compound:st045414|FD-Score:3.51|P-value:2.01E-4 ID:SGTC_1783|Compound:st039459|FD-Score:4.05|P-value:2.28E-5 ID:SGTC_1937|Compound:st074714|FD-Score:3.52|P-value:1.98E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.13|P-value:1.62E-5 ID:SGTC_2106|Compound:5357045|FD-Score:-4.12|P-value:1.98E-5 ID:SGTC_2109|Compound:5483026|FD-Score:5.11|P-value:1.37E-7 ID:SGTC_2112|Compound:5488546|FD-Score:-3.62|P-value:1.53E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.11|P-value:8.48E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.18|P-value:6.84E-4 ID:SGTC_2387|Compound:9077560|FD-Score:3.26|P-value:5.04E-4 ID:SGTC_2526|Compound:patulin|FD-Score:-3.11|P-value:9.85E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.13|P-value:8.12E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-4.13|P-value:1.97E-5 ID:SGTC_2702|Compound:st077768|FD-Score:3.15|P-value:7.56E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:5.58|P-value:1.05E-8 ID:SGTC_2812|Compound:7970281|FD-Score:-3.99|P-value:3.55E-5 ID:SGTC_2815|Compound:7968870|FD-Score:3.51|P-value:2.02E-4 ID:SGTC_3011|Compound:9081274|FD-Score:3.48|P-value:2.27E-4 ID:SGTC_3027|Compound:9089541|FD-Score:4.70|P-value:1.12E-6 ID:SGTC_3041|Compound:9091929|FD-Score:4.72|P-value:1.01E-6 ID:SGTC_3050|Compound:9093513|FD-Score:3.47|P-value:2.41E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.22|P-value:5.97E-4 ID:SGTC_3218|Compound:9131285|FD-Score:3.33|P-value:3.94E-4 ID:SGTC_3232|Compound:9132874|FD-Score:4.42|P-value:4.34E-6

Top fitness defect scores for YGR124W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2750 bromebric acid 5.58 70.2 µM 1.05E-8 7 1.23E-8
2 SGTC_2109 5483026 5.11 200.0 µM 1.37E-7 RSC complex & mRNA processing 10 1.59E-7
3 SGTC_918 glabranin 4.90 770.7 nM 4.12E-7 11 4.72E-7
4 SGTC_3041 9091929 4.72 49.5 µM 1.01E-6 redox potentiating 11 1.16E-6
5 SGTC_3027 9089541 4.70 49.5 µM 1.12E-6 fatty acid desaturase (OLE1) 10 1.27E-6
6 SGTC_3232 9132874 4.42 49.5 µM 4.34E-6 RPP1 & pyrimidine depletion 9 4.90E-6
7 SGTC_1252 0493-0875 4.23 34.8 µM 1.02E-5 22 1.15E-5
8 SGTC_60 0986-0278 4.23 202.0 µM 1.06E-5 9 1.19E-5
9 SGTC_1994 st072947 4.13 40.9 µM 1.62E-5 11 1.81E-5
10 SGTC_1783 st039459 4.05 87.5 µM 2.28E-5 Golgi 21 2.54E-5
11 SGTC_589 1227-0070 3.90 34.1 µM 4.28E-5 30 4.76E-5
12 SGTC_788 1182-0090 3.83 188.0 µM 5.84E-5 endomembrane recycling 81 6.48E-5
13 SGTC_826 1683-5028 3.79 118.0 µM 6.91E-5 20 7.65E-5
14 SGTC_234 nsc-17383 3.78 10.7 µM 6.99E-5 ERG2 27 7.74E-5
15 SGTC_159 3970-0959 3.70 50.0 µM 9.66E-5 90 1.07E-4
16 SGTC_1053 3366-3204 3.58 134.0 µM 1.57E-4 cell wall 48 1.72E-4
17 SGTC_1230 0170-0365 3.53 3.8 µM 1.91E-4 endomembrane recycling 89 2.10E-4
18 SGTC_1937 st074714 3.52 47.2 µM 1.98E-4 54 2.17E-4
19 SGTC_1766 st045414 3.51 44.8 µM 2.01E-4 RPP1 & pyrimidine depletion 45 2.20E-4
20 SGTC_2815 7968870 3.51 71.4 µM 2.02E-4 41 2.22E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.39E-12 YKL074C MUD2 hom Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
0.120 3.17E-12 YOL079W_d YOL079W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.107 5.33E-10 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.106 6.83E-10 YDR156W RPA14 hom RNA polymerase I subunit A14
0.106 7.47E-10 YOL158C ENB1 hom Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
0.106 8.58E-10 YDR147W EKI1 hom Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
0.105 1.07E-9 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.105 1.24E-9 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.103 2.03E-9 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.094 5.06E-8 YLL037W_d YLL037W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
0.091 1.45E-7 YPL079W RPL21B hom Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
0.086 6.07E-7 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.084 1.20E-6 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.084 1.28E-6 YNL097C PHO23 hom Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3
0.083 1.29E-6 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation