YGR140W / CBF2

Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR140W / CBF2

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:2.88|P-value:7.63E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-4.08|P-value:2.95E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.30|P-value:9.10E-7 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.13|P-value:2.53E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.97|P-value:5.74E-15 ID:SGTC_155|Compound:k072-0202|FD-Score:3.99|P-value:5.42E-6 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.04|P-value:4.13E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:2.87|P-value:7.74E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-3.29|P-value:1.29E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.01|P-value:4.48E-4 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:3.45|P-value:7.17E-5 ID:SGTC_559|Compound:1550-0661|FD-Score:2.93|P-value:6.23E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:2.83|P-value:9.27E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.06|P-value:3.75E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:2.99|P-value:5.02E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-2.91|P-value:6.12E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-4.96|P-value:1.92E-8 ID:SGTC_882|Compound:0843-0131|FD-Score:3.36|P-value:1.06E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:2.82|P-value:9.48E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-2.81|P-value:8.91E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.25|P-value:1.37E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.61|P-value:3.41E-5 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.14|P-value:2.67E-4 ID:SGTC_1722|Compound:st034304|FD-Score:5.97|P-value:2.18E-11 ID:SGTC_1723|Compound:st034307|FD-Score:6.63|P-value:1.22E-13 ID:SGTC_1732|Compound:st034308|FD-Score:4.69|P-value:1.14E-7 ID:SGTC_2014|Compound:4013930|FD-Score:3.57|P-value:4.16E-5 ID:SGTC_2158|Compound:5657123|FD-Score:6.49|P-value:3.78E-13 ID:SGTC_2196|Compound:6573537|FD-Score:2.90|P-value:7.05E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.10|P-value:3.15E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.95|P-value:5.69E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.71|P-value:2.14E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-5.22|P-value:3.52E-9 ID:SGTC_2951|Compound:9072950|FD-Score:-3.10|P-value:2.88E-4 ID:SGTC_2973|Compound:9094665|FD-Score:2.88|P-value:7.68E-4 ID:SGTC_2981|Compound:9013952|FD-Score:-2.78|P-value:9.92E-4 ID:SGTC_3127|Compound:9126946|FD-Score:2.86|P-value:8.28E-4 ID:SGTC_3131|Compound:9127911|FD-Score:-3.41|P-value:7.60E-5 ID:SGTC_3158|Compound:9100007|FD-Score:-2.89|P-value:6.71E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.73|P-value:1.94E-5 ID:SGTC_3293|Compound:9120939|FD-Score:4.01|P-value:4.88E-6 ID:SGTC_3320|Compound:9137005|FD-Score:3.26|P-value:1.63E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-2.89|P-value:6.51E-4 ID:SGTC_3342|Compound:9147791|FD-Score:3.01|P-value:4.55E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:2.88|P-value:7.63E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-4.08|P-value:2.95E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.30|P-value:9.10E-7 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.13|P-value:2.53E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.97|P-value:5.74E-15 ID:SGTC_155|Compound:k072-0202|FD-Score:3.99|P-value:5.42E-6 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.04|P-value:4.13E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:2.87|P-value:7.74E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-3.29|P-value:1.29E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.01|P-value:4.48E-4 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:3.45|P-value:7.17E-5 ID:SGTC_559|Compound:1550-0661|FD-Score:2.93|P-value:6.23E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:2.83|P-value:9.27E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.06|P-value:3.75E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:2.99|P-value:5.02E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-2.91|P-value:6.12E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-4.96|P-value:1.92E-8 ID:SGTC_882|Compound:0843-0131|FD-Score:3.36|P-value:1.06E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:2.82|P-value:9.48E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-2.81|P-value:8.91E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:4.25|P-value:1.37E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.61|P-value:3.41E-5 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.14|P-value:2.67E-4 ID:SGTC_1722|Compound:st034304|FD-Score:5.97|P-value:2.18E-11 ID:SGTC_1723|Compound:st034307|FD-Score:6.63|P-value:1.22E-13 ID:SGTC_1732|Compound:st034308|FD-Score:4.69|P-value:1.14E-7 ID:SGTC_2014|Compound:4013930|FD-Score:3.57|P-value:4.16E-5 ID:SGTC_2158|Compound:5657123|FD-Score:6.49|P-value:3.78E-13 ID:SGTC_2196|Compound:6573537|FD-Score:2.90|P-value:7.05E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.10|P-value:3.15E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:2.95|P-value:5.69E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.71|P-value:2.14E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-5.22|P-value:3.52E-9 ID:SGTC_2951|Compound:9072950|FD-Score:-3.10|P-value:2.88E-4 ID:SGTC_2973|Compound:9094665|FD-Score:2.88|P-value:7.68E-4 ID:SGTC_2981|Compound:9013952|FD-Score:-2.78|P-value:9.92E-4 ID:SGTC_3127|Compound:9126946|FD-Score:2.86|P-value:8.28E-4 ID:SGTC_3131|Compound:9127911|FD-Score:-3.41|P-value:7.60E-5 ID:SGTC_3158|Compound:9100007|FD-Score:-2.89|P-value:6.71E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.73|P-value:1.94E-5 ID:SGTC_3293|Compound:9120939|FD-Score:4.01|P-value:4.88E-6 ID:SGTC_3320|Compound:9137005|FD-Score:3.26|P-value:1.63E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-2.89|P-value:6.51E-4 ID:SGTC_3342|Compound:9147791|FD-Score:3.01|P-value:4.55E-4

Top fitness defect scores for YGR140W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1723 st034307 6.63 2.5 µM 1.22E-13 mitochondrial processes 3 1.67E-11 0.34
2 SGTC_2158 5657123 6.49 3.6 µM 3.78E-13 2 4.24E-11 0.26
3 SGTC_1722 st034304 5.97 3.6 µM 2.18E-11 mitochondrial processes 2 1.19E-9 0.02
4 SGTC_1732 st034308 4.69 51.8 µM 1.14E-7 4 1.36E-6 0.30
5 SGTC_1204 4049-0315 4.25 18.3 µM 1.37E-6 8 1.06E-5 0.16
6 SGTC_3293 9120939 4.01 62.0 µM 4.88E-6 10 3.04E-5 0.40
7 SGTC_155 k072-0202 3.99 43.6 µM 5.42E-6 4 3.31E-5 0.20
8 SGTC_3292 9120825 3.73 59.8 µM 1.94E-5 3 9.51E-5 0.08
9 SGTC_2743 auranofin 3.71 4.3 µM 2.14E-5 RPP1 & pyrimidine depletion 13 1.03E-4 0.14
10 SGTC_1258 0671-0043 3.61 57.1 µM 3.41E-5 7 1.52E-4 0.22
11 SGTC_2014 4013930 3.57 200.0 µM 4.16E-5 6 1.79E-4 0.09
12 SGTC_478 N-arachidonylglycine 3.45 10.0 µM 7.17E-5 1 2.81E-4 0.61
13 SGTC_882 0843-0131 3.36 351.0 µM 1.06E-4 18 3.90E-4 0.08
14 SGTC_3320 9137005 3.26 35.1 µM 1.63E-4 endomembrane recycling 8 5.55E-4 0.08
15 SGTC_1445 4239-0156 3.14 97.7 µM 2.67E-4 8 8.36E-4 0.08
16 SGTC_2254 7938975 3.10 95.2 µM 3.15E-4 19 9.60E-4 0.12
17 SGTC_659 k018-0002 3.06 107.0 µM 3.75E-4 17 0.00111 0.04
18 SGTC_418 lividomycin a 3.04 100.0 µM 4.13E-4 9 0.00120 0.01
19 SGTC_473 2-arachidonoylglycerol 3.01 10.0 µM 4.48E-4 17 0.00129 0.01
20 SGTC_3342 9147791 3.01 14.5 µM 4.55E-4 9 0.00130 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.187 8.10E-28 YJR072C NPA3 het Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.174 3.47E-24 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.172 7.80E-24 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.161 6.25E-21 YML098W TAF13 het TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.146 2.25E-17 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.126 2.69E-13 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.124 5.93E-13 YAL034W-A MTW1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
0.123 1.05E-12 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.121 2.39E-12 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.117 1.09E-11 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.117 1.33E-11 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.114 3.65E-11 YGL144C ROG1 hom Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication
0.114 4.03E-11 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.108 4.20E-10 YGR280C PXR1 het Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
0.106 7.83E-10 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)