YGR133W / PEX4

Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR133W / PEX4

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-2.91|P-value:6.68E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.11|P-value:3.16E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:-3.07|P-value:3.59E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.05|P-value:3.82E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:6.36|P-value:1.46E-12 ID:SGTC_393|Compound:acivicin|FD-Score:-3.73|P-value:1.98E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-2.90|P-value:7.07E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-2.98|P-value:5.09E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:-2.81|P-value:9.83E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-2.88|P-value:7.41E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.08|P-value:3.69E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.52|P-value:5.53E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:4.37|P-value:7.97E-7 ID:SGTC_649|Compound:1123-0311|FD-Score:4.43|P-value:5.60E-7 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.36|P-value:1.06E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:2.94|P-value:6.22E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.50|P-value:5.75E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:2.92|P-value:6.71E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.69|P-value:2.54E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:4.83|P-value:5.58E-8 ID:SGTC_828|Compound:2074-0507|FD-Score:3.61|P-value:3.65E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.06|P-value:3.89E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:4.64|P-value:1.76E-7 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.44|P-value:8.00E-5 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.25|P-value:1.81E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.51|P-value:5.88E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.53|P-value:5.34E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.40|P-value:9.63E-5 ID:SGTC_1422|Compound:4031-0024|FD-Score:3.55|P-value:4.99E-5 ID:SGTC_1526|Compound:st051518|FD-Score:-3.19|P-value:2.19E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.65|P-value:3.06E-5 ID:SGTC_1720|Compound:st037298|FD-Score:5.18|P-value:6.24E-9 ID:SGTC_1723|Compound:st034307|FD-Score:-6.44|P-value:6.71E-13 ID:SGTC_1729|Compound:st035615|FD-Score:3.21|P-value:2.15E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.55|P-value:4.84E-5 ID:SGTC_1887|Compound:st057648|FD-Score:-2.92|P-value:6.50E-4 ID:SGTC_1893|Compound:st059353|FD-Score:-3.01|P-value:4.49E-4 ID:SGTC_1939|Compound:st074455|FD-Score:2.95|P-value:6.12E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.03|P-value:4.22E-4 ID:SGTC_2201|Compound:6605497|FD-Score:2.85|P-value:8.76E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-2.82|P-value:9.60E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.35|P-value:1.17E-4 ID:SGTC_2333|Compound:9017922|FD-Score:3.06|P-value:4.00E-4 ID:SGTC_2350|Compound:9023895|FD-Score:2.92|P-value:6.84E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.28|P-value:1.61E-4 ID:SGTC_2384|Compound:9036245|FD-Score:2.98|P-value:5.34E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.86|P-value:8.64E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-2.92|P-value:6.57E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-4.25|P-value:1.47E-6 ID:SGTC_3304|Compound:9125678|FD-Score:-3.03|P-value:4.21E-4 ID:SGTC_3307|Compound:9126885|FD-Score:-3.30|P-value:1.38E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.60|P-value:3.97E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-2.91|P-value:6.68E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.11|P-value:3.16E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:-3.07|P-value:3.59E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.05|P-value:3.82E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:6.36|P-value:1.46E-12 ID:SGTC_393|Compound:acivicin|FD-Score:-3.73|P-value:1.98E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-2.90|P-value:7.07E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-2.98|P-value:5.09E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:-2.81|P-value:9.83E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-2.88|P-value:7.41E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.08|P-value:3.69E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.52|P-value:5.53E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:4.37|P-value:7.97E-7 ID:SGTC_649|Compound:1123-0311|FD-Score:4.43|P-value:5.60E-7 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.36|P-value:1.06E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:2.94|P-value:6.22E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.50|P-value:5.75E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:2.92|P-value:6.71E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.69|P-value:2.54E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:4.83|P-value:5.58E-8 ID:SGTC_828|Compound:2074-0507|FD-Score:3.61|P-value:3.65E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.06|P-value:3.89E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:4.64|P-value:1.76E-7 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.44|P-value:8.00E-5 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.25|P-value:1.81E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.51|P-value:5.88E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.53|P-value:5.34E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.40|P-value:9.63E-5 ID:SGTC_1422|Compound:4031-0024|FD-Score:3.55|P-value:4.99E-5 ID:SGTC_1526|Compound:st051518|FD-Score:-3.19|P-value:2.19E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.65|P-value:3.06E-5 ID:SGTC_1720|Compound:st037298|FD-Score:5.18|P-value:6.24E-9 ID:SGTC_1723|Compound:st034307|FD-Score:-6.44|P-value:6.71E-13 ID:SGTC_1729|Compound:st035615|FD-Score:3.21|P-value:2.15E-4 ID:SGTC_1734|Compound:st037168|FD-Score:3.55|P-value:4.84E-5 ID:SGTC_1887|Compound:st057648|FD-Score:-2.92|P-value:6.50E-4 ID:SGTC_1893|Compound:st059353|FD-Score:-3.01|P-value:4.49E-4 ID:SGTC_1939|Compound:st074455|FD-Score:2.95|P-value:6.12E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.03|P-value:4.22E-4 ID:SGTC_2201|Compound:6605497|FD-Score:2.85|P-value:8.76E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-2.82|P-value:9.60E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.35|P-value:1.17E-4 ID:SGTC_2333|Compound:9017922|FD-Score:3.06|P-value:4.00E-4 ID:SGTC_2350|Compound:9023895|FD-Score:2.92|P-value:6.84E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.28|P-value:1.61E-4 ID:SGTC_2384|Compound:9036245|FD-Score:2.98|P-value:5.34E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.86|P-value:8.64E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-2.92|P-value:6.57E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-4.25|P-value:1.47E-6 ID:SGTC_3304|Compound:9125678|FD-Score:-3.03|P-value:4.21E-4 ID:SGTC_3307|Compound:9126885|FD-Score:-3.30|P-value:1.38E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.60|P-value:3.97E-5

Top fitness defect scores for YGR133W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_383 2324-0395 6.36 7.4 µM 1.46E-12 azole & statin 15 1.04E-10
2 SGTC_1720 st037298 5.18 9.5 µM 6.24E-9 19 1.09E-7
3 SGTC_822 1487-0950 4.83 154.0 µM 5.58E-8 3 6.74E-7
4 SGTC_1039 k821-0043 4.64 94.0 µM 1.76E-7 Golgi 12 1.75E-6
5 SGTC_649 1123-0311 4.43 367.0 µM 5.60E-7 endomembrane recycling 52 4.60E-6
6 SGTC_648 0375-0473 4.37 33.3 µM 7.97E-7 11 6.18E-6
7 SGTC_813 k007-0158 3.69 173.0 µM 2.54E-5 fatty acid desaturase (OLE1) 54 1.11E-4
8 SGTC_1636 st007707 3.65 68.9 µM 3.06E-5 57 1.30E-4
9 SGTC_828 2074-0507 3.61 139.0 µM 3.65E-5 RPP1 & pyrimidine depletion 31 1.51E-4
10 SGTC_3315 9136395 3.60 68.5 µM 3.97E-5 15 1.62E-4
11 SGTC_1734 st037168 3.55 69.4 µM 4.84E-5 38 1.91E-4
12 SGTC_1422 4031-0024 3.55 15.5 µM 4.99E-5 iron homeostasis 26 1.96E-4
13 SGTC_1310 1188-0297 3.53 994.0 nM 5.34E-5 azole & statin 43 2.08E-4
14 SGTC_641 4092-0595 3.52 30.0 µM 5.53E-5 21 2.14E-4
15 SGTC_1308 1187-1617 3.51 41.4 µM 5.88E-5 fatty acid desaturase (OLE1) 62 2.25E-4
16 SGTC_1083 didemethylchlorpromazine sulfoxide 3.44 707.4 nM 8.00E-5 redox potentiating 110 2.91E-4
17 SGTC_1313 1220-0047 3.40 237.0 µM 9.63E-5 60 3.40E-4
18 SGTC_2331 7980721 3.35 200.0 µM 1.17E-4 48 4.00E-4
19 SGTC_2363 9053361 3.28 176.0 µM 1.61E-4 Golgi 58 5.24E-4
20 SGTC_1212 0520-0165 3.25 585.0 µM 1.81E-4 66 5.76E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.157 5.25E-20 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.151 1.28E-18 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.142 1.39E-16 YGR181W TIM13 hom Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
0.137 1.55E-15 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.130 4.24E-14 YHR045W_p YHR045W_p hom Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
0.123 8.48E-13 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.116 1.53E-11 YAL054C ACS1 hom Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
0.110 1.95E-10 YMR253C_p YMR253C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
0.099 1.02E-8 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.097 1.97E-8 YNL007C SIS1 het Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
0.095 3.08E-8 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.093 7.20E-8 YLL050C COF1 het Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
0.092 1.01E-7 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.090 1.90E-7 YMR162C DNF3 hom Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase
0.090 1.98E-7 YJL162C JJJ2 hom Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein