Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YGR135W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.309 | 2.22E-75 | YDL148C | NOP14 | het | Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA |
0.258 | 4.39E-52 | YOL065C | INP54 | hom | Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins |
0.252 | 8.50E-50 | YLR021W | IRC25 | hom | Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions |
0.241 | 1.06E-45 | YDL001W | RMD1 | hom | Cytoplasmic protein required for sporulation |
0.224 | 2.68E-39 | YBL010C_p | YBL010C_p | hom | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles |
0.215 | 2.71E-36 | YHL019C | APM2 | hom | Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport |
0.206 | 1.94E-33 | YLR170C | APS1 | hom | Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex |
0.206 | 2.08E-33 | YOR363C | PIP2 | hom | Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication |
0.206 | 2.46E-33 | YFL034W_p | YFL034W_p | hom | Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk |
0.184 | 6.62E-27 | YDR186C | YDR186C | hom | Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
0.176 | 1.06E-24 | YNL239W | LAP3 | hom | Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH |
0.175 | 1.66E-24 | YJL001W | PRE3 | het | Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides |
0.172 | 1.28E-23 | YMR029C | FAR8 | hom | Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p |
0.169 | 8.39E-23 | YER041W | YEN1 | hom | Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p |
0.168 | 1.21E-22 | YDL126C | CDC48 | het | AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP |