YGR135W / PRE9

Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR135W / PRE9

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.34|P-value:4.50E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:4.19|P-value:1.55E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:9.49|P-value:1.65E-21 ID:SGTC_162|Compound:k030-0015|FD-Score:-3.45|P-value:2.81E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-5.89|P-value:1.99E-9 ID:SGTC_166|Compound:k072-0058|FD-Score:-5.16|P-value:1.23E-7 ID:SGTC_187|Compound:1488-2104|FD-Score:5.10|P-value:1.92E-7 ID:SGTC_201|Compound:4262-0159|FD-Score:4.67|P-value:1.68E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:7.49|P-value:4.29E-14 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.26|P-value:5.92E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:4.63|P-value:2.03E-6 ID:SGTC_293|Compound:4049-0273|FD-Score:-4.11|P-value:2.04E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.28|P-value:5.11E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.12|P-value:9.62E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.74|P-value:9.86E-5 ID:SGTC_320|Compound:4092-0846|FD-Score:4.23|P-value:1.30E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.01|P-value:9.65E-10 ID:SGTC_532|Compound:1548-0774|FD-Score:3.35|P-value:4.31E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.37|P-value:6.72E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:3.37|P-value:4.04E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:-3.09|P-value:9.89E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:-3.58|P-value:1.72E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:4.34|P-value:7.66E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.16|P-value:1.62E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.21|P-value:6.63E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.11|P-value:9.96E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:9.89|P-value:3.19E-23 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.37|P-value:3.70E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.43|P-value:3.28E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.48|P-value:2.46E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.34|P-value:4.44E-4 ID:SGTC_1896|Compound:st058441|FD-Score:-3.13|P-value:8.64E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.47|P-value:4.38E-6 ID:SGTC_1930|Compound:st074634|FD-Score:-3.71|P-value:1.05E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-3.10|P-value:9.60E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.21|P-value:7.01E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.08|P-value:2.49E-5 ID:SGTC_2200|Compound:6600604|FD-Score:3.31|P-value:5.02E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:11.60|P-value:4.86E-31 ID:SGTC_2322|Compound:7413319|FD-Score:-7.39|P-value:7.89E-14 ID:SGTC_2358|Compound:9037157|FD-Score:3.80|P-value:7.93E-5 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.41|P-value:3.27E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.78|P-value:4.26E-9 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.92|P-value:4.51E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.32|P-value:4.48E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.52|P-value:4.10E-11 ID:SGTC_2702|Compound:st077768|FD-Score:4.46|P-value:4.43E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:3.61|P-value:1.64E-4 ID:SGTC_2795|Compound:7780010|FD-Score:5.28|P-value:7.26E-8 ID:SGTC_2801|Compound:7907800|FD-Score:4.56|P-value:2.79E-6 ID:SGTC_2871|Compound:9038510|FD-Score:3.99|P-value:3.63E-5 ID:SGTC_2915|Compound:7971052|FD-Score:4.14|P-value:1.92E-5 ID:SGTC_3182|Compound:9108849|FD-Score:-3.72|P-value:9.88E-5 ID:SGTC_3189|Compound:9110682|FD-Score:-3.27|P-value:5.30E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-4.44|P-value:4.51E-6 ID:SGTC_3|Compound:0833-0587|FD-Score:3.34|P-value:4.50E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:4.19|P-value:1.55E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:9.49|P-value:1.65E-21 ID:SGTC_162|Compound:k030-0015|FD-Score:-3.45|P-value:2.81E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-5.89|P-value:1.99E-9 ID:SGTC_166|Compound:k072-0058|FD-Score:-5.16|P-value:1.23E-7 ID:SGTC_187|Compound:1488-2104|FD-Score:5.10|P-value:1.92E-7 ID:SGTC_201|Compound:4262-0159|FD-Score:4.67|P-value:1.68E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:7.49|P-value:4.29E-14 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.26|P-value:5.92E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:4.63|P-value:2.03E-6 ID:SGTC_293|Compound:4049-0273|FD-Score:-4.11|P-value:2.04E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.28|P-value:5.11E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.12|P-value:9.62E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.74|P-value:9.86E-5 ID:SGTC_320|Compound:4092-0846|FD-Score:4.23|P-value:1.30E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.01|P-value:9.65E-10 ID:SGTC_532|Compound:1548-0774|FD-Score:3.35|P-value:4.31E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.37|P-value:6.72E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:3.37|P-value:4.04E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:-3.09|P-value:9.89E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:-3.58|P-value:1.72E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:4.34|P-value:7.66E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.16|P-value:1.62E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.21|P-value:6.63E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.11|P-value:9.96E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:9.89|P-value:3.19E-23 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.37|P-value:3.70E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.43|P-value:3.28E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.48|P-value:2.46E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.34|P-value:4.44E-4 ID:SGTC_1896|Compound:st058441|FD-Score:-3.13|P-value:8.64E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.47|P-value:4.38E-6 ID:SGTC_1930|Compound:st074634|FD-Score:-3.71|P-value:1.05E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-3.10|P-value:9.60E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.21|P-value:7.01E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.08|P-value:2.49E-5 ID:SGTC_2200|Compound:6600604|FD-Score:3.31|P-value:5.02E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:11.60|P-value:4.86E-31 ID:SGTC_2322|Compound:7413319|FD-Score:-7.39|P-value:7.89E-14 ID:SGTC_2358|Compound:9037157|FD-Score:3.80|P-value:7.93E-5 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.41|P-value:3.27E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.78|P-value:4.26E-9 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.92|P-value:4.51E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.32|P-value:4.48E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.52|P-value:4.10E-11 ID:SGTC_2702|Compound:st077768|FD-Score:4.46|P-value:4.43E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:3.61|P-value:1.64E-4 ID:SGTC_2795|Compound:7780010|FD-Score:5.28|P-value:7.26E-8 ID:SGTC_2801|Compound:7907800|FD-Score:4.56|P-value:2.79E-6 ID:SGTC_2871|Compound:9038510|FD-Score:3.99|P-value:3.63E-5 ID:SGTC_2915|Compound:7971052|FD-Score:4.14|P-value:1.92E-5 ID:SGTC_3182|Compound:9108849|FD-Score:-3.72|P-value:9.88E-5 ID:SGTC_3189|Compound:9110682|FD-Score:-3.27|P-value:5.30E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-4.44|P-value:4.51E-6

Top fitness defect scores for YGR135W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2250 alverine citrate 11.60 130.0 µM 4.86E-31 4 3.10E-31
2 SGTC_1161 k007-0610 9.89 110.0 µM 3.19E-23 ERAD & cell cycle 16 2.26E-23
3 SGTC_103 0844-0104 9.49 51.8 µM 1.65E-21 ERAD & cell cycle 33 1.20E-21
4 SGTC_207 4185-0010 7.49 4.5 µM 4.29E-14 endomembrane recycling 31 3.46E-14
5 SGTC_2688 kanamycin b 6.52 96.7 µM 4.10E-11 calcium & mitochondrial duress 128 3.45E-11
6 SGTC_2563 tomatine 5.78 8.5 µM 4.26E-9 10 3.70E-9
7 SGTC_2795 7780010 5.28 54.5 µM 7.26E-8 6 6.42E-8
8 SGTC_187 1488-2104 5.10 32.7 µM 1.92E-7 calcium & mitochondrial duress 21 1.71E-7
9 SGTC_201 4262-0159 4.67 12.8 µM 1.68E-6 ergosterol depletion effects on membrane 32 1.52E-6
10 SGTC_292 k038-0015 4.63 474.0 µM 2.03E-6 2 1.83E-6
11 SGTC_2801 7907800 4.56 81.8 µM 2.79E-6 19 2.52E-6
12 SGTC_1923 st058458 4.47 19.1 µM 4.38E-6 21 3.98E-6
13 SGTC_2702 st077768 4.46 96.8 µM 4.43E-6 11 4.03E-6
14 SGTC_568 1193-0097 4.37 7.5 µM 6.72E-6 ERAD & cell cycle 121 6.12E-6
15 SGTC_689 0833-0039 4.34 98.0 µM 7.66E-6 ergosterol depletion effects on membrane 28 6.99E-6
16 SGTC_320 4092-0846 4.23 5.3 µM 1.30E-5 ergosterol depletion effects on membrane 42 1.19E-5
17 SGTC_8 0354-0001 4.19 70.0 µM 1.55E-5 71 1.42E-5
18 SGTC_2915 7971052 4.14 15.2 µM 1.92E-5 4 1.76E-5
19 SGTC_2086 5274770 4.08 176.6 µM 2.49E-5 22 2.29E-5
20 SGTC_2871 9038510 3.99 13.0 µM 3.63E-5 14 3.35E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.309 2.22E-75 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.258 4.39E-52 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.252 8.50E-50 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.241 1.06E-45 YDL001W RMD1 hom Cytoplasmic protein required for sporulation
0.224 2.68E-39 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.215 2.71E-36 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.206 1.94E-33 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.206 2.08E-33 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.206 2.46E-33 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.184 6.62E-27 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.176 1.06E-24 YNL239W LAP3 hom Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
0.175 1.66E-24 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.172 1.28E-23 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.169 8.39E-23 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.168 1.21E-22 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP