HIPHOP chemogenomics database

YGR135W / PRE9

Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform

Zygosity: Homozygous strain

fixedexpanded
Profile for YGR135W / PRE9

Click on Significant Values for Screen Details

Top fitness defect scores for YGR135W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2250	alverine citrate	11.60	130.0 µM	4.86E-31		4	3.10E-31
2	SGTC_1161	k007-0610	9.89	110.0 µM	3.19E-23	ERAD & cell cycle	16	2.26E-23
3	SGTC_103	0844-0104	9.49	51.8 µM	1.65E-21	ERAD & cell cycle	33	1.20E-21
4	SGTC_207	4185-0010	7.49	4.5 µM	4.29E-14	endomembrane recycling	31	3.46E-14
5	SGTC_2688	kanamycin b	6.52	96.7 µM	4.10E-11	calcium & mitochondrial duress	128	3.45E-11
6	SGTC_2563	tomatine	5.78	8.5 µM	4.26E-9		10	3.70E-9
7	SGTC_2795	7780010	5.28	54.5 µM	7.26E-8		6	6.42E-8
8	SGTC_187	1488-2104	5.10	32.7 µM	1.92E-7	calcium & mitochondrial duress	21	1.71E-7
9	SGTC_201	4262-0159	4.67	12.8 µM	1.68E-6	ergosterol depletion effects on membrane	32	1.52E-6
10	SGTC_292	k038-0015	4.63	474.0 µM	2.03E-6		2	1.83E-6
11	SGTC_2801	7907800	4.56	81.8 µM	2.79E-6		19	2.52E-6
12	SGTC_1923	st058458	4.47	19.1 µM	4.38E-6		21	3.98E-6
13	SGTC_2702	st077768	4.46	96.8 µM	4.43E-6		11	4.03E-6
14	SGTC_568	1193-0097	4.37	7.5 µM	6.72E-6	ERAD & cell cycle	121	6.12E-6
15	SGTC_689	0833-0039	4.34	98.0 µM	7.66E-6	ergosterol depletion effects on membrane	28	6.99E-6
16	SGTC_320	4092-0846	4.23	5.3 µM	1.30E-5	ergosterol depletion effects on membrane	42	1.19E-5
17	SGTC_8	0354-0001	4.19	70.0 µM	1.55E-5		71	1.42E-5
18	SGTC_2915	7971052	4.14	15.2 µM	1.92E-5		4	1.76E-5
19	SGTC_2086	5274770	4.08	176.6 µM	2.49E-5		22	2.29E-5
20	SGTC_2871	9038510	3.99	13.0 µM	3.63E-5		14	3.35E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.309	2.22E-75	YDL148C	NOP14	het	Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.258	4.39E-52	YOL065C	INP54	hom	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.252	8.50E-50	YLR021W	IRC25	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.241	1.06E-45	YDL001W	RMD1	hom	Cytoplasmic protein required for sporulation
0.224	2.68E-39	YBL010C_p	YBL010C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.215	2.71E-36	YHL019C	APM2	hom	Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.206	1.94E-33	YLR170C	APS1	hom	Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.206	2.08E-33	YOR363C	PIP2	hom	Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.206	2.46E-33	YFL034W_p	YFL034W_p	hom	Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.184	6.62E-27	YDR186C	YDR186C	hom	Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.176	1.06E-24	YNL239W	LAP3	hom	Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
0.175	1.66E-24	YJL001W	PRE3	het	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.172	1.28E-23	YMR029C	FAR8	hom	Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.169	8.39E-23	YER041W	YEN1	hom	Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.168	1.21E-22	YDL126C	CDC48	het	AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP